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Construction of a new improvement system using DNA molecular technique in the forest tree species

Research Project

Project/Area Number 09556032
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 林学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

SHIRAISHI Susumu  Faculty of Agriculture, KYUSHU UNIVERSITY Professor, 大学院・農学研究院, 教授 (70226314)

Co-Investigator(Kenkyū-buntansha) KURINOBU Susumu  Forest Tree Breeding Center, Kyushu Regional Breeding Office, Head of Research Division, 九州育種場, 研究員
TACHIKAWA Hiroshi  Nippon Paper Industries Co., Ltd., Research Laboratory of Chemical Development, Researcher, 化成品開発研究所, 研究員
SHIBATA Masaru  Oji Paper Co., Ltd., Forestry Research Institute, Director, 森林資源研究所, 研究員
MATSUMURA Junji  Faculty of Agriculture, KYUSHU UNIVERSITY Assistant Professor, 大学院・農学研究院, 助手 (70243946)
TAKATA Katsuhiko  Faculty of Agriculture, KYUSHU UNIVERSITY Assistant Professor, 大学院・農学研究院, 助手 (50264099)
ODA Kazuyuki  Faculty of Agriculture, KYUSHU UNIVERSITY Professor, 大学院・農学研究院, 教授 (10045130)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 1999: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1997: ¥7,200,000 (Direct Cost: ¥7,200,000)
KeywordsDNA molecular marker / breeding system / cultivar diagnosis / clone analysis / parentage diagnosis / MuPS analysis / chloroplast DNA marker / SCAR / 林木育種 / 品種識別 / 育種戦略 / 父性遺伝
Research Abstract

For constructing an effective improvement system of forest tree species using DNA molecular technique, we developed a new DNA molecular marker and tried to apply the actual breeding.
(1) A new DNA molecular marker for identification of cultivars/clones : In Acacia mangium and Cryptomeria japonica (Sugi), more than 18 SCAR (sequence characterized amplified regions) markers were designed from RAPD (random amplified polymorphic DNA) fragments in each species. By combining these SCAR markers, a MuPS (multiplex-PCR of SCAR markers) system was constructed using Multiplex-PCR.This system possesses the discrimination power to identify ca. 260,000 genotypes.
(2) Survey of the intra-specific chloroplast DNA polymorphism in Pinus thunbergii : BESS (base excision sequence scanning), CFLP (cleavase fragment length polymorphism), and SSR (simple sequence repeats) analysis of the chloroplast DNA revealed the eight mutations in the intergenic spacer regions. These chloroplast DNA markers were useful for ascent the pollen parent of progenies.
(3) Application MuPS system into the classification of Sugi cultivars and plus tree clones : We determined the MuPS types of 77 cultivars and 589 plus tree clone of Kyushu and constructed the data base of their MuPS types. As the result of MuPS analysis, quite a few plus tree clones originated from some cultivars or some unknown cutting clones. These duplicated clones should be eliminated from the plus trees population of Kyushu region.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report

Research Products

(37 results)

All Other

All Publications (37 results)

