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Genotoxicity of airborne particles from the urban area in Sapporo : Evaluation of the particulate samples collected over 22 year by bioassays

Research Project

Project/Area Number 09557033
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Public health/Health science
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

SATOH Hiroshi  Graduate School of Medicine, Tohoku University, Professor, 大学院・医学系研究科, 教授 (40125571)

Co-Investigator(Kenkyū-buntansha) NOHMI Takehiko  Genetics and Mutagenesis, National Institute of Health Science, Chief investigator, 変異遺伝部, 主任研究員 (30150890)
MATSUMOTO Yutaka  Hokkaido Research Institute for Environmental Science, Chief investigator, 環境保全部, 主任研究員
SHIMIZU Hidesuke  School of Medicine, The Jikei University, Professor, 教授 (80056879)
TANAKA Noriho  Laboratory of Cell Toxicology, Investigator, 秦野研究所・細胞毒性学研究室, 研究員
永瀬 久光  岐阜薬科大学, 薬学部, 教授 (40141395)
佐藤 孝彦  岐阜薬科大学, 薬学部, 教授 (50082970)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 1999: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1998: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1997: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsUrban airborne particulate / Nitroarene / Polynulear Aromatic Hydrocarbon / Genotoxicity / Ame test / Mouse lymphoma mutation assay (MLA) / In vitro micronucleus test / Nitroarene metabolic enzyme deficient strain / Ames test / YG7126 / 7130株 / L5178Y細胞 / ニトロ還元酵素欠損株 / PAH / Ames-test / YG7126-7130株 / TA1535 / pSK1002菌株
Research Abstract

