• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification and analysis of the genes that regurate the bahavior of the honeybee

Research Project

Project/Area Number 09480228
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionThe University of Tokyo

Principal Investigator

KUBO Takeo  The University of Tokyo, Graduate School of Pharmaceutical Sciences, Associate Professor, 大学院・薬学系研究科, 助教授 (10201469)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 1998: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1997: ¥9,600,000 (Direct Cost: ¥9,600,000)
KeywordsInsect / Social insect / Honeybee / Behavior / memory & learning / Mushroom body / cDNA / Calcium / 記憶 / Ca^<2+> / IP3受容体 / cDNAクローニング / RNA / 膜翅目
Research Abstract

Honeybee is a social insect and many exquisite communications are performed among the colony members such as "dance launguage". However, the molecular basis that underlies such highly advanced types of honeybee behavior is largely unknown. The mushroom bodies (MB), which are supposed to be a main center of the senssory integration and memory, are characteristically well-developed in comparison with those of other insects. Thus, the increased complexity of the function and structure of the MB might be due to the highly advanced types of honeybee bahavior. Here, we searched for genes that are expressed preferentially in the MB by the differential display method as candidate genes that regulate the honeybee behavior, and obtained the following results.
1. The intrinsic ceuron cells that consist the MB are subdivided into two classes : the large-and small-type Kenyon cells. A gene which is expressed specifically in the large-type Kenyon cells, termed M5, and a gene which is expressed specif … More ically in the small-type Kenyon cells, termed Ks, are identified for the first time. cDNAs for these genes did not contain significant long open reading frames, suggesting that the M5 and Ks gene transcripts function as non-translated RNAs. In situ hybridization analysis of the M5 gene transcripts in the pupal brain revealed that the honeybee MB is a quadruple structure consisting of four clonal units, each of wich contains two distinct areas that express different genes. Furthure analysis of the functions of these gene products will reveal the subdivision of the inner structure of the MB that uinderlie the honeybee behavior.
2. We showed that the expression of the genes for proteins involved in the intracellular calcium signal transduction was up-regulated in the MB of the honeybee. In contrast, in Drosophila, these genes were reported to be expressed ubiquitously thoroughout the brain cortex. Calcium signal transduction system of the neural cell has been reported to be involved in the estabrishment of memory and learning. Thus, it seems possible that the enhancement of the function of the calcium signaling contributes to the enhanced sinaptic plasticity of the MB of the honeybee. These findinG will be inportant from the perspective of comparative neurobiology of the insect. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kamikouchi,A: "Preferential expression of the gene for a putative inositol 1,4,5-triphosphate receptor homologue in the mushroom bodies of the brain of the worker honeybee Apis mellifera L." Biochem.Biophys.Res.Commun.242. 181-186 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohashi,K: "Change in the mode of gene expression of the hypopharyngeal gland cells with an age-dependent role change of the worker honeybee Apis mellifera L." Eur.J.Biochem.249. 797-802 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimoaraiso,M: "Identification of the region in yeast S-II that defines species specificity in its interaction with RNA polymerase II." J.Biol.Chem.272. 26550-26554 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sekizawa,Y.: "Molecular cloning of cDNA for lysenin, a novel protein in the earthworm Eisenia foetida that causes contraction of rat vascular smooth muscle." Gene. 191. 97-102 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Arai,T: "Cloning of cDNA for regenectin, a humoral C-type lectin of Periplaneta americana, and expression of the regenectin gene during leg regeneration." Insect Biochem.Mol.Biol.28. 987-994 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kitabayashi,N.A.: "Molecular cloning of cDNA for p10, a novel protein that increases in the regenerating legs of Periplaneta americana(American cockroach)" Insect Biochem.Mol.Biol.28. 785-790 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ito,T.: "Gene organization and chromosome mapping of the testis-specific S-II." Mammalian Genome. 9. 915-917 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Taira,Y.: "Molecular cloning of cDNA and tissue-specific expression of the gene for SII-K1,a novel transcription elongation factor SII" Genes to Cells. 3. 289-296 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kamikouchi, A.et al.: "Preferential expression of the gene for a putative inositol 1,4,5-triphosphate receptor homologue in the mushroom bodies of the brain of the worker honeybee Apis mellifera L." Biochem.Biophys.Res.Commun.242. 181-186 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ohashi, K.et al.: "Change in the mode of gene expression of the hypopharyngeal gland cells with an age-dependent role change of the worker honeybee Apis mellifera L." Eur.J.Biochem.249. 797-802 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimoaraiso, M.et al.: "Identification of the region in yeast S-II that defines species specificity in its interaction with RNA polymerase II." J.Biol.Chem.272. 26550-26554 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sekizawa, Y.et al.: "Molecular cloning of cDNA for lysenin, a novel protein in the earthworm Eisenia foetida that causes contraction of rat vascular smooth muscle." Gene. 191. 97-102 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Arai, T.et al.: "Cloning of cDNA for regenectin, a humoral C-type lectin of Periplaneta americana, and expression of the regenectin gene during leg regeneration." Insect Biochem.Mol.Biol.28. 987-994 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kitabayashi, N.A.et al.: "Molecular cloning of cDNA for p10, a novel protein that increases in the regenerating legs of Periplaneta americana (American cockroach)" Insect Biochem.Mol.Biol.28. 785-590 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Oti, T.et al.: "Gene organization and chromosome mapping of the testis-specific S-II." Mammalian Genome. 9. 915-917 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Taira, Y.et al.: "Molecular cloning of cDNA and tissue-specific expression of the gene for SII-K1, a novel transcription elongation factor SII" Gene Cell.3. 289-296 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kamikouchi,A: "Preferential expression of the gene for a putative inositol 1,4,5-triphosphate receptor homologue in the mushroom bodies of the brain of the worker honeybee Apis mellifera L." Biochem.Biophys.Res.Commun.242. 181-186 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Arai,T: "Cloning of cDNA for regenectin,a humoral C-type lectin of Periplaneta americana,and expression of the regenectin gene during leg regeneration." Insect Biochem.Mol.Biol.28. 987-994 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitabayashi,N.A.: "Molecular cloning of cDNA for p10,a novel protein that increases in the regenerating legs of Periplaneta amcricana(American cockroach)" Insect Biochem.Mol.Biol.28. 785-790 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ito,T.: "Gene organization and chromosome mapping of the testis-specific S-II." Mammalian Genome. 9. 915-917 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kamikouchi,A: "Preferential expression of the gene for a putative inositol 1,4,5-triphosphate receptor homologue in the mushroom bodies of the brain of the worker honeybee Apis mellifera L." Biochem.Biophys.Res.Commun.242. 181-186 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohashi,K: "Change in the mode of gene expression of the hypopharyngeal gland cells with an age-dependent role change of the worker honeybee Apis mellifera L." Eur.J.Biochem.249. 797-802 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shimoaraiso,M: "Identification of the region in yeast S-II that defines species specificity in its interaction with RNA polymerase II." J.Biol.Chem.272. 26550-26554 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sekizawa,Y.: "Molecular cloning of cDNA for lysenin,a novel protein in the earthworm Eisenia foetida that causes contraction of rat vascular smooth muscle." Gene. 191. 97-102 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi