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Clinical application of a biodefense reaction against oxidative stress using mAb.

Research Project

Project/Area Number 09557015
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Pathological medical chemistry
Research InstitutionTokyo medical and dental university, Medical Research Institute

Principal Investigator

YAMAGUCHI Tokio  Tokyo Med.& Dent.Univ., Medical. Res.Inst., Associate Prof., 難治疾患研究所, 助教授 (30134745)

Co-Investigator(Kenkyū-buntansha) SUGIMOTO Akiko  Tokyo Med.& Dent.Univ., Inst.Med.& Dent.Engineering, Res.Assistant, 医用器材研究所, 助手 (60143608)
HORIO Fumihiko  Nagoya Univ., Dept.of Agriculture, Associate Prof., 農学部, 助教授 (20165591)
SUEMATSU Makoto  Keio Univ.Dept.of Medicine, Associate Prof., 医学部, 助教授 (00206385)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsbilirubin / antioxidant / heme oxygenase / endotoxin / ascorbic acid / ELISA / ビリベルジン
Research Abstract

We examined the possibility that bilirubin oxidation is provoked in vivo by using scurvy-prone ODS-odlod rats treated with endotoxin (lipopolysaccharide). Recently, bilirubin oxidative metabolites were isolated from human urine and named biotripyrrin-a and biotripyrrin-b. In ODS-odlod rats fed an ascorbicacid-free diet, the concentration of bilirubin metabolites in urine was increased 7.0-fold at 3 h after injection of lipopolysaccharide and 4.4-fold at 10 h compared to the control rats injected with saline. The dietary supplement of ascorbic acid, the physiological antioxidant, suppressed the increase in bilirubin metabolites in urine after lipopolysaccharide injection : concentrations of biotripyrrin-a and biotripyrrin-b in urine collected 6.5- 1O h after the injection were lower in rats fed an ascorbic-acid-supplemented diet than in rats fed an ascorbic-acid-free diet. Moreover, feeding of ascorbic acid suppressed the hepatic mRNA level of heme oxygenase-1, the rate-limiting enzyme of bilirubin biosynthesis, in rats injected with lipopolysaccharide. These findings indicate that bilirubin oxidation is markedly stimulated in lipo-polysaccharide-treated rats and suggest that bilirubin and ascorbic acid have physio1ogically protective effects against oxidative stress.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Yuichi Shinoda et al.: "Carbon monoxide as a regulator of bile canalicular in cultured rat hepatocytes." Hepatology. 28(2). 286-296 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tokio Yamaguchi et al.: "Bilirubin oxidation provoked by treatment is suppressed by feed-ing ascorbic acid in a mutant unabled to synthesize ascorbic asid" Eur.J.Biochem.245. 233-240 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsuyoshi Sano et al.: "Endogeneous carbon monoxide suppression stimulates bile acid-dependent biliary transport in perfused rat liver." Am.J.Physiol.272. G1268-G1275 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadashi Sakai et al.: "Severe oxidative stress is thought to be a principal cause of jaundice of yellowtail Seriola Quingeradiata." Aquaculture. 160. 205-214 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tokio Yamaguchi et al.: "Tourocholate induces directional excretion of bilirubin into biles in the perfused rat liver." Am.J.Physiol.270. G1028-G1032 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tokio Yamaguchi et al.: "Role of bilirubin as an anti-oxidant in an ischemia-reper-fusion of rat kuer and induction of heme oxygenase" Biochem.Biophys.Res.Commun.223. 129-135 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山口登喜夫 他: "Free Radicals in Clinical Medicineフリーラジカルの臨床 Vol11" 日本医学館(監修 : 近藤元治), 119ページ 担当79-84pp. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shinoda, Y., Suematatsu, M., Wakabayashi, Y., Goda, N., Suzuki, T., Saito, T., Yamaguchi, T., et al.: "Carbon monoxide as a regulator of bile canalicular in cultured rat hepatocytes." Hepatology. 28 (2). 