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Network formation of motoneurons

Research Project

Project/Area Number 09480207
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionKumamoto University

Principal Investigator

TANAKA Hideaki  Division of Developmental Neurobiology, Kumamoto University Graduate School of Medical Sciences, Professor, 医学研究科, 教授 (90106906)

Co-Investigator(Kenkyū-buntansha) TONO-OKA Shiro  Identical with the H.I., Research Associate, 医学研究科, 助手 (10284754)
KIMURA Yoshishige  Identical with the H.I., Research Associate, 医学研究科, 助手 (90274703)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 1998: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1997: ¥8,400,000 (Direct Cost: ¥8,400,000)
KeywordsMotoneuron / network formation / Eph / receptor tyrosine kinase / ephrin / axonal guidance / Eph受容体型チロシンキナーゼ / 阻害モノクローナル抗体 / 軸索成長 / ガイダンス
Research Abstract

We previously found that two of the Eph receptor tyrosine kinases, EphA3 and EphA4, are transiently expressed in subsets of chick embryonic motoneurons and that the Eph ligands, ephrin-A2 and ephrin-A5, inhibit neurite outgrowth from the motoneurons in culture. Here we developed functional blocking monoclonal antibodies against EphA3, EphA4 and ephrin-A2 in order to examine their functional roles in axonal guidance of motoneurons in vivo. We raised many monoclonal antibodies against the chimeric proteins of each extracellular domain and Fc region of human IgG.Among them some antibodies had blocking activity for ligand-receptor binding and functional blocking activities in stripe assay of retinotectal system. The epitopes of anti-EphA4 and anti-ephrin-A2 were determined to be localized at cysteine rich domain and N-terminal region, respectively. These domains appeared to be essential for Eph receptor-ligand binding. We are now examining the effects of these functional blocking antibodies on motoneuron axonal growth in chick embryos.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Tanaka Hideaki: "Commitment of motoneuron progenitors of chick embryo along the anterior-posterior axis." Developmental Neuroscience. 19.1. 106-111 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka Hideaki: "Low-affinity nerve growth factor receptor is associated with motoneuron axonal pathways." Neuroscience Research. 27・1. 21-27 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 藤井智子: "細胞接着蛋白質、SCI" 蛋白質 核酸 酵素. 42・3. 552-554 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka Hideaki: "Inhibitory effect of Eph receptor tyrosine kinase on motoneuron axonal growth." In"Molecular basis of axon growth and nerve pattern formation"(ed.Fujisawa,H.)Taniguchi Symposia on Brain Sciences. 20. 257-265 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Monschau Bruno: "Shared and distinct functions of RAGS and ELF-1 in guiding retinal axons." The EMBO Journal. 16・6. 1258-1267 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohta Kunimasa: "The inhibitory effect on neurite outgrowth of motoneurons exerted by the ligands ELF-1 and RAGS." Mechanisms of Development. 64・1-2. 127-135 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirano Shigeki: "Normal ontogenic observations on the expression of Eph receptor tyrosine kinase,Cek8,in chick embryos." Anatomy & Embryology. 197・3. 187-197 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wada Naoyuki: "Glycosylphosphatidylinositol-anchored cell surface proteins regulate position-specific cell affinity in the limb bud." Developmental Biology. 202・2. 244-252 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Koo Suzzano J.: "Species-specific immunostaining of embryonic corneal nerves : Techniques for inactivating endogenous peroxidases and demonstration of lateral diffusion of antibodies in the plane of the corneal stroma." Journal of Neuroscience Methods. 85・1. 63-71 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka, H.: "Commitment of motoneuron progenitors of chick embryo along the anterior-posterior axis." Dev.Neurosci.19 : 1. 106-111 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka, H.: "Low-affinity nerve growth factor receptor is associated with motoneuron axonal pathways." Neurosci.Res.27 : 1. 21-27 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka, H.: "Inhibitory effect of Eph receptor tyrosine kinase on motoneuron axonal growth. In "Molecular basis of axon growth and nerve pattern formation"" Taniguchi Symposia on Brain Sciences. No.20. 257-265 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Monschau, B.: "Shared and distinct functions of RAGS and ELF-1 in guiding retinal axons." EMBO J.16 : 6. 1258-1267 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohta, K.: "The inhibitory effect on neurite outgrowth of motoneurons exerted by the ligands ELF-1 and RAGS." Mech.Dev.64 : 1-2. 127-135 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirano, S.: "Normal ontogenic observations on the expression of Eph receptor tyrosine kinase, Cek8, in chick embryos." Anat.Embryol.197 : 3. 187-197 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wada, N.: "Glycosylphosphatidylinositol-anchored cell surface proteins regulate position-specific cell affinity in the limb bud." Dev.Biol.202 : 2. 244-252 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Koo, S.J.: "Species-specific immunostaining of embryonic corneal nerves : Techniques for inactivating endogenous peroxidases and demonstration of lateral diffusion of antibodies in the plane of the corneal stroma." J.Neurosci.Methods. 85 : 1. 63-71 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirano Shigeki: "Normal ontogenic observations on the expression of Eph receptor tyrosine kinase,Cek8,in chick embryos." Anatomy & Embryology. 197・3. 187-197 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wada Naoyuki: "Glycosylphosphatidylinositol-anchored cell surface proteins regulate position-specific cell affinity in the limb bud." Developmental Biology. 202・2. 244-252 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Koo S.J.: "species-specific immunostaing of embryonic corneal nerves." Journal of Neuroscience Methods. 85・1. 63-71 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanaka Hideaki: "Commitment of motoneuron progenitors of chick embryo along the anterior-posterior axis." Developmental Neuroscience. 19・1. 106-111 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanaka Hideaki: "Low-affinity nerve growth factor receptor is associated with motoneuron axonal pathways." Neuroscience Research. 27・1. 21-27 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Monschau Bruno: "Shared and distinct functions of RAGS and ELF-1 in guiding retinal axons." The EMBO Journal. 16・6. 1258-1267 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohta Kunimasa: "The inhibitory effect on neurite outgrowth of motoneurons exerted by the ligands ELF-1 and RAGS." Mechanisms of Development. 64・1-2. 127-135 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hirano Shigeki: "Normal ontogenic observations on the expression of Eph receptor tyrosine kinase, Cek8, in chick embryos." Anatomy & Embryology. 197・3. 187-197 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanaka Hideaki: "Molecular basis of axon growth and nerve pattern formation" 学会出版センター/S. Karger 刊, 9 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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