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Membrane phospholipid dynamics and cytoskeletal organization: Regulation of actin filament assembly by redistribution of membrane surface phospholipid

Research Project

Project/Area Number 09480201
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionTokyo Metropolitan Organization for Medical Research

Principal Investigator

UMEDA Masato  The Tokyo Metropolitan Institute of Medical Science, Department of Molecular Biodynamics, 東京都臨床医学総合研究所, 研究員 (10185069)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Ken-ichi  The Tokyo Metropolitan Institute of Medical Science, Department of Molecular Biodynamics, 東京都臨床医学総合研究所, 研究員 (70270684)
ITOH Kouichi  The Tokyo Metropolitan Institute of Medical Science, Department of Molecular Biodynamics, 東京都臨床医学総合研究所, 研究員 (30291149)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥5,400,000 (Direct Cost: ¥5,400,000)
Keywordsphospholipid / cytoskeleton / lipid bilayer / membrane / neuron / actin / yeast / phosphatidylethanolamine / ニューロン / マクチン / 変異体 / 変異株
Research Abstract

Using a novel peptide probe, Ro09-0198, which specifically forms a tight equimolar complex with phosphatidylethanoalmaine (PE) on biological membranes, we have analyzed the cell surface movement of PE in dividing CHO cells. We found that flip-flop of plasma membrane PE was markedly enhanced at the cleavage furrow during the late telophase of cytokinesis and that cell surface trapping of PE induced by adding the peptide-streptavidin complex to the mitotic cells blocked cytokinesis at the late telophase. Further analysis have shown that the redistribution of plasma membrane phospholipids acts as a regulator of actin filament assembly and play a crucial role in mediating a coordinate movement between plasma membrane and cytoskeleton. Molecules involved in the cross-talk between the phospholipid dynamics and cytoskeletal reorganization have been investigated using yeast and CHO mutant cells which were selected by their altered sensitivity to the PE-binding peptide.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Emoto, K., et al.: "Exposure of phosphatidylethanolamine on the surface of apoptotic cells"Exp.Cell Res.. 232. 430-434 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Kaneda, et al. Umeda,M. et al.: "Localization of the phosphatidylserine-binding site of glyceraldehyde-3-phosphate dehydrogenase responsible for membrane fusion"J.Biochem.. 122. 1233-1240 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A.Yamaji, et al: "Lysenin,a novel sphingomyelin-specific binding protein"J.Biol.Chem.. 273. 5300-5306 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Uchida, et al.: "Induction of apoptosis by phosphatidylserine"J.Biochem.. 123. 1073-1078 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Hanada, et al.: "Mammalian cell mutants resistant to a sphingomyelin-directed bytolysin"J.Biol.Chem.. 273. 33787-33794 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Bogdanov, et al.: "Phospholipid-assisted refolding of an integral membrane protein : Minimun structural features for phosphatidylethanolamine to act as a molecular chaperone"J.Biol.Chem.. 274. 12339-12345 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Emoto,K., et al.: "Isolation of a Chinese hamster ovary cell mutant defective in intramitochondrial transpors of phosphatidylserine"Proc.Natl.Acad.Sci.USA. 96. 12400-12405 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Umeda,M. et al.: "Membrane phospholipid dynamics during cytokinesis : Regulation of actin filament assembly by redistribution of membrane surface phospholipids"Chemistry and Physics of Lipids,Review. 101. 81-91 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoh, O., et al.: "Membrane structure of hepatitis B virus surface antigen particle"J. Biochem. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kobayashi,H.et al.: "Lethal and non-lethal responses of spermatozoa from a wide variety of vertebrates and invertabrates to lysenin, a protein from the coelomic fluid of the earthworm Eisenia foetida"J.Exp. Zoology, in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 榎本和生、小林俊秀、梅田真郷: "形質膜スフィンゴ脂質の分布、動態と細胞機能"実験医学. 15. 1470-1475 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 榎本和生、梅田真郷: "生体膜脂質の非対称分布とその生理的意義"蛋白質核酸酵素. 43. 1973-1980 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Emoto, N.Toyama-sorimachi, H.Karasuyama, K.Inoue, M.Umeda: "Exposure of phosphatidylethanolamine on the surface of apoptotic cells"Exp. Cell Res.. 232. 430-434 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Kobayashi, Y.Sekizawa, S. Shinoda, S.Natori, T.Nakajima, M.Umeda: "Lysenin, a novel bioactive protein isolated from coelomic fluid of the earthworm Eisenia foetida: Structure, secretion and biological activity. Neurobiology: Prospect and Perspectives"Springer-Verlag, Heidelberg. 