| Project/Area Number |
09557021
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Experimental pathology
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| Research Institution | KOBE UNIVERSITY |
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Principal Investigator |
KITAZAWA Riko KOBE UNIVERSITY SCHOOL OF MEDICINE,2ND DEPARTMENT OF PATHOLOGY,ASSISTANT PROFESSOR, 医学部, 講師 (00273780)
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| Co-Investigator(Kenkyū-buntansha) |
KITAZAWA Sohei KOBE UNIVERSITY SCHOOL OF MEDICINE,2ND DEPARTMENT OF PATHOLOGY,ASSISTANT PROFESS, 医学部, 講師 (90186239)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥8,800,000 (Direct Cost: ¥8,800,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | postmenopausal osteoporosis / osteoclast / stromal cell / RANKL / TRANCE / ODF / OPGL / promoter / in situ hybridizatin / cDNAクローニング |
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| Research Abstract |
Osteoclasts are multinucleated cells derived from hemopoietic cells. Osteoblastic/ stromal cell lineage contributes to the terminal differentiation of osteoclast precursor s through the direct cell-to-cell interaction mechanism (Takahashi et al., 1988). ST2 cells, mouse stromal cell line, support osteoclastogenesis in the coculture with hematopoietic cells in the presence of Vitamin D3 and Dexamethasone. We found the phenomenon of the passage-dependent reduction of osteoclastogenesis in the co-culture system of ST2 and hemopoietic cells. In this study we attempted to isolate the molecules of which gene expression is associated to the passage-dependent capability of supporting osteoclastogenesis, and got 1 .5 kb cDNA of that molecule.
Recently osteoclast differentiation factor (RANKL/ TRANCE/ OPGL/ ODF) was identified by others (H.Yasuda et al., P.N.A.S., 1998 ; D.Lacy et al., Cell, 1998). We then cloned and analyzed of 5-flanking basic promoter region of the mouse RANKL gene and attempted to elucidate the regulatory mechanism of RANKL gene expression in relation to the passage-dependent capability of ST2 cells to support osteoclastogenesis. To assess the expression of genes involved in osteoclastogenesis on the bone tissues, we set up the system of in situ hybridization using decalcified bone specimen. We have presented our data at various international as well as domestic meetings, and publish a lot of scientific papers for academic journals.
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