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Analysis using optical recording for synaptic plasticity of non-LTP type in hippocampal slices

Research Project

Project/Area Number 09480227
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionGunma University, School of Medidcine

Principal Investigator

SEKINO Yuko  Gunma Univ. Shc. Med. Dept. of Neurobiology and Behavior, Assist. Professor, 医学部, 講師 (70138866)

Co-Investigator(Kenkyū-buntansha) SHIRAO Tomoaki  Gunma Univ. Shc. Med. Dept. of Neurobiology and Behavior, Professor, 医学部, 教授 (20171043)
HAYASHI Kensuke  Gunma Univ. Shc. Med. Dept. of Neurobiology and Behavior, Research Associate (50218567)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsHippocampus / Optical Recording / Adenosine / LTP / CA2 / CA3a / 長期増強 / 光学的測定 / 膜電位感受性色素 / 海馬スライス / 光学的測定法 / アデノシンA1受容体
Research Abstract

Using optical and field potential recordings, we have shown that there were two distinguished patterns of signal propagation, CA3-CA1 and CA3-CA2-CA1, in oblique slices obtained from rat hippocampus ; the, and the slow propagation. In the fast propagation, activity flowed directly from CA3 to the stratum radiatum of CA1, and in the slow propagation optical signals were generated in CA2 before spreading to CA1. Our data reveal that CA2 neurons can transmit delayed excitatory signals to CA1 neurons. We therefore conclude that consideration of electrical signal propagation through the hippocampus should include flow through the CA2 region in addition to the traditional dentate gyrus-CA3-CA1 trisynaptic circuit. When slices were treated with an adenosine antagonist (2 μM 8-CPT), population spikes (PSs) were recorded in the stratum pyramidale of CA1 and CA2 in almost all of transverse slices, where no PS was recorded without 8-CPT in most cases. This data indicated that there is strong puri … More nergic inhibition in CA2/CA3a and suggest that CA2 cells act as a gate of signal propagation in the transverse plane of hippocampus.
We further described the immunocytochemical distribution of adenosine A1 receptors in the rat hippocampus. Adenosine A1 receptor-like immunoreactivity was seen on the cell soma and dendrites of pyramidal cells and the cell soma and proximal part of dendrites of granule cells, but not on glial cells. In the adult rat hippocampus, rostrocaudal inspection revealed that immunoreactivity in CA2/CA3a was greatest. Confocal microscopy revealed differences in the staining patterns for the adenosine A1 receptor and synaptophysin, a marker of presynaptic terminals. This suggests that the adenosine A1 receptor might have postsynaptic physiological functions. Double-labeling of adenosine A1 receptors and anterogradely labeled fibers from the supramammillary nucleus showed that the fibers from the supramammillary nucleus terminate directly on the cell soma of the A1 receptor immunopositive neurons in CA2/CA3a and the dentate gyrus.
These results indicate that the adenosine A1 receptor in CA2/CA3a and the dentate gyrus are in a position to regulate hippocampal theta activity and that resultant strong synaptic depression in CA2/CA3a could play a role in regulating the intrinsic signal flow between CA3 and CA1. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] TOMOYO OCHIISHI,LING CHEN,AYA YUKAWA,YOSHIKO SAITOH,YUKO SEKINO,TAKAO ARAI,HIROYASU NAKATA,HIROSHI MIYAMOTO: "Cellular Localization of Adenoshine Al Receptors in Rat Forebrain : Immunohisto chemical Analysis Using Adenosine AI Receptor-Specific Monoclonal Antibody"THE JOURNAL OF COMPARATIVE NEUROLOGY. 411. 301-316 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasuji Matsuoka,Mitsuhiro Okazaki,Kazuyuki Takata,Yoshihisa Kitamura,Shyunsaku Ohta,Yuko Sekine,Takashi Taniguchi: "Endogenous adenosine protects CA1 neurons from kainic acid-induced neuronal cell loss in the rat hippocampus"European Journal of Neuroscience. Vol.11. 3617-3625 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.OCHIISHI,Y.SAITOH,A.YUKAWA,M.SAJI,Y.REN,T.SHIRAO,H.MIYAMOTO,H.NAKATA,Y.SEKINO: "HIGH LEVEL OF ADENOSINE A1 RECEPTOR-LIKE IMMUNOREACTIVITY IN THE CA2/CA3a REGION OF THE ADULT RAT HIPPOCAMPUS"Neuroscience. 93・3. 955-967 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoshi Fujii.Yuko Sekino,Yoichiro Kuroda,Hiroshi Sasaki,Ken-ichi Ito,Hiroshi Kato: "8-cyclopenthyl theophylline an adenosine A receptor antagonist, inhibits the reversed of long-term potentiation in hippocampal CA1 neurons"European Journal of Pharmacology. 331. 9-14 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] YUKO SEKINO,KUNIHIRO OBATA,MANABU TANIFUJI,MAKOTO MIZUNO,JIN MURAYAMA: "Delayed Signal Propagation via CA2 in Rat Hippocampal Slices Revealed by Optical Recording"J. Neurophysiology. 78. 1662-1668 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Saji,S.Kobayashi,K.Ohno,Y.Sekino: "Interruption of Supramammillo-hippocampal afferonts prevents the genesis and spread of limbic seizures in the hippocampus via a disinhibition mechanism"Neuroscience. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 関野祐子・吉岡亨: "ニューバイオフィジックス 5巻 イオンチャネル"生物物理学会. