| Project/Area Number |
09480197
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
HOSOYA Hiroshi HIROSHIMA UNIV,BIOLOGICAL SCIENCE,PROFESSOR, 理学部, 教授 (90183102)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥3,900,000 (Direct Cost: ¥3,900,000)
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| Keywords | Myosin II / MLCK / phosphorylation / Myosin / HeLa Cell / Cell Division / Cytokinesis / 細胞管分裂 / ミオシン / MeLa細胞 / 細胞質分裂 |
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| Research Abstract |
A novel myosin light chain kinase cDNA was isolated from HeLa cells cDNA library. The deduced amino acid sequence was identical to that of zipper interacting protein kinase (ZIPK) which mediated apoptosis (Kawai et. al. Mcl. Cell. Bxol. 18, 1642-1651, 1998). Here we found that HeLa ZIPK phosphorylated myosin II regulatory light chain. (MRLC) at both serine 19 and threonine 18 in ca^2^+/calmodulin independent manner. Phosphorylation of myosin II by HeLa ZIPK resulted in activation of actin-activated MgATPase activity of myosin II.HeLa ZIPK is the first kinase from nonmuscle cells that phosphorylates MRLC at two sites.
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