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Molecular discrimination and transduction in singe olfactory and vomeronasal receptor cells

Research Project

Project/Area Number 09480169
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

KURIHARA Kenzo  Grad. School of Pharm. Sci., Hokkaido University, 大学院・薬学研究科, 教授 (00016114)

Co-Investigator(Kenkyū-buntansha) SHOJI Takayuki  Grad. School of Pharm. Sci., Hokkaido University, 大学院・薬学研究科, 助手 (00241349)
KASHIWAYANAGI Makoto  Grad. School of Pharm. Sci., Hokkaido University, 大学院・薬学研究科, 助教授 (20169436)
MATSUOKA Ichiro  Grad. School of Pharm. Sci., Hokkaido University, 大学院・薬学研究科, 助教授 (40157269)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 1998: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1997: ¥8,600,000 (Direct Cost: ¥8,600,000)
Keywordsolfactory cell / pheromone / turtle / frog / rat / vomeronasal call / IP3 / cAMP / サイクリックAMP / フェロモン受容細胞 / 尿 / イノシトールトリスリン酸 / 二次メッセンジャー / 性成熟 / 嗅線毛 / 鋤鼻器 / イノシトールトリスソン酸
Research Abstract

In order to clarify the odor specificity of a single olfactory cell, various odorants were applied to single olfactory cells of turtle and frog. The results indicated that a single olfactory cell responds to various odorants. In order to examine roles of cyclic GMP in olfactory transduction, cyclic GMP was injected to turtle olfactory cells. The injection induced inward currents. We concluded that the inward currents were induced via cyclic AMP-dependent channel, indicating that pathway specific to cyclic GMP does not exit in turtle olfactory cells. In order to clarify the transduction mechanism of vomeronasal receptor cells, we applied urine, which contains pheromone(s), to rat female vomeronasal cells. Single vomeronasal cell responded only to one species of urine, indicating that a single vomeronasal cell has high specificity. We identified that active components in urine are peptides with molecular weight below 5000. We also tried to identify second messenger in vomeronasal cells. Injection of cyclic AMP to vomeronasal cells induced no response, but injection of inositol trisphosphate (IP3) induced inward currents. Application of inhibitors for generation of IP3 or a blocker of IP3-gated channel blocked the inward currents, suggesting that IP3 is a second messenger in vomeronasal cells.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Inamura, K.: "Inositol 1,4,5-trisphosphate induces responses in receptor neurons in rat vomeronasal sensory slices"Chem, Senses. 22. 93-104 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kashiwayanagi, M.: "Odor discrimination of "cAMP-" and "IP_3-dependent" odorants t high temperature and at high ionoconcentration"J. Physiol, Paris. 91. 1-6 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kashiwayanagi, M.: "Long periodrecording of olfactory and vomeronasal stimulant-induced waves from the turtle main olfactory bulb and accessory olfactory bulb"Brain Ras. Protocols. 1. 287-291 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katuragi, Y.: "Specific inhibitor for bitter taste inhibition of frog taste nerve respones and human taste sensation to bitter stimuli"Brain Res. Protocols. 1. 292-298 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Knaki, K.: "Responses to carnosine, a candidate of transmitter from olfactory neuron, of organotypic slice cultured rat olfatory bulb neurons"Neurosci. Lett.. 231. 167-170 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kashiwayanagi, M.: "Concentration and membrane fluidity dependence of odor discrimination the turtle olfactory system"Chem. Senses. 22. 553-563 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Inamura, K.: "Blockage of urinary responses by inhibitors for IP_3 -mediated pathway in vomeronasal cells"Neurosci. Lett.. 233. 129-132 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Inamura, K.: "Effects of cGMP and sodium nitroprusside on odor responses in turtle olfactory sensory neurons"Am. J. Physiol.. 275. C1201-C1206 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueda, H.: "Lacustrine sockeye salmon return straight to their Natal area from open water using both visual and olfactory cues"Chem. Senses. 23. 207-212 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuoka, I.: "Identification of c-fos related genes and their induction by neral activation in rainbow trout brain"Biochim. Biophys. Acta.. 1395. 220-227 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sasaki, K.: "Inositol-1,4,5-trisphosphate eccumulation induced by urine in rat vomeronaal epithelium"Brain Res.. 823. 161-168 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Inamura, K.: "Laminar distribution of pheromone receptive neurons in rat vomeronasal epihelium"J. Physiol. Lond.. 517. 731-739 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Inamura, K.: "Regionalization of Fos immunostaining in rat accessory olfactory bulb when the vomeronasal organ was exposed to urine"Eur. J. Neurosci.. 