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A study on the clinical application of cytokine therapy in animals

Research Project

Project/Area Number 09556067
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Applied veterinary science
Research InstitutionThe University of Tokyo

Principal Investigator

WATARI Toshihiro (1998)  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant Professor, 大学院・農学生命科学研究科, 助手 (50220950)

長谷川 篤彦 (1997)  東京大学, 大学院・農学生命科学研究科, 教授 (90011923)

Co-Investigator(Kenkyū-buntansha) IWATA Akira  Nippon Institute for Biological Sciences, Project leader, 研究部, プロジェクトリーダー (70193745)
HASEGAWA Atsuhiko  Nihon University, Faculty of Bioresource Sciences, Professor, 生物資源科学部, 教授 (90011923)
TSUJIMOTO Hajime  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Prof, 大学院・農学生命科学研究科, 教授 (60163804)
亘 敏広  東京大学, 大学院・農学生命科学研究科, 助手 (50220950)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 1998: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1997: ¥7,200,000 (Direct Cost: ¥7,200,000)
KeywordsCat / Cytokine / G-CSF / Thrombopoietin / Chemokine / Feline immunodeficiency virus / Chemotherapy / Clinical application / 獣医臨床 / TPO / IL-1ra / 造血幹細胞 / 馬 / 猫
Research Abstract

Leukopenia and thrombocytopenia induced after the treatment with anti-cancer drugs are one of the major problems in small animal practice. To resolve these problems, we carried out molecular cloning of feline granulocyte-colony stimulating factor (G-CSF) and thrombopoietin (TPO) and investigated their clinical application to small animal practice. Feline G-CSF cDNA was amplified by PCR from CRFK cell line cDNA and cloned into a plasmid vector. The cDNA inserted into a baculovirus vector was expressed in insect cells. The recombinant cat G-CSF was shown to be biogically active in a G-CSF-responsive cell line, NFS-60. Feline TPO was amplified by PCR from liver cDNA of a cat with marked thrombocytopenia. The feline TPO cDNA showed high amino acid sequence similarities with caninie and human TPO cDNAs, and contained amino acid motifs conserved among TPOs of different species. The feline TPO cDNA inserted into a mammalian cell expression vector was transfected into 293T cells. The supernatant of the transfectant was shown to have a biological activity in UT-7/TPO cells in a dose-dependent manner. Biotechnology system to produce a large amount of recombinant feline G-CSF and TPO was prepared, and the effects of these cytokines in cats are now investigated.
On the other hand, feline cDNA clones of CC-chemokines (MIP-1alpha, MIP-1beta, RANTES) and CXC-chemokines (SDF-1beta), which have been identified as ligands of co-receptors for human immunodeficiency virus, were isolated and characterized. Of these chemokines, SDF-1 was found to inhibit the growth of feline immunodeficiency virus in cat T-cells in a dose-dependent manner, therefore, we are now studying its application to clinical use for the control of FIV infection.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Matsushiro,H.et al.: "Molecular cloning and functional expression of feline thrombopoietin." Vet.Immunol.Immunopathol.66. 225-236 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Endo,Y.et al.: "Molecular cloning of CC-chemokine cDNAs." Vet.Immunol.Immunopathol.65. 113-123 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mortola,E.et al: "Effect of interleukin-12 and interleukin-10 on the virus replication and apoptosis in T-cells infected with feline immunodeficiency virus." J.Vet.Med.Sci.60. 1181-1185 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mortola,E.et al.: "Inhibition of apoptosis and virus replication in feline immunodeficiency virus-infected cells by N-acetylcysteine and ascorbic acid." J.Vet.Med.Sci.60. 1187-1193 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mizuno.T.et al: "Molecular cloning of feline Fas antigen and Fas ligand cDNAs." Vet.Immunol.Immunopathol.65. 161-172 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Cho,K.W.et al.: "Cloning and mapping of cat (Felis catus) immunoglobulin and T-cell receptor genes." Immunogenetics. 47. 226-233 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsushiro, H.et al.: "Molecular cloning and functional expression of feline thrombopoietin." Vet.Immunol.Immunopathol.66. 225-236 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Endo, Y.et al.: "Molecular cloning CC-chemokine cDNAs." Vet.Immunol.Immunopatho. 65. 113-123 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mortola, E.et al.: "Effect of interleukin-12 and interleukin-10 on the virus replication and apoptosis in T-cells infected with feline immunodeficiency virus." J.Vet.Med.Sci.60. 1181-1185 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mortola, E.et al.: "Inhibition of apoptosis and virus replication in feline immunodeficiency virus-infected cells by N-acetylcysteine and ascorbic acid." J.Vet.Med.Sci.60. 1187-1193 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mizuno, T.et al.: "Molecular cloning of feline Fas antigen and Fas ligand cDNAs." Vet.Immunol.Immunopatho.65. 161-172 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Cho, K.W.et al.: "Cloning and mapping of cat (Felis catus) immunoglobulin and T-cell receptor genes." Immunogenetics.47. 226-233 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsushiro,H.et al: "Molecular cloning and functional expression of feline thrombopoietin." Vet.Immunol.Immunophathol.66. 225-236 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Endo,Y.et al.: "Molecular cloning of CC-chemokine cDNAs." Vet.Immunol.Immunopathol.65. 113-123 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mortola,E.et al: "Effect of interleukin-12 and interleukin-10 on the virus replication and apoptosis in T-cells infected with feline immunodeficiency virus." J.Vet.Med.Sci.60. 1181-1185 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mortola,E.et al.: "Inhibition of apoptosis and virus replication in feline immunodeficiency virus-infected cells by N-acetylcysteine and ascorbic acid." J.Vet.Med.Sci.60. 1187-1193 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mizuno,T.et al: "Molecular cloning of feline Fas antigen and Fas ligand cDNAs." Vet.Immunol.Immunopathol.65. 161-172 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Cho,K.W.et al: "Cloning and mapping of cat(Felis catus)immunoglobulin and T-cell receptor genes." Immunogenetics. 47. 226-233 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mohamed,A., et al.: "Establishment of a sandwich enzyme linked immunosorbent assay for canine interleukin-8." Journal of Veterinary Medical Science. 59. 39-41 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Youn,H.-Y., et al.: "Genomic structure of the bovine mb.-1 gene encoding Ig-alpha subunit of the B cell antigen receptor complex." Veterinary Immunology and Immunopathology. 56. 247-257 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kato,H., et al.: "Molecular cloning and functional expression of equine interleukin-1 receptor antagonist." Veterinary Immunology and Immunopathology. 56. 221-231 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mizuno,T., et al.: "Apoptosis enhanced by soluble factor produced in feline immunodeficiency virus infection." Journal of Veterinary Medical Science. 59. 1049-1051 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mizuno,T., et al.: "Molecular cloning of feline Fas antigen and Fas ligand and their expression in feline immunodeficiency virus infection." Veterinary Immunology and Immunopathology. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Endo,Y., et al.: "Molecular cloning of feline CC-chemokine cDNAs." Veterinary Immunology and Immunopathology. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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