286-296 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi, T., Hashizume, T., Tanaka, M., Nakayama, M., Sugimoto, A., Ikeda, S., Nakajima, H., and Horio, F.: "Bilirubin oxidation provoked by endotoxin treatment is suppressed by feeding ascorbic acid in a rat mutant unable to synthesize ascorbic acid." Eur.J.Biochem.245. 233-240 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sano, T., Shimomi M., Wakabayashi, Y., Shinoda, Y., Goda, N., Yamaguchi, T., Nimura, Y., Ishimura, Y., and Suematsu, M.: "Endogeneous carbon monoxide supression stimulates bile acid-dependent biliary transport in perfused rat liver." Am.J.Physiol.G1268-G1275 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi, T., M.Terakado, F.Horio, K.Aoki, M.Tanaka, and H.Nakajima: "Role of bilirubin as an antioxidant in an ischemia-reperfusion of rat liver and induction of heme oxygenase." Biochem. Biophys. Res.Commun.223. 129-135 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi, T., Y.Wakabayashi, M.Tanaka, T.Sano, H.Ishikawa, H.Nakajima, M.Suematsu, et al.: "Taurocholate induces directional excretion of bilirubin into biles in the perfused rat liver." Am.J.Physiol.270. G1028-G1032 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yuichi Shinoda et al: "Carbon monoxide as a regulator of bile canalicular in cultured rat hepatocytes." Hepatology. 28(2). 286-296 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tokio Yamaguchi et al.: "Bilirubin oxidation provoked by treatment is suppressed by feed-ing ascorbic acid in a mutant wrabled to synthesize oscorbic acid" Eur.J.Biochem.245. 233-240 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsuyoshi Sano et al.: "Endogeneous carbon monoxide suppression stimutales bile acid-dependent biliary transport in perfused rat liver" Am.J.Physiol.272. G1268-G1275 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tadashi Sakai et. al.: "Severe otidative stress is thouglt to be a principal cause of jaundice of yellow tail Seriola Quingeradiota." Aquaculture. 160. 205-214 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tokio Yamaguchi,et al.: "Taurocholate induces directional excretion of bilivubin into biles in the perfused rat hver." An.J.Physiol.270. G1028-G1032 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tokio Yamaguchi et al: "Role of bilirubin as an onti-oxidant in an isehemia-reperfusion of rat liver and induction of heme oxigenase" Biochem.Biophys.Ras.Commun.223. 129-135 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山口登喜夫 他: "Free Radicals in Clinical Medicineフリーラジカルの臨床Vol11" 日本医学館(監修:近藤元治), 119 担当79-84 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tokio Yamaguchi et al.: "Bilirubin oxidation provoked by trentment is suppressed by feeding ascorbic acid in a mutant urable to synthesize oscorbic accd." Eur.J.Biochem.245. 233-240 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsuyoshi Sano et al.: "Endogeneous carbon monoxide sppression stimulates bile acid-deperdent biliary transport in perfused rat liver." Am.J.Physiol.272. G1268-G1275 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yuichi Shimoda et al.: "Carbon monoxide as a regulator of bile caraliclar in cltured rat Repatoryets." Hepatology in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tadashi Sakai et al.: "Severe oxidative stress is thouglt to be a prin is pal cause of jaundica of yellowtail Seriola quinqsradiata." Aqaclture in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tokio Yamaguchi et al.: "Tauhocho late in duces directconal excretion of bilirubin into brles in the perfused rat liver." Am.J.Physid.270. G1028-G1032 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tokio Yamaguchi et al.: "Role of bilimbin as an antioxidant in an is chemia-reperfusion of rat liver and induotion of heme oxygenace" Biochem.Biophys.Res.Commun.223. 129-135 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 山口登喜夫 他: "Free Rochicals in dinical Mediane フリーラジカルの臨床Trol 11." 日本医学館(監修:近藤元治), 119担当79-84 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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