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Identification of molecular markers linked to natural astringency loss of persimmon

Research Project

Project/Area Number 09556008
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field 園芸・造園学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YONEMORI Keizo  Grad.Sck.Agr., KYOTO UNIVERSITY, Associate Professor, 農学研究科, 助教授 (10111949)

Co-Investigator(Kenkyū-buntansha) TETSUMURA Takuya  Grad.Sck.Agr., KYOTO UNIVERSITY, Assistant Professor, 農学研究科, 助手 (00227498)
TAO Ryutaro  Grad.Sck.Agr., KYOTO UNIVERSITY, Lecturer, 農学研究科, 講師 (10211997)
YAMADA Masuhiko  Per.Grape.Res.Center, NIFTS, Researcher, カキ・ブドウ支場, 研究室長(研究職)
KANZAKI Shinya  Fac.Agr., Kinki Univ., Assistant Prof., 農学部, 助手 (20330243)
SATO Akihiko  Per.Grape.Res.Center, NIFTS, Researcher, カキ・ブドウ支場, 研究員(研究職)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1997: ¥7,200,000 (Direct Cost: ¥7,200,000)
KeywordsJapanese persimmon / natural astringency loss / molecular marker / PCNA / RFLP / AFLP / 日渋性マーカー / 脱渋性 / ゲノム構成 / 甘渋判定マーカー
Research Abstract

The pollination-constant and non-astringent (PCNA)-type fruit is the most desirable persimmon for fresh consumption, and new PCNA-type cultivars with good fruit quality have been awaited worldwide. However, the inheritance of PCNA-type is qualitative and the PCNA-type is recessive to non-PCNA (PVNA, PVA, and PCA) types. Molecular marker closely linked to the trait of natural astringency-loss is a powerful tool for the breeding project. So, we tried to establish it by the following experiments to make the breeding process more efficient.
1. Bulked segregant analysis (BSA) of 6 PCNA-type and 10 non-PCNA-type offspring in breeding population was performed by AFLP method using 128 primer combinations. As a result, 4 potential AFLP markers were detected for discriminating PCNA- and non-PCNA types.
2. The most reliable marker among 4 potential AFLP markers was selected and sequenced. When this marker was used as a probe for Southern analysis after genomic DNA was digested HindlII, all progenies examined could be distinguished between PCNA-type and non-PCNA type by the existence or absence of 6.5kb or 8.0 kb band. Furthermore, segregation rate of these bands indicated that persimmon shows polysomic inheritance. Persimmon seemed to be an auto- or autoallo-hexaploid.
3. When Southern analysis using AFLP maker as a probe was examined to existing cultivars, PCNA cultivars could be distinguished from non-PCNA culitvars with 100% accuracy. This result indicates a high reliability of this analysis for selecting PCNA-type among wider breeding populations.
4. The sequencing analysis of two RFLP markers (6.5 kb and 8.0 kb) for characterizing them and making primers for PCR analysis for easier detection of these makers was not completely successful. We could sequence 8.0 kb band but not 6.5 kb band. So, further study was needed to design PCR primers for more easier method than Southern analysis to distinguish PCNA-type from non-PCNA-type.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] S.Kanzaki,K.Yonemori,A.Sugiura,A.Sato,M.Yamada: "Identification of molecular makers linked to the trait of natural astringency loss of Japanese persimmon (Diospyros kaki) fruit."J.Amer.Soc.Hort.Sci.. 126. 51-55 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Kanzaki,K.Yonemori,A.Sugiura,A.Sato,M.Yamada: "Evaluation of RFLP analysis for discriminating PCNA genotype in some persimmon cultivars."J.Japan.Soc.Hort.Sci.. 69. 702-704 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Kanzaki,K.Yonemori,A.Sugiura,A.Sato,M.Yamada: "Analysis of the genetic relationships among pollination-constant and non-astringent (PCNA) cultivars of persimmon from Japan and China using AFLP."J.Japan.Soc.Hort.Sci.. 69. 665-670 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Kanzaki, K.Yonemori, A.Sugiura, A.Sato, M.Yamada: "Identification of melecular markers linked to the trait of natural astringency loss of Japanese persimmon (Diospyres kaki) fruit."J.Amer.Soc.Hort.Sci.. 126. 51-55 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Kanzai, K.Yonemori, A.Sugiura, A.Sato, M.Yamada: "Evaluation of RFLP analysis for discriminating PCNA genotype in some persimmon caltivars."J.Japan.Soc.Hort.Sci.. 69. 702-704 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Kanzaki, K.Yonemori, A.Sugiura, A.Sato, M.Yamada: "Analysis of the genetic relationships among pollination-coustant and non-astringent (PCNA) cultivars of persimmon from Japan and China using AFLP."J.Japan.Soc.Hort.Sci.. 69. 665-670 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Kanzaki,K.Yonemori.A.Sigiura,A.Sato,M.Yamado: "Analysis of the genetic relationships among polliantion-constant and non-astringent cultivars of persimmon from Japan and China using AFLP."J.Japan.Soc.Hort.Sci.. 69・6. 665-670 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.kanzaki,K.Yonemori,A.Singiura,A.Sato,M.Yamada: "Identification of molecular markers linked to the trait of natural astringency loss of Japanese persimmon (Diospyros kaki) fruit."J.Amaer.Soc.Hort.Sci.. 126・1. 51-55 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Kanzaki,K.Yonemori,A.Sato,M.Yamada,A.Sugiura: "RFLP analysis for distinguishing the trait of natural astringency-loss in 33 persimmon (Diospyros Kaki Thunb.) cultivars"Abstract for Plant & Animal Genome VIII. vol.8. 135-135 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 神崎真哉,米森敬三,佐藤明彦,山田昌彦,杉浦明: "完全甘ガキ選抜のために同定したRFLPマーカーの品種および交雑実生における有効性"園芸学会雑誌. 69 (別1) (発表予定). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.Kanzaki,K.Yonemori,A.Sato,M.Yamada,A.Sugiura: "Effectiveness of RFLP analysis for discriminating PCNA genotype in some persimmon cultivars"Journal of the Japanese Society for Haticultural Science. vol.69 (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.Kanzaki,K.Yonemori,M.Yamada,A.Sato,A.Sugiura: "Identification of an AFLP-marker linked to the locus controlling the trait of astringency in Japanese persimnron (Diospyros kaki Thunb.)." Abstract for plant & Animal Genome VII. vol.7. 128-128 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 神崎真哉,米森敬三,山田昌彦,佐藤明彦,杉浦 明: "完全甘ガキの早期選抜のための分子マーカーの同定" 園芸学会雑誌. 68(別). 132-132 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Kanzaki, K.Yonemori, M.Yamada, A.Sato, A.Sugiura: "An analysis of genetic variation in PCNA type cultivars of Japanese persimmon (Diospyros kaki Thunb.) using AFLP markers." Journal of the Japanese Society for Horticultural Science. 67(suppl.2)(発表予定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Kanzaki, K.Yonemori, M.Yamada, A.Sato, A.Sugiura: "Survey of specific marker for PCNA (pollination-constant and non-astringent) nature of Japanese persimmon (Diospyros kaki Thunb.) by AFLP analysis." Abstract for XXVth International Horticultural Congress. (発表予定).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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