| Project/Area Number |
09557051
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Gastroenterology
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| Research Institution | University of Tokyo |
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Principal Investigator |
OGATA Itsuro Univ.of Tokyo, Fac.of Med., Assistant Professor, 医学部・附属病院, 助手 (80169169)
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| Co-Investigator(Kenkyū-buntansha) |
KINOSHITA Moritoshi Ohtsuka Pharmaceutical Co., Assay Research Institute, Chief Researcher, 大塚アッセイ研究所・技術部遺伝診断研究室, 室長(研究職)
TOMIYA Tomoaki Univ.of Tokyo, Fac.of Med., Assistant Professor, 医学部・附属病院, 助手 (90227637)
IKEDA Hitoshi Univ.of Tokyo, Fac.of Med., Assistant Professor, 医学部・附属病院, 助手 (80202422)
大野 明彦 東京大学, 医学部・附属病院, 助手 (30223902)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥10,500,000 (Direct Cost: ¥10,500,000)
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| Keywords | TGFα / pro TGFα / mature form of TGFα / immature form of TGFα / hepatocellular carcinoma / differentiation / diagnostic method |
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| Research Abstract |
ProTGFα is processed to become a mature form of TGFα. In this study, we synthesized several kinds of peptides, amino acid sequences of which exist only in the sequence of proTGFα but not in that of the mature form of TGFα. New Zealand white rabbits were immunized with one of the peptides. We developed a sandwich ELISA using immunoglobulin G fraction of the antiserum and polyclonal antibodies against TGFα. Urine collected from normal adults or patients with hepatocellular carcinoma was added with ammonium sulfate at the final concentration of 70 percent and centrifuged. Precipitate fraction was used as an assay sample. The newly developed ELISA did not detect the mature form of TGFα. However, it detected an immature form of TGFα, the molecular weight of which was larger than that of the mature form. The immature form of TGFα was detected in urine from patients with hepatocellular carcinoma but not in that from normal adults. From these results, it is possible that the immature form of TGFα is elevated in patients with hepatocellular carcinoma. Determination of the immature form of TGFα can be useful for diagnosis of hepatocellular carcinoma. We are now analyzing the content of the immature form of TGFα in blood from patients with hepatocellular carcinoma.
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