| Project/Area Number |
09556046
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Tokyo University of Fisheries |
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Principal Investigator |
WATANABE Etsuo Tokyo University of Fisheries, Faculty of Fisheries, Professor, 水産学部, 教授 (00017055)
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| Co-Investigator(Kenkyū-buntansha) |
ENDO Hideaki Tokyo University of Fisheries, Faculty of Fisheries, Associate Professor, 水産学部, 助教授 (50242326)
HAYASHI Tetsuhiro Tokyo University of Fisheries, Faculty of Fisheries, Professor, 水産学部, 教授 (00173013)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1998: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1997: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | sodium channel / frogs bladder / sodium electrode / tetrodoxin / swellfish / fish and shellfish toxin / channel blocker / チャネル阻害物質 / カキ / 海藻 / 漢方薬草 / プランクトン |
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| Research Abstract |
The sensor for the determination of NaィイD1+ィエD1 channel blockers consisted of frogs bladder, NaィイD1+ィエD1 electrode, flow cell, peristaltic pump and recorder. The basic conditions for the determination of the Na channel blockers were established as follows.
Preparation of Na channel electrode : The tip of NaィイD1+ィエD1 electrode was covered by the frogs bladder membrane sandwiched between 2 sheets of cellulose acetate membrane. This electrode was integrated within a flow cell connected to a peristaltic pump and injection port.
Some conditions for the determination of a channel blockers : The initial carrier solution contained 8% NaCl and adjusted to pH 4.8 was transferred into the flow cell with 0.8 ml/min at 30℃. Once the background signal had stabilized, 50 ul of TTX sample solution was injected into the sensor system and the response was measured.
Preparation of sample : Toxins of swellfish and shellfish was extracted from each 1 g of them with 0.1% acetic acid and hydrochloric acid at 100℃ for 10 min, respectively and planktons toxin was also extracted from the filter paper which the plankton was corrected on with 0.01 M acetic acid at 100 ℃ for 20 min. NaィイD1+ィエD1 channel blockers contained into seaweed and herb of Chinese medicine was extracted from 1 g of dried seaweed with 0.1 N hydrochrolic acid at 100℃ for 10 min and 20 to 30 g of the dried herb after refluxing with hot water at 90℃ for 1 hr, respectively.
Toxins of swellfish and plankton were determined under the conditions mentioned above. NaィイD1+ィエD1 channel blockers contained in seaweed and herb of chinese medicine were also searched.
From these results, It had become feasible to determine NaィイD1+ィエD1 channel blockers rapidly, simply, high sensitively and continuously.
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