255-269 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Kaneda, K.Takeuchi, K.Inoue, M.Umeda: "Localization of the phosphatidylserine-binding site of glyceraldehyde-3-phosphate dehydrogenase responsible for membrane fusion"J. Biochem.. 122. 1233-1240 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A.Shiratsuchi, M.Umeda, Y.Ohba, Y.Nakanishi: "Recognition of phosphatidylserine on the surface of apoptic spermatogenic cells and subsequent phagocytosis by Sertoli cells of the rat."J. Biol. Chem.. 272. 2354-2358 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A.Yamaji, Y.Sekizawa,K.Emoto, H.Sakuraba, K.Inoue, H.Kobayashi, M.Umeda: "Lysenin, a novel sphingomyelin-specific binding protein"J. Biol. Chem.. 273. 5300-5306 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Uchida, K.Emoto, D.L.Daleke, K.Inoue, M.Umeda: "Induction of apoptosis by phosphatidylserine."J. Biochem.. 123. 1073-1078 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] I.V.Boronenkov, J.C.Loijens, M.Umeda, R.A.Anderson: "Phosphoinositide signaling pathways in nuclei are associated with nuclear speckles containing pre-mRNA processing factors."Mol. Biol. Cell. 9. 3547-3560 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Hanada, T.Hara, M.Fukasawa, A.Yamaji, M.Umeda, M.Nishijima: "Mammalian cell mutants resistant to a sphingomyelin-directed bytolysin."J. Biol. Chem.. 273. 33787-33794 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A.Ikemoto, T.Kobayashi, K.Emoto, M.Umeda, S.Watanabe, H.Okyuyama: "Effects of docosahexaenoic and arachidonic acids on the synthesis and distribution of aminophospholipids during neuronal differentiation of PC12 cell."Arch. Biochem. Biophys.. 364. 67-74 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Umeda, K.Emoto: "Membrane phospholipid dynamics during cytokinesis: Regulation of actin filament assembly by redistribution of membrane surface phospholipids."Chemistry and Physics of Lipids, Review. 101. 81-91 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Emoto, O.Kuge, M.Nishijima, M.Umeda: "Isolation of a Chinese hamster ovary cell mutant defective in phosphatidylserine translocation into mitochondria."Proc.Natl. Acad.Sci. USA. 96. 12400-12405 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Emoto, K., et al.: "Isolation of a Chinese hamster ovary cell mutant defective in intramitochondrial transport of phosphatidylserine"Proc. Natl. Acad. Sci. USA. 96. 12400-12405 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Umeda, M. et al.: "Membrane phospholipid dynamics during cytokinesis : Regulation of actin filament assembly by redistribution of membrane surface phospholipids"Chemistry and Physics of Lipids, Review. 101. 81-91 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Satoh, O. et al.: "Membrane structure of hepatitis B virus surface antigen particle"J. Biochem.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Kobayashi, H. et al.: "Lethal and non-lethal responses of spermatozoa from a wide variety of vertebrates and invertabrates to lysenin, a protein from the coelomic fluid of the earthworm Eisenia foetida"J. Exp. Zoology. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 梅田真郷、榎本和生: "膜融合におけるリン脂質ダイナミクスと細胞骨格制御"生物物理. 21. 18-23 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 榎本和生、加藤詩子、梅田真郷: "リン脂質の細胞膜動態と細胞分裂制御膜と細胞骨格系の新たなクロストーク"蛋白質核酸酵素. 44. 1173-1180 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 山路顕子ら: "Lysonin,anovel sphingomyelin-specific bury protcin" J.Biol.Chem.273. 5300-5306 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 内田佳予子ら: "Iuduction of apoptosis by phosphatidylserine" J.Biochem. 123. 1073-1078 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 榎本和生ら: "細胞膜脂質の非対称分布とその生理的意義" 蛋白質核酸酵素. 43. 1973-1980 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 榎本和生ら: "細胞膜の構造と機能" 丸善出版, 18 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 榎本和生 ら: "Exposure of phosphatidylethanolamine on the surfau of apoptotic cells" Exp.Cell.Res.232. 430-434 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 兼田瑞穂 ら: "Localization of the phosphatidylserine-binding site of ggoreldaydo-3-phosphrte dehy drogerase responsiele for membrane forsion" J.Biochem.122. 1233-1240 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山路顕子 ら: "Lysenin,a novel sphingomyelin-specific binding protein." J.Biol.Chem.273. 5300-5306 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 内田桂予子 ら: "Induction of apoptosis by phosphotidylserine" J.Biol.Chem.(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 榎本和生 ら: "形質膜スフィンゴ脂質の分布.動態と細胞機能" 実験医学. 15. 1470-1475 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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