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PRODUCTION OF HIGHER POLYPLOID STRAINS FOR AQUACULTURE OF CYPRINIFORMES

Research Project

Project/Area Number 09556044
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field General fisheries
Research InstitutionHOKKAIDO UNIVERSITY (1999-2000)
Hiroshima University (1997-1998)

Principal Investigator

ARAI Katsutoshi  Hokkaido Univ., Grad.School of Fish.Sci., Prof., 水産科学研究科, 教授 (00137902)

Co-Investigator(Kenkyū-buntansha) UMINO Tetsuya  Hiroshima Univ., Fac.of Appl.Biol.Sci., Assoc.Prof., 生物生産学部, 助教授 (70232890)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsPolyploid / Cypriniforms / Aquaculture race / Loach / Crucian carp / Gynogenesis / Breeding / Chromosome manipulation / コイ目(もく) / 非還元卵
Research Abstract

(1)Induction and performance of hexaploid loaches : Hexaploid (6n) loaches were produced by inhibiting the second polar body release after fertilization of diploid (2n) eggs with 2n sperm of a natural tetraploid (4n) line. Hexaploid loaches generated 3n gametes. Egg sizes of 6n were larger than those of 4n and 2n loaches. Hexaploid, pentaploid (5n), and tetraploid lines were produced by fertilization of eggs of 6n loaches with sperm of 6n, 4n, and 2n loaches, respectively.
(2)Performance of tetraploid. triploid, and diploid loaches : In communal rearing condition, 2-year-old triploids (3n=2nx4n) showed better growth than 4n and 2n. However, in three-year-old fishes, tetraploids (4n=4nx4n) were smaller than 3n (=4nx2n) and 2n (2nx2n). Gonadal development of 3n was suppressed. Proportion of head length to body length in 4n was larger than those in 2n and 3n.
(3)Performance of hexaploid, pentaploid, and tetraploid loaches : In 1-year-old fish, pentaploids (5n=6nx4n) gave better growth than 6n (6nx6n) and 4n (6nx2n). In communal rearing condition, growth of 3-year-old hexaploid was poor ; about half body weight of 5n and 4n loashes. Hexaploids and pentaploids gave 1 : 1 sex ratio, but tetraploids were all-female. Proportion of head length to body length in 6n was larger than those in 5n and 4n.
(4)Unreduced eggs in loaches : Triploid (2nx4n) loaches gave unreduced 3n eggs. First generation of triploid gynogens induced from such eggs also produced 3n eggs, but a small number of aneuploid (1.5n) eggs were observed. DNA fingerprinting revealed that second generation gynogens induced from these unreduced eggs were aclonal, but genetically very similar. Diploid loach which generated unreduced 2n eggs was also found in the wild population
(5)Production of tetraploid crucian carp : Tetraploidy was induced in gynogenetic triploid crucian carp by incorporating sperm genome into eggs by means of cold, heat and pressure shock of fertilized eggs.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Arai,Katsutoshi: "Production of second generation progeny of hexaploid loach"Fisheries Science. 65(2). 186-192 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Arai,Katsutoshi: "Clonal nature of gynogens induced from spontaneous diploid eggs in the loach Misgurnsu anguillicaudatus"Folia Zoologica. 49(Suppl.1). 31-36 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 間田康史: "雌性発生ギンブナにおける精子核導入による四倍体の作出"水産増殖. 49(1)(印刷中)(2001年3月末発行予定). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsutoshi Arai, Kou Taniura, and Quanqi Zhang.: "Production of second generation progeny of hexaploid loach"Fisheries Science. vol.65. 186-192 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsutoshi Arai, Kagayaki Morishima, Shu-ichi Momotani, Noriko Kudo, and Quanqi Zhang: "Clonal nature of gynogens induced from spontaneous diploid eggs in the loach, Misgurnus anguillicaudatus"Folia Zoologica. vol.49 (Suppl.1). 31-36 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yasufumi Mada, Makiko Miyagawa, Taiga Hayashi, Tetsuya Umino and Katsutoshi Arai: "Production of tetraploids by induction of sperm nucleus into the eggs of gynogenetic triploid ginbuna Carassius langsdorfii"Suisanzoshoku. vol.49 (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Arai, Katsutoshi: "Clonal nature of gynogens induced from spontaneous diploid eggs in the loach Misgurnsu anguillicaudatus"Folia Zoologica. 49(Suppl.1). 31-36 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 間田康史: "雌性発生ギンブナにおける精子核導入による四倍体の作出"水産増殖. 49(1)(印刷中2001年3月未発行予定). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Arai: "Clonal nature of gynogens induced from spontaneous diploid eggs in the loach, Misgurnus anguillicaudatus"Folia Zoologica. (予定). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Arai et al.: "Production of second generation progeny of hexaploid loach" Fisheries Sci.65・2(発売予定). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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