  • [Publications] 宮原文彦: "マツノザイセンチュウ抵抗性クロマツ採種園構成クローンとしての小浜ク-24号の評価"日本林学会誌. 80. 233-235 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 白石 進: "林木のDNA分析法(8)--核DNA分析の応用と発展(1)林木育種への貢献-"林木の育種. 193. 37-41 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 久枝和彦: "DNA分子マーカーを用いた簡便なスギ品種の分類・同定法の開発-RAPD-SCARマーカーのマルチローカス分析-"日本林学会九州支部研究論文集. 52. 49-50 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Widyatomoko,A.: "アカシア4種の葉緑体DNA遺伝子間スペーサー領域のシークエンス変異"日本林学会九州支部研究論文集. 52. 51-52 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 林 誠一: "スギの高密度連鎖地図の作成に向けた基礎的研究"日本林学会九州支部研究論文集. 52. 55-56 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 清藤城宏: "山梨県ヒノキ採種園における鰍沢5号の花粉親としての寄与"日本林学会誌. 82. 105-108 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 清藤城宏: "葉緑体DNAマーカーを用いたヒノキにおける選択受精の検討"日本林学会誌. 82. 380-383 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 久枝和彦: "MuPS(Multiplex-PCR of SCAR markers)分析を用いた九州産スギ精英樹の織別 -佐賀・長崎県産スギ精英樹について-"日本林学会九州支部研究論文集. 53. 55-56 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Seido,K.: "Pollen dispersal in Hinoki(Chamaecyparis obtusa)seed orchard detected using a chroloplast DNA marker"J.For.Res.. 6. 7-12 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Seido,K.: "Determination of the Selfing Rate in Hinoki(Chamaecyparis obtusa)Seed Orchard by Using a Chloroplast PCR-SSCP Marker"Silvae Genetica. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiraishi,S.: "Incomplete paternal inheritance of chloroplast DNA recognized in Chamaecyparis obtusa using an intraspecific polymorphism of the trnD-trnY intergenic spacer region"Theor.Appl.Genet.. (印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 白石 進(共著): "集約林業最前線"フォレスト・リサーチ研究所. 246 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 白石 進(共著): "クローン林業はこうしてつくろう"フォレスト・リサーチ研究所. 394 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyahara, F., Maeda, H., Shiraishi, S.: "Contribution of Obama (t)-24 clone to the seed product in the seed orchard of resistance Japanese black pine (Pinus thunbergii) clones against pine wood nematode."J.Jpn.For.Soc.. 80. 233-235 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiraishi, S.: "DNA analysis (8) of forest tree species. -Application of nuclear DNA into forest tree improvement-"Forest Tree Breeding. 193. 37-41 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hisaeda, K., Shiraishi, S.: "Basic study for a simple identification of Sugi (Cryptomeria japonica D.Don) cultivars using DNA molecular markers."Bull.Kyushu Branch Jpn.For.Soc.. 52. 49-50 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Widyatomoko, A., Shiraishi, S.: "Sequence polymorphism in intergenic spacer region of chloroplast DNA in 4 Acacia species."Bull.Kyushu Branch Jpn.For.Soc.. 52. 51-52 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hayashi, S., Maeda, H., Shiraishi, S.: "A basic study on high density map on Cryptomeria japonica D.Don."Bull.Kyushu Branch Jpn.For.Soc.. 52. 55-56 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Seido, K., Maeda, H., Murakami, H., Ida, K., Shiraishi, S.: "Contribution of clones as the pollen parent in a hinoki (Chamaecyparis obtusa) seed orchard estimated using a chloroplast DNA marker, Kajikazawa 5."J.Jpn.For.Soc.. 82. 105-108 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Seido, K., Yamagishi, K., Ida, K., Murakami, H., Shiraishi, S.: "Investigation of selective fertilization in hinoki (Chamaecyparis obtusa) using a chloroplast DNA marker."J.Jpn.For.Soc.. 82. 380-383 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hisaeda, K., Shiraishi, S.: "Discrimination of Sugi (Cryptomeria japonica D.Don) plus tree clones in Saga and Nagasaki prefectures using MuPS (Multiplex-PCR of SCAR markers)"Bull.Kyushu Branch Jpn.For.Soc.. 53. 55-56 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Seido, K., Maeda, H., Shiraishi, S.: "Pollen dispersal in Hinoki (Chamaecyparis obtusa) seed orchard detected using a chroloplast DNA marker."J.For.Res.. 6. 7-12 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Seido, K., Maeda, H., Shiraishi, S.: "Determination of the Selfing Rate in Hinoki(Chamaecyparis obtusa) Seed Orchard by Using a Chloroplast PCR-SSCP Marker."Silvae Genetica. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiraishi, S., Maeda, H., Toda, T., Seido, K., Sasaki, Y.: "Incomplete paternal inheritance of chloroplast DNA recognized in Chamaecyparis obtusa using an intraspecific polymorphism of the trnD-trnY intergenic spacer region."Theor.Appl.Genet.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiraishi, S.: "Advanced Forest Technology"Forest Research Institute, Kyoto. 246 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiraishi, S.: "Management of clonal forest"Forest Research Institute, Kyoto. 394 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 白石 進: "林木のDNA分析法(8)核DNA分析の応用と発展"林木の育種. 193. 37-41 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 久枝和彦: "MuPS(Multiplex-PCR of SCAR markers)分析を用いた九州産スギ精英樹の識別"日本林学会九州支部研究論文集. 53. 55-56 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 白石 進: "集約林業最前線(分担執筆)"フォレスト・リサーチ研究所. 246 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 白石 進: "クローン林業はこうしてつくろう(分担執筆)"フォレスト・リサーチ研究所. 394 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 宮原春彦: "マツノザイセンチュウ抵抗性クロマツ採種園構成クローンとしての小浜ク-24号の評価" 日本林学会誌. 80・3. 233-235 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 久枝和彦: "DNA分子マーカーを用いた簡便なスギ品種の分類・同定法の開発" 日本林学会九州支部研究論文集. 52 印刷中. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] AYPBC Widyatmoko: "アカシア4種の葉緑体DNA遺伝子間スペーサー領域の塩基配列多型" 日本林学会九州支部研究論文集. 52(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 林 誠一: "スギの高密度連鎖地図の作成へ向けた基礎的研究" 日本林学会九州支部研究論文集. 52(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Maeda,Hajime: "An identification of chloroplast DNA haplotypes of Larix kaempferi and L.gmelinii var.japonica using fluorescence-based PCR-SSCP analysis of rbcL gene" J.Forest Research. 2・3. 187-188 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 渡辺敦史: "PCR-SSCP分析を用いたアカマツとクロマツの葉緑体ゲノム型の簡便判定法" 日本林学会誌. 79・3. 155-156 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 白石 進: "DNAが語るマツの来た道-進化と系統分類-" 林木の育種. 185. 31-35 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-03-31   Modified: 2016-04-21  

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