| Project/Area Number |
09555241
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
反応・分離工学
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| Research Institution | Seikei University |
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Principal Investigator |
HIGUCHI Akon Seikei University, Faculty of Engineering, Professor, 工学部, 教授 (30189766)
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Tetsuo Asahi Chemical.Ind.Co., BMM Development Institute, Principal Scientist, BMM開発研究所, 室長
SATOH Sakae Asahi Chemical.Ind.Co., BMM Development Institute, Director, BMM開発研究所, 所長
MANABE Sei-ichi Fukuoka Women's University, Faculty of Human Environmental Science, Professor, 人間環境学部, 教授 (50265013)
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| Project Period (FY) |
1997 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥10,900,000 (Direct Cost: ¥10,900,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1997: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | Membrane / DNA / Virus / Chromatin / Virus removal / Porous Membrane |
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| Research Abstract |
Concentration and purification of Virus and DNA from biopharmaceuticals was investigated. The followings are specifically clarified ;
1. The permeation of histone-DNA and chromatin through regenerated cellulose hollow fibers having a mean pore diameter of 15 nm was substantially lower than the permeation of free DNA.
2. When γ-globulin solution permeated through the virus removal membranes having a mean pore diameter of 35 nm and 15 nm, a drastic increase in flux was found with a decrease in DNA in the feed solution, which was normally contained in the protein solution as a contamination in the order of ppb. A DNA-γ-globulin complex having a particle size of 135-225 nm was detected by dynamic light scattering measurements in the γ-globulin solution.
3. The DNA-γ-globulin complex was destroyed by the addition of 0.4 mol/l NaCl in the γ-globulin solution. The flux of the γ-globulin solution through the membranes drastically increased with the increase in NaCl concentration up to 0.1 mol/l. This is explained by a decrease in the DNA-γ-globulin complex in the presence of NaCl in the γ-globulin solution. The effect of flux on the concentration of NaCl was found to depend on the pore sizes and structure of the membranes and membrane materials.
4. The DNA-γ-globulin complex was also destroyed by the treatment of DNAase in the γ-globulin solution. The flux of the γ-globulin solution through the membranes drastically increased when the biodrug solution was treated with DNAase. This is explained by a decrease in the DNA-γ-globulin complex from segmentation of DNA in the DNA-γ-globulin complex containing the γ-globulin solution.
5. It is concluded that the DNA molecule and virus could diffuse into the smaller pores in the regenerated cellulose solid than the size of the molecule by the deformation of the shape into a thread-like shape.
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