| Project/Area Number |
09555120
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
計測・制御工学
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
SHIBATA Masahiro UNIV OF TOKYO,GRAD SCH OF MED ASSIST PROF, 大学院・医学系研究科, 講師 (60158954)
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| Co-Investigator(Kenkyū-buntansha) |
SHIO Megumu NIKON ENGINEERING CO DIRECTOR, 研究開発部, 部長(研究職)
KAMIYA Akira UNIV OF TOKYO,GRAD SCH OF MED PROF, 大学院・医学系研究科, 教授 (50014072)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1997: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | OXYGEN TENSION / VITAL MICROSCOPY / LASER / MICROCIRCULATION / Pd-PORPHYRIN / SKELETAL MUSCLE / Pdポルフィリン / リン光寿命 / Stern-Volmer |
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| Research Abstract |
To clarify the oxygen transport across the microvessel and oxygen distribution in the skeletal muscle, we have newly designed an intravital laser microscope with oxygen-dependent quenching of phosphorescence technique. The phosphorescence probe for oxygen was injected into the systemic blood, and phosphorescence excited by N_2/dye pulse laserin the tissue area of 15 mum in diameter was measured by a photomultiplier. In vitro and in vivo oxygen tension (pO2) measurements revealed the efficiency of this method because of its non-invasiveness, accuracy, and rapid response time. This technique was applied to pO2 measurements in the rat microcirculation. The arteriolar and venular pO2 measurements were performed at the several regions classified by branching order. The mean arteriolar pO2 of different orders decreased according to increase the branching(A1 : 72.4, A2 : 51.8 and A3 : 44.1 mmHg), however venular pO2 were independent on vessel orders(V1 : 27.5, V2 : 28.6 and V3 : 32.0 mmHg). Interstitial pO2 adjacent to arterioles of different orders were significantly lower than those of intravascular pO2 (I1 : 47.8, I2 : 35.8 and I3 : 24.4 mmHg). In conclusion our new designed intravital microscope was proved the effective method to clarify the mechanisms of oxygen transport across the microvessels and the oxygen distribution in the skeletal muscle. In addition, the existence of significant reduction of pO2 levels in arterioles of different orders and large pO2 gradients at the interface between blood and tissue suggest that oxygen consumption of the smooth muscle and/or endothelium in the skeletal muscle arteriole might be high.
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