To investigate genotoxicity of airborne particles, the airborne particulate samples collected in Sapporo, the most northern large city in Hokkaido, Japan, during the period of 24 years from 1974 were analyzed. The results obtained are followings :
1. Concentrations of carcinogenic substances and mutagenicity assayed by Ames test.
Concentrations of polycyclic aromatic hydrocarbons (PAHs) markedly decreased during the observation period of 24 years, while concentrations of nitroarenes did not. Mutagenic activities assayed by TA98 and TA100 strains did only slightly decrease. These results indicates that concentrations of PAHs is not an appropriate indicator for the atmospheric risk of lung cancer and further the risk to human health did not significantly decrease over the observation period in terms of mutagenicity in airborne particles.
2. Mutanenicity assayed by mouse lymphoma cells.
Mutagenicity assay using the L5178Y cell, a cell line of mouse lymphoma cells, efficiently detected mutatio … More ns and chromosomal aberration induced by the airborne particulate samples. The indirect mutagenic activities (+S9) clearly decreased over the period, while direct mutagenic activities (-S9) did not.
3. Mutagenicity assayed by in vitro micronucleus test.
It was shown that mutagenicity of airborne particles is possibly assayed by in vitro micronucleus test using Chinese hamster lung fibroblast cells. The incidences of induced micronucleus were rather stable for +S9 test system over the observation period. For the -S9 test system, the incidences were elevated in early 1980s with small fluctuations for other years.
4. Development of new test strains with metabolic deficits.
By gene disruption, new test strains for Ames assay, YG7128 and YG7132 with completely and partially inhibited nitroreductase activities, were established. YG7126 and YG7130, O-acetyltransferase deficient strains, were also established. These strains effectively detect nitroarenes and it was shown that contribution by nitroarenes was considerable among the total mutagenic activities in the airborne particulate samples. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Yamada M.et al.: "Targeted disruption of the gene encoding the classical nitroreductase enzyme in Salmonella typhimurium Ames test strains TA1535 and TA1538"Mutation Reserch. 375. 9-17 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 能美健彦他: "ニトロレーンに高感受性を示す変異原性試験法の開発"第38回大気環境学会. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 清水英佑他: "培養細胞を用いた小核試験による大気汚染物質の検討"日本環境変異原学会第26回大会. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe M.et al.: "Purification and characterization of wild-type and mutant"classical"nitroreductases of Salmonella typhimurium"The Journal of Biological Chemistry. 273. 23922-23928 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto Y.et al.: "Long-term trends of particulate mutagenic activity in the atmosphere of Sapporo"Environmental Science and Technology. 32. 2665-2671 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 酒井茂克他: "大気浮遊粉塵中多環芳香族炭化水素及びニトロアレーンの粒経分布"第39回大気環境学会. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 松本寛他: "大気浮遊粉塵抽出物の塩基対置換型突然変異スペクトル"第39回大気環境学会. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 松本寛: "変異原性を指標とする大気汚染の健康影響評価法について"日本分析化学会北海道支部平成10年度公開セミナー. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Espinosa-Aguirre J.J.et al.: "New O-acetyltransferase-deficient Ames Salmonella strains generated by specific gene disruption"Mutation Reaerch. 439. 159-169 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 若栗忍他: "札幌市内で採取した大気粉塵抽出物のマウスリンフォーマ試験による突然変異の検出"食品薬品安全センター秦野研究所年報. 22. 43-48 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 関良子他: "大気汚染物質のin vitro小核試験による検討(抄録)"日本衛生学雑誌. 54. 221 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 関良子他: "大気汚染物質のin vitro小核試験による検討"第69回日本衛生学会. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto Y.et al.: "Mutagenic activity airborne particulates : A survey over nearly a quarter century(1974-1998)in Sapporo"First International Symposium on Atmospheric Reactive Substances. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 松本寛他: "大気中変異原活性の長期変動"第26回環境保全・公害防止研究発表会. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 関良子他: "培養細胞を用いた小核試験による大気汚染物質の検討(その2)"日本環境変異原学会第28回大会. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 松本寛他: "大気浮遊粉塵変異原性の地点別・季節別プロファイル"日本環境変異原学会第28回大会. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto Y.et al.: "Mutagenic activity airborne particulates : A survey over nearly a quarter century(1974-1998)in Sapporo"A Monograph on Atmospheric Reactive Substances. (in Press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 酒井茂克他: "大気浮遊粉塵変異原性の地点別・季節別プロファイル"第9回環境化学討論会. (発表予定). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 松本寛他: "変異原性から見た過去四半世紀における札幌市大気粉塵の質的変遷"第9回環境化学討論会. (発表予定). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamada, M., J. J. Espinosa-Aguirre, M. Watanabe, K. Matusi, T. Sofuni and T. Nohmi: "Targeted disruption of the gene encoding the classical nitroreductase enzyme in Salmonella typhimurium Ames test stains TA1535 and TA 1538"Mutation Research. 375. 9-17 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe, M., T. Nishino, K. Takio, T. Sofuni and T. Nohmi: "Purification and characterization of wild-type and mutant "classical" nitroreductases of Salmonella typhimurium. L33R mutation greatly diminishes binding of FMN to the nitroreductase of S. typhimurium"The Journal of Biological Chemistry. 273. 23922-23928 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto, Y., S. Sakai, T. Kato, T. Nakajima and H. Satoh: "Long-term trends of particulate mutagenic activity in the atmosphere of Sapporo. 1. Determination of mutagenic activity by the conventional tester strains TA98 and TA100 during an 18-year period (1974-1992)"Environmental Science and Technology. 32. 2665-2671 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Espinosa-Aguirre, J. J., M. Yamada, K. Matsui, M. Wabanabe, T. Sofuni and T. Nohmi: "New O-acetyltransferase-deficient Ames Salmonella strains generated by specific gene disruption"Mutation Research. 439. 159-169 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto, Y., S. Sakai, T. Akutagawa, T. Kato and H. Satoh: "Mutagenic activity of airborne particulates : A survey over nearly a quarter century (1974-1998) in Sapporo"A Monograph on Atmospheric Reactive Substances. (in Press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumoto Y.et al.: "Mutagenic Activity of Airborne Particulates: A survey over nealy a Quarter Century(1974-1998) in Sapporo"A Monograph on Atmospheric Reactive Substances. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 若栗 忍 他: "札幌市内で採取した大気粉塵抽出物のマウスリンフォーマ試験による突然変異の検出"食品薬品安全センター 秦野研究所年報. 22. 43-48 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 関 良子 他: "培養細胞を用いた小核試験による大気汚染物質の検討"日本環境変異原学会 第28回大会(要旨). 106 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] J.J.Espinosa-Aguirre et al.: "New o-acetyltransferase-dificient Ames Salmonella strains generated by specific gene disroption." Mutation Research. in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Matsumoto et al.: "Long-Term trends of particulate mutagenic activity in the atmosphere of sappro;part 1" Environmental Science and Technology. 32(18). 2665-2671 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 清水英佑 他: "大気汚染物質のin vitro 小核試験による検討" 第69回日本衛生学会総会. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamada, M.et al.: "Targeted disruption of the gene encoding the classical nitroreductase enzyme in Salmonella typhimurium Ames test strains TA1535 and TA1538." Mutation Research. 375. 9-17 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 能美健彦他: "ニトロレーンに高感受性を示す変異原性試験法の開発" 第38回大気環境学会(つくば). (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 清水英佑他: "培養細胞を用いた小核試験による大気汚染物質の検討" 日本環境変異原学会 第26回大会(秦野). (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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