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Ultra-fast X-ray diffraction studies on the relationship between the structun change extensibility of the actin filaments and force generation in muscle

Research Project

Project/Area Number 09480175
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

WAKABAYASHI Katsuzo  Graduate School of Engineering Science, Osaka University, Associate Professor, 基礎工学研究科, 助教授 (00029521)

Co-Investigator(Kenkyū-buntansha) 若林 克三  大阪大学, 基礎工学研究科, 助教授 (00029521)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥10,100,000 (Direct Cost: ¥10,100,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥7,000,000 (Direct Cost: ¥7,000,000)
KeywordsUltra-fast X-ray diffraction / Synchrotron radiation / Contraction of skeletal muscle / Extensibility of actin filaments / Extensibility of muscle filaments / Actin filaments / Myosin filaments / Force generation in muscle / イメージングプレート
Research Abstract

In order to understand the relationship between the mechanical properties of thin actin-containing filaments and force generation in skeletal muscle, the fast X-ray diffraction studies were performed by using synchrotron radiation. The principal results were described below.
(1) The relation between the extensibility of the actin filaments and force generated in muscle was linear, establishing that the actin filament has a purely elastic property. Such an extensibility of the actin filaments was closely associated with a torsional motion of the actin monomers in the filament and its time course of the change occurred with the development of force.
(2) Upon the initial activation, the thin actin filaments became shortened slightly but significantly. Considering this result, the net extensibility of the actin filaments in muscle under the maximum force generation was determined to about 0.35%.
(3) When the ramp length stretch was applied to a contracting muscle, the intensity decrease of the myosin meridional reflections and their axial spacing change run parallel to the change in force, implying that the structural alteration of the myosin crossbridges is coupling with the force-generation mechanism.
In this research, firm experimental evidence was provided for the elastic properties and structural changes of the thin actin filaments in active muscle. Based on these new results, the molecular model for muscle contraction was discussed in a combination of peculiar conformational changes of both actin and myosin.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] 若林克三: "アクチンフィラメントの伸びと筋収縮"日本物理学会誌. 52. 599-605 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa: "Close relationship between the extensibility and twisting of the actin filaments in striated muscles, as studied by X-ray Diffraction"J. Muscle Res. Cell Motil.. 18. 485 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Wakabayashi: "Conformational changes of actin and myosin during force production in muscle studied by X-ray diffraction"Proc. The 22nd Int. Symp. Div. Biophys. Taniguchi Foundation. 283-294 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 若林克三: "トリスタン主リングアンジュレーター光を利用した骨格筋の高分解能X線回折"放射光(日本放射光学会誌). 10. 200-212 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 武澤康範: "放射光X線回折・散乱による筋肉の構造研究"RADIOISOTPES. 47. 776-788 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa: "Extensibility of the actin and myosin filaments in various states of skeletal muscles as studied by X-ray Diffraction"Adv. Exp. Med. & Biol.. 453. 309-317 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa: "Similar effects of the applied stretch and MgADP binding on the structure of skeletal muscle fibers in the rigor state as studied by X-ray diffraction"J. Muscle Res. Cell Motil.. 20. 346 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa: "Backward movements of cross-bridges by application of stretch and by binding of MgADP to skeletal muscle fibers in the rigor state as studied by X-ray diffraction"Biophys. J.. 76. 1770-1783 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 杉本泰伸: "ミオシン・モーター蛋白質のレバーアーム運動とパワーストローク"蛋白質 核酸 酵素. 44. 1605-1612 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Wakabayashi: "Muscle contraction : challenges for synchrotron radiation"J. Synchrotron Rad.. 6. 875-890 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa: "Intensity changes of the first layer-line complex in the X-lay pattern of a frog skeletal muscle during isometric contraction : the intensity of actin-based layer decreases"J. Muscle Res. Cell Motil.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 杉本泰伸: "ニューバイオフィジックス4「生体分子モーターの仕組み」"共立出版. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 上野 豊: "ニューバイオフィジックス7「バイオイメージング」"共立出版. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Wakabayashi et al.: "Extensibility of the actin filaments and muscle contraction"Buturi (J. Phys. Soc. Japan). 52. 599-605 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa et al.: "Close relationship between the extensibility and twisting of the actin filaments in striated muscles, as striated muscles, as studied by X-ray diffraction"J. Muscle Res. Cell Motil.. 18. 485 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Wakabayashi et al.: "Conformational changes of actin and myosin during force production in muscle studied by X-ray diffraction"Proc. the 22nd Int. Symp. Div. Biophys. Taniguchi Foundation. 