11, (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K. Inamura, M. Kashiwayanagi and K. Kurihara: "Inositol 1,4,5-trisphosphate induces responses in receptor neurons in rat vomeronasal sensory slices"Chem. Senses. 22. 93-104 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M. Kashiwayanagi, F. Nagasawa, K. Inamura and K. Kurihara: "Odor discrimination of "cAMP-" and "IPィイD23ィエD2-dependent" odorants at high temperature and at high ion concentration"J. Physil. Paris.. 91. 1-6 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M. Kashiwayanagi, M. Taniguchi, T. Shoji and K. Kurihara: "Long period recording of olfactory and vomeronasal stimulant-induced waves from the turtle main olfactory bulb and accessory olfactory bulb"Brain Res. Protocols.. 1. 287-291 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N. Katsuragi, M. Kashiwayanagi and K. Kurihara: "Specific inhibitor for bitter taste : inhibition of frog taste nerve responses and human taste sensation to bitter stimuli"Brain Res. Protocols.. 1. 292-298 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K. Kanaki, S. Kawashima, M. Hashiwayanagi and K. Kurihara: "Responses to carnosine, a candidate of transmitter from olfactory neuron, of organotypic slice cultured rat olfactory bulb neuron"Neurosci. Lett.. 231. 167-170 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M. Kashiwayanagi, K. Sasaki, A. Iida, H. Saito and K. Kurihara: "Concentration and membrane fluidity dependence of odor discrimination in the turtle olfactory system"Chem. Senses.. 22. 553-563 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K. Inamura, M. Kashiwayanagi and K. Kurihara: "Blockage of urinary responses by inhibitors for IP3-mediated pathway in vomeronasal cells"Neurosci. Lett.. 233. 129-132 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K. Inamura, M. Kashiwayanagi and K. Kurihara: "Effects of cGMP and sodium nitroprusside on odor responses in turtle olfactory sensory neurons"Am. J. Physiol.. 275. C1201-C1206 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H. Ueda, M. Kaeriyama, K. Mukasa, A Urano, H. Kudo, T. Shoji, Y. Tokumitsu, K. Yamauchi and K. Kurihara: "Lacustrine sockeye salmon return straight to their Natal area from open water using both visual and olfactory cues"Chem. Senses.. 23. 207-212 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuoka, K. Fuyuki, T. Shoji, K. Kurihara: "Identification of c-fos related genes and their induction by neural activation in rainbow trout brain"Biochim. Biophys. Acta.. 1395. 220-227 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K. Sasaki, K. Okamoto, K. Inamura, Y. Tokumitsu and M. Kashiwayanagi: "Inositol-1,4,5-trisphosphate accumulation induced by urine in rat vomeronasal epithelium"Brain Res.. 823. 161-168 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K. Inamura, M. Kashiwayanagi and K. Kurihara: "Laminar distribution of pheromone receptive neurons in rat vomeronasal epithelium"J. Physiol. Lond.. 517. 731-739 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K. Inamura, M. Kashiwayanagi and K. Kurihara: "Regionalization of Fos immunostaining in rat accessory olfactory bulb when the vomeronasal organ was exposed to urine"Eur. J. Neurosci.. 11(in Press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Inamura: "Effects of cGMP and sodium vitroprusside on odor responses in turtle olfactory sensory neurons" American Journal of Physiology. 275. c1201-c1206 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Inamura: "Laninar distribution of pheromone receptive neurons in rat vomeronasal epithelium" Journal of Physiology. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Inamura: "Regionalization of Fos immunostaining in rat accesory olfactory bull when the vomeronasal organ was exposed to urted" European Journal of Neuroscience. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Inamura: "Inositol 1,4,5-trisphosphate induces responses in receptor neurons in rat vomeronasal sensory slices." Chem.Senses. 22. 93-104 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Kashiwayanagi: "Odor discrimination of"cAMP"and"IP3-dependent"odorants at high temperature and at high ion concentration." J.Physiol.Paris. 91. 1-6 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Kashiwayanagi: "Long period reco′rding of olfactory and vomeronasal stimulant-induced waves from the turtle main olfactory bulb and accessory olfactory bulb." Brain.Res.Protocols. 1. 287-291 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Kanaki: "Responses to carnosine,a candidate of transmitter from olfactory neuron,of organotypic slice cultured rat olfactory bulb neurons." Neurosci.Lett.231. 167-170 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Kashiwayanagi: "Concentration and membrane fluidity dependence of odor discrimination in the turtle olfactory system." Chem.Sneses.22. 553-563 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Inamura: "Blockage of urinary responses by inhibitors for IP_3-mediated pathway in rat vomeronasal sensory neurons" Neurosci.Lett.233. 129-132 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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