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Molecular mechanism for synaptic plasticity mediated by postsynaptic density

Research Project

Project/Area Number 09480222
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionShinshu University

Principal Investigator

SUZUKI Tatsuo  Shinshu University School of Medicine, Professor, 医学部, 教授 (80162965)

Co-Investigator(Kenkyū-buntansha) OKANO Akira  Shinshu University School of Medicine, Research Associate, 医学部, 助手 (30020803)
TAKAGI Hiroshi  Shinshu University School of Medicine, Associate Professor, 医学部, 講師 (80247220)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥10,900,000 (Direct Cost: ¥10,900,000)
KeywordsPSD / postsynaptic / synaptic plasticity / cloning
Research Abstract

1. We performed expression cloning of cDNAs using antibody reacting to postsynaptic density (PSD) proteins. We cloned known clones such as those encoding fodrin, plectin and neurofilament, as well as unknown clones. We further proceeded the cloning of unknown clones and isolated a cDNA encoding a synGAP isoform, which we termed as synGAPβ. Furthermore, we identified 7 synGAPmRNAs (splice variants) and 5 synGAP protein isoforms. The synGAPβ protein was found to be specifically localized in the PSD fraction isolated form the rat brain.
2. We have isolated another novel gene from a rat cDNA library. The cDNA encoded 3960 bases and contained 2903 base ORF. The speculated amino acid sequence had homology to talin and Sla2 in the carboxyl terminal half region, and to Huntingtin-interacting protein (HIP) in the whole region. Northern blotting showed 4.2 kb band and distribution in the brain and the testis. The protein was detected by Western blotting in the spleen as well as the brain and the testis, and synaptosome and the synaptic plasma membrane fractions, among subcellular fractions prepared from the rat brain. The distribution of the protein resembled that of Huntingtin: These results suggested roles for synaptic function and those related to Huntingtin in the brain.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] 鈴木 龍雄: "シナプスのMAPキナーゼ系"生体の科学. 48. 97-100 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki,T.: "Presence of both NF-KB-like and IKB-like immunoreactivities in postsynaptic densities in the rat brain"Neuroreport. 8. 2931-2935 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki,T.: "Localization of α-internexin in the postsynaptic density of the rat brain"Brain Res.. 765. 74-80 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki,T.: "Occurrence of a transcription factor, cAMP response element-binding protein(cREB), in the postsynaptic sites of the brain"Mol.Brain Res.. 61. 69-77 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe,S.: "Differential roles of two types of voltage-gated Ca^<2+> channels in the dendrites of rat cerebral Purkinje Neurons"Brain Res.. 791. 43-45 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 小谷 進: "同時多点計測法により観察される海馬の長期増強現象"10. 124 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takagi,H.: "Roles of A-and D-type k channels in EPSP integration at a model dendrite"Neurosci.Lett. 254. 165-168 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 高木 博: "記憶モデルとしての海馬LTPについて"放射線科学. 42. 205-211 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki,T.: "Presence of molecular chaperones, heat shock cognate(Hsc)70 and heat shock protein(Hsp)40, in the postsynaptic structures of rat brain"Brain Res.. 816. 99-110 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tian,QB: "Identification of mRNAs localizing in the postsynaptic region"Mol.Brain Res.. 72. 147-157 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki,T.: "Presence of up-stream and down-stream components of mitogen-activated protein kinase(MAPK)pathway in the PSD fraction of the rat forebrain"Brain Res.. 840. 36-44 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tojima,T.: "Fine inside structure of exocytotic apertures in nerve cells revealed by atomic force microscopy"Biophys.J.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takagi,H.: "Roles of k channels in EPSP integration at model dendrite"Neurosci.Res.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 鈴木龍雄: "Bio Science,用語ライブラリー・脳・神経"羊土社. 248(2) (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki T et al.: "Localization of α-internexin in the postsynaptic density of the rat brain."Brain Res. 765. 74-80 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki T et al.: "Presence of NF-κB-like and lκB-like immunoreactivities in postsynaptic densities."Neuroreport. 8. 2931-2935 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki T et al.: "Occurrence of a transcription factor, cAMP response element-binding protein (CREB), in the postsynaptic sites of the brain."Mol. Brain Res. 61. 69-77 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe S et al.: "Differential Roles of Two Types of Voltage-Gated CaィイD12+ィエD1 Channels in the Dendrites of Rat Cerebellar Purkinje Neurons."Brain Res.. 791. 43-55 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takagi H et al.: "Roles of A- and D-type K channels in EPSP integration at a model dendrite."Neurosci. Lett.. 254. 65-168 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki T et al.: "Presence of molecular chaperones, heat shock cognate (Hsc) 70 and heat shock protein (Hsp) 40, in the postsynaptic structures of rat brain."Brain Res. 816. 99-110 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tian QB et a l.: "Identification of mRNAs localizing in the postsynaptic region."Mol Brain Res. 72. 147-157 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki T et al.: "Presence of up-stream and down-stream components of mitogen-activated protein kinase (MAPK) pathway in the PSD fraction of the rat forebrain."Brain Res. 840. 36-44 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tojima, T et al.: "Fine inside structure of exocytotic apertures in nerve cells revealed by atomic force microscopy."Biophys. J.. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takagi H: "Role of K channels in EPSP integration at model dendrite."Neurosci Res. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki, T: "Presence of molecular chaperomes, heat shock cognate (Hsc) 70 and heat shock protein (Hsp)40,in the postsynaptic structures of rat brain."Brain Res.. 816. 99-110 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tian, QB: "Identification of mRNAs localizing in the postsynaptic region."Mol. Brain Res.. 72. 147-157 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki, T: "Presence of up-stream and down-stream conponents of mitogen-activated protein Kinase(MAPK) pathway in the PSD fraction of the rat forebrain."Brain Res. 840. 36-44 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tojima, T: "Fine inside structure of exocytotic apertures in nerve cells revealed by atomic force microscopy."Biophys. J.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takasi, H: "Roles of K channels in EPSP integration at model dendrite."Neurosci. Res. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki,T: "Occurrence of a transcription factor, cAMP response element-binding protein (CREB) , in the pistsynaptic sites of the brain" Mol.Brain.Res.61. 69-77 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suzuki, T: "Presence of molecular chaperones, heat shoch cognate (Hsc) 70 and heat shock proteint (Hsp) 40, in the postsynaptic structures of rat brain" Brain Res. in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Watanabe, S: "Differential roles of two types of voltage-gated Ca^<2H+> channels in the dendrites of rat cerelral Purkinje Neurons" Brain Res.791. 43-45 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小谷,進: "同時多点計測法により観察される海馬の長期増強現象" J.of Advanced Science. 10. 124 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takagi,H: "Roles of A- and D- type K channels in EPSP integration at a model dendrite." Neurosci.Lett. 254. 165-168 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 高木,博: "記憶モデルとしてのLTPについて" 放射線科学. in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 鈴木龍雄: "シナプスのMAPキナーゼ系" 生体の科学. 48. 97-100 (1977)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suzuki,T: "Presence of both NF-kB-like and IkB-like immunoreactivities in postsynapric densities in the rat brain." Neuroreport. 8. 2931-2935 (1977)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suzuki,T.: "Localization of α-internexin in the postsynaptic density of the rat brain." Brain Res.765. 74-80 (1977)

    • Related Report
      1997 Annual Research Report
  • [Publications] 鈴木龍雄: "Bio Science用語ライブラリー・脳・神経" 羊土社, 248(2) (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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