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Saji, S. Kobatashi, K. Ohno, Y. Sekino: "Interruption of supramammillo-hippocampal afferents prevents the genesis and spread of limbic seizures in the hippocampus via a disinhibition mechanism"Neurosci.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Matsuoka, M. Okazaki, K. Tanaka, Y. Katamura, S. Ohta, Y. Sekino, T. Taniguchi: "Endogenous Adenosine Protects CA1 Neurons from Kainic Acid-Induced Neuronal Cell Loss in the Rat Hippocampus"Eur. J. Neurosci.. 11. 3617-3625 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Ochiishi, Y. Saitoh, A. Yukawa, M. Saji, Y. Ren, T. Shirao, H. Miyamoto, H. Nakata and Y. Sekino: "High level of Adenosine A1 Receptor-like Immunoreactivity in CA2/CA3a of the Adult Rat Hippocampus"Neuroscience. 93. 955-967 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Ochiishi, L. Chen, A. Yukawa, Y. Saitoh, Y. Sekino, T. Arai, H. Nakata, H. Miyamoto: "Cellular Localization of Adenosine A1 Receptors in Rat Forebrain : Immuno histochemical Analysis Using Adenosine A1 Receptor-Specific Monoclonal Anitibody"J. Comp. Neurol.. 411. 301-316 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Sekino, K. Obata, M. Tanifuji, M. Mizuno and J. Murayama: "Delayed Signal Propagation via CA2 in Rat Hippocampal Slices Revealed by Optical Recording. 6."J. Neurophysiol.. 78. 1662-1668 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Fujii, Y. Sekino, Y. Kuroda, H. Sasaki, K. Ito and H. Kato: "8-Cyclopentyltheophylline, an Adenosine A1 Receptor Antagonist, Inhibits the Reversal of Long-term Potentiation in Hippocampal CA1 Neurons."Eur. J. Pharmacol.. 331. 9-14 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] TOMOYO OCHISHI, LING CHEN, AYA YUKAWA, YOSHIKO SAITOH, YUKO SEKINO, TAKAO ARAI, HIROYASU NAKATA, HIROSHI MIYAMOTO: "Cellular Locarization of Adenosine A1 Receptors in Rat Forebrain : Immunohistochemical Analysis Using Adenosine A1 Receptor-Specific Monoclonal Antibody"THE JOURNAL OF COMPARATIVE NEUROLOGY. 411. 301-316 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasuji Mtsuoka, Mitsuhiro Okazaki, Kazuyuki Takata, Yoshihisa Kitamura, Shynsaku Ohta, Yuko Sekino, Takasaki Taniguchi: "Endogenous adenosine protects CAI neurons from Kainic acid-induced neuronal cell loss in the rat hippocampus"European Journal of Neuroscience. 11. 3617-3625 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. OCHIISHI, Y. SAITOH, A. YUKAWA M. SAJI, Y. REN, T.SHIRAO, H. MIYAMOTO, H. HAKATA Y.SEKINO: "HIGH LEVEL OF ADENOSITE A1 RECEPTOR-LIKE IMMUNOREACTIVITY IN THE CA 2/ CA3a REGION OF THE ADULT RAT HIPPOCAMPUS"Neuroscience. 93.3. 955-967 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] YUKO SEKINO, KUNIHIRO OBATA, MANABU TANIFUJI, MAKOTO MIZUNO, JIN MURAYAMA: "Delayed Signal Propagation via CA2 in Rat Hippocampal Slices Revealed by Optical Recording"The American Physiological Society. 1662-1668 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Saotoshi Fujii, Yuko Sekino, Yoichiro Kuroda, Hiroshi Sasaki, Ken-Ichi Ito, Hiroshi Kato: "8-Cyciopentyltheophilline an adenosine A receptor antagonise inhibits the reversal of long-term potentiation in hippocampal CA1 Newrons"European Journal of Pharmacology. 331. 9-14 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ochiishi.T.et al Sekino,Y(CA): "High Level of Adenosine A_1 Receptor-like Immunoreactivity in the CA2/CA3 a Region at the Adult Rat Hippocimpus." Neuroscience. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shirao.T,Y.Ren R.Hayashi & Y.Sekino: "Time-lapse Video Microscopic Analysis of cell process Formation from Drebrin A-Expressing Fibroblast" Keio University Symposia for Life Science and Medicine. 2. 381-385 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sekino,Y.et al: "Delayed Signal propagation via CA2 in Rat Hippocompal Slices Revealed by Optical Recording" J.Neurophysiol. 78. 1662-1668 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fujii,S.,Y.Sekino,et al: "8・Cyclopentyltheophylline,an Adenosine A1 Receptor Antagonist,Inhibits the Revearsal of Long-term Potentiation in Hippocompal CA1 Neurons" Eur.J Pharmacol.331. 9-14 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 白尾智明 関野祐子: "脳と神経 分子神経生物科学入門" 金子章道、川村弘毅、植村應一(共立出版), 360 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 吉岡享、関野祐子: "イオンチャネル電気信号をつくる分子" 曽我部正博 (共立出版), 224 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yuko Sekino: "Delayed Signal Propagation Uia CA2 in Rat Hippocampalslicos Reuedled by Opfical Recording" Journal of Neurophysiology. 78. 1662-1668 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Satoshi Fujii: "8-Cyclopentyl the ophylline an adenosine A1 veceptor antagonst inhibits the reversal of long-term potentiation in hippocanpal CA1 neurons" European Journal of Pharmacology. 331. 9-14 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 関野祐子(分担): "シリーズ・ニューバイオフィジックス(5)イオン チャネル" 日本生物物理学会シリーズ・ニューバイオフィジックス刊行委員会, 224 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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