283-294 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Wakabayashi et al.: "High resolution X-ray diffraction of muscle using undulator radiation from thE Tristan main ring at KEK"Hoshako (Japan. Soc. Synchrotron Rad. Res.). 10. 200-212 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa et al.: "X-ray diffraction/scattering studies of muscle contraction using synchrotron radiation"RADIOISOTPES. 47. 776-788 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa et al.: "Extensibility of the actin and myosin filaments in various states of skeletal muscle as studied by X-ray diffraction"Adv. Exp. Med. & Biol.. 453. 309-317 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa et al.: "Similar effects of the applied stretch and MgADP binding on the structure of skeletal muscle fibres in the rigor state as studied by X-ray diffraction"J. Muscle Res. Cell Motil. 20. 346 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa et al.: "Backward movements of cross-bridges by application of stretch and by binding of MgADP to skeletal muscle fibers in the rigor states as studied by X-ray diffraction"Biophys. J.. 76. 1770-1783 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Sugimoto et al.: "Lever-arm motion and power stroke in muscle motor protein"Protein Nucleic Acid Enzyme. 44. 1605-1612 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Wakabayashi et al.: "Muscle contraction : challenges for synchrotron radiation"J. Synchrotron Rad.. 6. 875-890 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa et al.: "Intensity changes of the first layer-line complex in the X-ray pattern of a frog skeletal muscle during isometric contraction : the intensity of actin-based layer line decreases"J. Muscle Res. Cell Motil. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Takezawa: "Similar effects of the applied stretch an MgADP binding on the structure of skeletal muscle fibres in the rigor state as studied by X-ray diffraction"J. Muscle Res. Cell Motil.. 20. 336-337 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Arata: "X-ray solution scattering study of the actin dimer-myosin head complex"J. Muscle Res. Cell Motil.. 20. 335-336 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 杉本泰伸: "ミオシン・モーター蛋白質のレバーアーム運動とパワーストローク"蛋白質 核酸 酵素. 44. 1605-1612 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Wakabayashi: "Muscle contraction : challenges for synchrotron radiation"J. Synchrotron Rad.. 6. 875-890 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Takezawa: "Intensity changes of the first layer-line complex in the X-ray pattern of a frog skeletal muscle during isometric contraction : the intensity of actin-based layer line decreases"J. Muscle Res. Cell Motil.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Wakabayashi: "Muscle Contraction Mechanism : use of X-ray diffraction"Encyclopedia of Life Sciences. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Wakabayashi: "High-Resolution X-Ray Diffraction of Muscle Using Undulator Radiation from the Tristan Main Ring at KEK" J.Synchrotron Rad.5. 280-285 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] D-S.Kim: "X-Ray Diffraction Studies on the Structural Changes of Rigor Muscles Induced by Binding of Phosphate Analogs in the Presence of MgADP" Biophys.Chem.74. 71-78 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 武澤康範: "放射光X線回折・散乱による筋肉の構造研究" RADIOISOTOPES. 47. 776-788 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Takezawa: " Extensibility of the Actin and Myosin Filaments in Various States of Skeletal Muscle as Studiesd by X-Ray Diffraction" Adv.Exp.Med.Biol.453. 309-317 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Arata: "Structure of the Monomeric Actin-Myosin Head Complex as Revealed by X-Ray Solution Scattering" Adv.Exp.Med.Biol.453. 73-78 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Wakabayashi: "Structural Dynamics of Actomyosin in Muscle Contraction by X-Ray Diffraction KEK Ann.Rep.1998 In press" KEK Ann.Rep.(In press). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Tajima: "The Overlap between the Thin and Thick Filament Reflections in the Small-Angle X-Ray Diffraction Pattern from a Molluscan Smooth Muscle" J.Synchrotron Rad.5(In press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Takezawa: "Backward Movements of Cross-Bridges by Application of Stretch and by Binding of MgADP to Skeletal Muscle Fibers in the Rigor States as Studied by X-Ray Diffraction" Biophys.J.76(In press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 若林克三: "アクチンフィラメントの伸びと筋収縮" 日本物理学会誌. 52. 599-605 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 若林克三: "トリスタン主リングアンジュレーター光を利用した骨格筋の高分解能X線回折" 放射光(日本放射光学会誌). 10. 108-120 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 杉本泰伸: "X線でとらえた分子モーターの形態変化" 「生体分子モーターの仕組み」シリーズニューバイオフィジックス 共立出版. 第4巻. 86-103 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Wakabayashi: "Conformational changes of actin and myosin during force production in muscle studied by X-ray diffraction" Structure and Function of Macromolecular Assembly edited by K.Namba,Taniguchi Foundation. 283-294 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 上野 豊: "シンクロトロン放射光による動的X線回折" 「バイオイメージング」シリーズニューバイオフィジックス 共立出版. 第7巻. 170-194 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Wakabayashi: "High-Resolution X-ray Diffraction of Muscle Using Undulator Radiation from the Tristan Main Ring at KEK" J.Synchrotron Rad.5(In press). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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