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Hatching of avian embryos under artificial environment and establishment of transgenic chicken

Research Project

Project/Area Number 09555251
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 生物・生体工学
Research InstitutionGraduate School of Engineering, Nagoya University

Principal Investigator

IIJIMA Shinji  Prof.Dept.of Biotechnol.Grad.School of Eng., Nagoya Univ., 大学院・工学研究科, 教授 (00168056)

Co-Investigator(Kenkyū-buntansha) 穴沢 秀治  協和発酵工業(株), 東京研究所, 主任研究員
KAMIHIRA M.  Assoc.Prof.Dept.of Biotechnol.Grad.School of Eng., Nagoya Univ., 大学院・工学研究科, 助教授 (40202022)
ANAZAWA H.  Investigator, Tokyo Research Institute, Kyowa Hakkou.Co.
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 1998: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1997: ¥9,200,000 (Direct Cost: ¥9,200,000)
KeywordsQuail / Chicken / Embryo / DNA transfection / Ovalbumm / Transgenic Chicken / 孵化
Research Abstract

This year, we studied the conditions for gene transfection to embryos. For this, we applied liposome method for embryos of various developmental stages. DNA and liposome complex was injected from heat to blood vessel. Embryos after 48h fertilization gave both the highest survival and transfection efficiency. In this experiment, only heart cells showed higher expression of the introduced gene.
Then we improved this point by using protamine. For this hydrophobic residues were coupled to promine and liposome protamine complex was stabilized. By using this modified liposome, the area which showed the expression of introduced DNA was expanded.
On the other hand, we developed the vector system to achieve oviduct spcific gene expression. For this we cloned ovalbimin genes from chicken fibroblast DNA.From 100,000 recombinant phages, we isolated a phage containing ovalbumin promotor region. Human insulin gene DNA was ligated to the promoter region to construct the expression vector.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Masamichi Kamihira,Satoshi Oguchi,Ayuko Tachibana,Yuki Kitagawa and Shinji Iijima: "Improved hatching for in vitro quail embryo culture using sarrogate eggshell and artificial vessel" Development Growth and Differentiation. Vol.40. 449-455 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Jun You,Masamichi Kamihira and Shinji Iijima: "Enhancement of transfection efficiency using ligand-modified lipid vesicles" Journal of Fermentation and Bioengineering. Vol.85(5). 525-528 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masamichi Kamihira, Satoshi Oguchi, Ayuko Tachibana, Yuki Kitagawa and Shinji Iijima: "Improved hatching for in vitro quail embryo culture using sarrogate eggshell and arti-ficial vessel" Development Growth and Differentiation. Vol.40. 449-455 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Jun You, Masamichi Kamihira and Shinji Iijima: "Enhancement of transfection efficiency using ligand-modified lipid vesicles" Journal of Fermentation and Bioengineering. Vol.85(5). 525-528 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masamichi Kamihira,Satoshi Oguchi,Ayuko Tachibana,Yuki Kitagawa and Shinji Iijima: "Improved hatching for in vitro quail embryo culture using sarrogate eggshell and arti-ficial vessel" Development Growth and Differentiation. Vol.40. 449-455 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Jun You,Masamichi Kamihira and Shinji Iijima: "Enhancement of transfection efficiency using ligand-modified lipid vesicles" Journal of Fermentation and Bioengineering. Vol.85(5). 525-528 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 上平正道、飯島信司: "人工環境下における鳥類胚培養" バイオサイエンスとインダストリー. 55・2. 104-106 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masamichi Kamihira, Jun You and Shinji Iijima: "Surfactant-mediated gene transfer for mammalian cells" Animal Cell Technology. 8. 381-385 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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