| Project/Area Number |
09555249
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
生物・生体工学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SEKI Minoru Dept. Chem. Biotechnol., Univ. of Tokyo, Associate Professor, 大学院・工学系研究科, 助教授 (80206622)
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| Co-Investigator(Kenkyū-buntansha) |
FURUSAKI Shintaro Dept. Chem. Systems & Eng., Kyushu Univ., Professor, 大学院・工学研究科, 教授 (40011209)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | Plant Cell Culture / Taxus sp. / Taxol / Paclitaxel / Anti-Cancer Drug / Immobilization / Bioreactor / Bioprocess Engineering / タキソ-ル / 培地循環 |
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| Research Abstract |
Medium recycling as an operational strategy to increase secondary metabolite formation in plant cell culture was investigated for an anti-cancer drug, paclitaxel (Taxol【○!R.】) production using cultured cells of three Taxus spp. Using a continuous production system, paclitaxel productivity was increased by a factor of ten compared to batch operation. The continuous production of paclitaxel by immobilized cells was successfully continued for up to 40 days. A novel paclitaxel production process with medium recycling using plant cell culture of Taxus spp. is proposed. For paclitaxel recovery, the extraction with organic solvent and the adsorption with hydrophobic resins were coupled to the medium-recycling perfusion-culture system for paclitaxel production, which increased the paclitaxel productivity.
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