| Project/Area Number |
09556013
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Utsunomiya University |
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Principal Investigator |
HIRAI Hideaki (1998-1999) Utsunomiya Univ., Faculty of Agriculture, Associate Professor, 農学部, 助教授 (20208804)
関本 均 (1997) 宇都宮大学, 農学部, 助教授 (10261819)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Takahiro Utsunomiya Univ., Faculty of Agriculture, Associate Professor, 農学部, 助教授 (50221990)
ICHIZEN Nobumasa Utsunomiya Univ., Center for Research of Wild Plants, Professor, 野生植物科学研究センタ, 教授 (50008067)
伊谷 樹一 宇都宮大学, 農学部, 講師 (20232382)
平井 英明 宇都宮大学, 農学部, 助教授 (20208804)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Rhizosphere / Image / Image Analysis / Root box / pH / Nitrate / Phosphorus / Root Activity / 水稲 / 画像分類 / 根圏環境 / 元素組成 / 根圏pH / 発砲樹脂 |
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| Research Abstract |
We have established an observation method of rhizosphere to reflect the nutritional conditions using by both a new plant root box made of phenolic resin form for developing whole root systems in water culture and image analysis. The root box allowed better visualization of changes in pH and the distribution of nitrate and phosphorus by image analysis in a representative whole root system. The pH values were detected with pH indicators, nitrate and phosphorus concentration with color reagents, sprayed onto profiles of the root system.
We observed acidification of the medium in the root system of wheat grown 1/4(NHィイD24ィエD2-N / NOィイD23ィエD2-N) medium until 12h, while at 48h the pH rose again to 6.0 and higher. This rise was accompanied by a decrease in the nitrate concentration at 24 and 48h. In contrast, the pH increased around the roots of chinese cabbage with time, while nitrate decreased, suggesting relationship between rhizosphere pH changes and preferential cation / anion uptake of plants. Also we observed the acidification of rhizosphere with iron and phosphorus deficiency, and by coexisting insoluble phosphate salts (AlPOィイD24ィエD2 and FePOィイD24ィエD2).
In addition, we evaluated the root activity under the different fertilized conditions by image analysis of root color in stead of the judgment by eyes.
The method we established can be applied to the nutritional diagnosis of plants.
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