| Project/Area Number |
09557038
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Legal medicine
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| Research Institution | Tottori University |
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Principal Investigator |
YUASA Isao Department of Legal Medicine, Faculty of Medicine, Tottori University, Assistant Professor, 医学部, 講師 (00093633)
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| Co-Investigator(Kenkyū-buntansha) |
IKEBUSHI Jun Department of Legal Medicine, Faculty of Medicine, Tottori University, Research Associate, 医学部, 助手 (30150361)
UMETSU Kazuo Department of Forensic Medicine, Yamagata University School of Medicine, Associate Professor, 医学部, 助教授 (10091828)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥11,900,000 (Direct Cost: ¥11,900,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥8,200,000 (Direct Cost: ¥8,200,000)
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| Keywords | Red cell antigen types / Serum protein types / Red cell enzyme types / Nucleotide sequences / DNA polymorphisms / Primers / Paternity tests / 塩基配列 |
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| Research Abstract |
In this projects we have investigated the molecular bases of classical genetic markers in blood to propose simple and rapid genotyping methods. We analyzed (1)ABO, RH and Diego blood group systems as red cell antigens, (2)orosomucoid (alpha-1-acid glycoprotein), group-specific component, inter-alpha-trypsin inhibitor, the R subcomponent of the first component of component, the seventh component of complement, alpha-1-antitrypsin, and the B unit of coagulation factor XIII as serum proteins (3)phosphoglucomutase 1, eaterase D, DNase 1, acetaldehyde dehydrogenase 2, and phosphomannomutase 2 as red cell and other cell enzymes. We also analyzed some null alleles (ORM1ィイD1*ィエD1Q0koln and ITIH1ィイD1*ィエD1Q0iwate), which could cause a false incompatibility between father and child. A nucleotide substitution of de novo mutation, observed in the group-specific component in a German paternity case, was determined. The findings obtained in this study will be useful not only in forensic science but also biological and medical sciences.
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