|Budget Amount *help
¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥9,200,000 (Direct Cost: ¥9,200,000)
1. Sex determination by detection of Y chromosome-specific aiphoid repeat sequence
We have established the conditions for sensitive amplification of the Y chromosome-specific aiphoid repeat sequence by PCR, and succeeded sex determination from a hair shaft of 2 cm in length.
2. Detection of Y chromosomal microsatellite polymorphisms
The allele frequency of a microsatellite DYS 19 locus was estimated to be A=0.064, B=0.068, C=0.423, D=0.226, E=0.211, F=0.004 and C-2=0.004 (N=263) in Japanese living in Okayama and Hiroshima Prefectures. In these alleles, a C-2 allele, migrated between B and C alleles on electrophoretic gels, have not yet been reported in all the races. By the examination of family samples, the usefulness of this STR system in paternity tests was confirmed. Moreover, this STR system was useful for the identification of an assailant/assailants of a sexual assault. The other STR loci on the Y chromosome, DYS 390, DYS391 and DYS395 were also suggested to be useful in paternity tests and in sexual assault cases.
3. Detection of an X chromosome microsatellite HPRT polymorphism
The allele frequency of a microsatellite at HPRT locus on the X chromosome was estimated to be T1=0.105, T24=0.243, T3=0.396, T4=0.200 and T5=0.057 (male=84, female=63) in Japanese living in Okayama Prefecture. This STR locus was suggested to be useful in paternity tests.
4. Detection of the amelogenin gene
We have examined PCR products from various animals including human male and female amplified using primers for a human amelogenin sequence by restriction enzyme analysis and sequencing, and it was suggested that discrimination between those animals could be possible except that the animals were close phylogenetically. Moreover, using fluorescent dye-labeled primers, human male and female could be determined with high sensitivity by PCR.