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DEVELOPMENT OF SLIT LASER MICROSCOPE FOR QUANTIFICATION OF TISSUE PO2 DISTRIBUTION

Research Project

Project/Area Number 09555120
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 計測・制御工学
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

SHIBATA Masahiro  UNIV OF TOKYO,GRAD SCH OF MED ASSIST PROF, 大学院・医学系研究科, 講師 (60158954)

Co-Investigator(Kenkyū-buntansha) SHIO Megumu  NIKON ENGINEERING CO DIRECTOR, 研究開発部, 部長(研究職)
KAMIYA Akira  UNIV OF TOKYO,GRAD SCH OF MED PROF, 大学院・医学系研究科, 教授 (50014072)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1997: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsOXYGEN TENSION / VITAL MICROSCOPY / LASER / MICROCIRCULATION / Pd-PORPHYRIN / SKELETAL MUSCLE / Pdポルフィリン / リン光寿命 / Stern-Volmer
Research Abstract

To clarify the oxygen transport across the microvessel and oxygen distribution in the skeletal muscle, we have newly designed an intravital laser microscope with oxygen-dependent quenching of phosphorescence technique. The phosphorescence probe for oxygen was injected into the systemic blood, and phosphorescence excited by N_2/dye pulse laserin the tissue area of 15 mum in diameter was measured by a photomultiplier. In vitro and in vivo oxygen tension (pO2) measurements revealed the efficiency of this method because of its non-invasiveness, accuracy, and rapid response time. This technique was applied to pO2 measurements in the rat microcirculation. The arteriolar and venular pO2 measurements were performed at the several regions classified by branching order. The mean arteriolar pO2 of different orders decreased according to increase the branching(A1 : 72.4, A2 : 51.8 and A3 : 44.1 mmHg), however venular pO2 were independent on vessel orders(V1 : 27.5, V2 : 28.6 and V3 : 32.0 mmHg). Interstitial pO2 adjacent to arterioles of different orders were significantly lower than those of intravascular pO2 (I1 : 47.8, I2 : 35.8 and I3 : 24.4 mmHg). In conclusion our new designed intravital microscope was proved the effective method to clarify the mechanisms of oxygen transport across the microvessels and the oxygen distribution in the skeletal muscle. In addition, the existence of significant reduction of pO2 levels in arterioles of different orders and large pO2 gradients at the interface between blood and tissue suggest that oxygen consumption of the smooth muscle and/or endothelium in the skeletal muscle arteriole might be high.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] M.Shibata,S.Ichioka,A.Kamiya: "Dual-beam laser illuminator of fluorescence microscope for in vivo microcirculation studies." Med.& Biol. Eng. & Comput.37. in press (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shibata,A.Kamiya: "A phosphorescence measurement of tissue oxygen distribution in the rat cremaster muscle." Proc.Eur.Microcirc.177-182 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Ichioka,M.Shibata,K.Kosaki Y.Sato, K.Harii, A.Kamiya: "In vivo measurement of morphometric hemodynamic changes in the microcirculation during angiogenesis under chronic α1-adrenergic blocker treatment." Microvasc.Res.55. 165-174 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Takahashi,M.Shibata,A.Kamiya: "Mechanism of macromolecule concentration in collecting lymphatics in rat mesentery." Microvasc.Res.54. 193-205 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Satake,M.Shibata,S.Shibata: "Endothelium-and cytochrome P-450-dependent rela xation induced by isoproterenol in rat aortic rings." Eur.J.Pharmacol.319. 37-41 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Satake,M.Shibata,S.Shibata: "The involvement of K_<Ca>,K_<ATP> and K_v channels in vasorelaxing responses to acetylcholine in rat aortic rings." Gen.Pharmacol.28. 453-457 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shibata, S.Ichioka, A.Kamiya: "Dual-beam laser illuminator of fluorescence microscope for in vivo microcirculation studies." Med.& Biol.Eng.& Comput.37 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shibata, A.Kamiya: "A phosphorescence measurement of tissue oxygen distribution in the rat cremaster muscle." Proc.Eur.Microcirc.177-182 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Ichioka, M.Shibata, K.Kosaki, Y.Sato, K.Harii, A.Kamiya: "In vivo measurement of morphometric hemodynamic changes in the microcirculation during angiogenesis under chronic al-adrenergic blocker treatment." Microvasc.Res.55. 165-174 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Ichioka, M.Iwasaka, M.Shibata, K.Harii, A.Kamiya, S.Ueno: "Biological effects of magnetic fields on the microcirculatory blood flow in vivo : A preliminary report." Med.& Biol.Eng.& Comput.36. 91-95 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Takahasih, M.Shibata, A.Kamiya: "Mechanism of macromolecule concentration in collecting lymphatics in rat mesentery." Microvasc.Res.54. 193-205 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Satake, M.Shibata, S.Shibata: "Endothelium-and cytochrome P-450-dependent relaxation induced by isoproterenol in rat aortic rings." Eur.J.Pharmacol.319. 37-41 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Satake, M.Shibata, S.Shibata: "The involvement of K_<Ca'> K_<ATP> and K_V channels in vasorelaxing responses to acetylcholine in rat aortic rings." Gen.Pharmacol.28. 453-457 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Ichioka, M.Shibata, K.Kosaki, Y.Sato, K.Harii, A.Kamiya: "Effect of shear stress on wound-healing angiogenesis in the rabbit ear chamber." J.Surg.Res.72. 29-35 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Shibata,S.Ichioka,A.Kamiya: "Dual-beam laser illuminator of fluorescence microscope for in vivo microcirculation studies." Med.& Biol.Eng.& Comput.37(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Shibata,A.Kamiya: "A phosphorescence measurement of tissue oxygen distribution in the rat cremaster muscle." Proc.Eur.Microcirc.177-182 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Ichioka,M.Shibata,K.Kosaki,Y.Sato,K.Harii,A.Kamiya: "In vivo measurement of morphometric hemodynamic changes in the microcirculation during angiogenesis under chronic α1-adrenergic blocker treatment." Microvasc.Res.55. 165-174 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Takahashi,M.Shibata,A.Kamiya: "Mechanism of macromolecule concentration in collecting lymphatics in rat mesentery." Microvasc.Res.54. 193-205 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.Satake,M.Shibata,S.Shibata: "Endothelium- and cytochrome P-450-dependent relaxation induced by isoproterenol in rat aortic rings." Eur.J.Pharmacol.319. 37-41 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.Satake,M.Shibata,S.Shibata: "The involvement of K_<Ca>,K_<ATP> and K_ν channels in vasorelaxing responses to acetylcholine in rat aortic rings." Gen.Pharmacol.28. 453-457 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Takahashi: "Mechanism of macromolecule concentration in collection lymphatics in rat mesentery." Microvasc.Res.54. 193-205 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Ichioka: "Effects of shear stress on wound-healing angiogenesis in the rabbit ear chamber." J.Surg.Res.72. 29-35 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Satake: "Endothelium-and cytochrome P-450-dependent relaxation induced by isoproterenol in rat aortic rings." Eur.J.Pharmcol.319. 37-41 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Satake: "The Involvement of Kc_a,K_<ATP> and K_V channels in vasorelaxing responses to acetylcholine in rat aortic rings." Gen.Pharmac.28-3. 453-457 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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