| Project/Area Number |
09557055
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | KUMAMOTO UNIVERSITY |
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Principal Investigator |
ANDO Masayuki Kumamoto University School of Medicine, 1st Department of Internal Medicine, Professor, 医学部, 教授 (00040204)
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| Co-Investigator(Kenkyū-buntansha) |
MIZOBE Takanori Kumamoto University School of Medicine, Assistance, 医学部, 助手 (30284748)
YAMASAKI Hisato Kumamoto University School of Medicine, Assistance, 医学部・附属病院, 助手 (00271130)
SUGA Moritaka Kumamoto University School of Medicine, Associate Professor, 医学部, 助教授 (20154437)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥8,100,000 (Direct Cost: ¥8,100,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | summer-type hypersensitivity pneumonitis / serological diagnosis / ELISA method / Trichosporon asahii / polysaccharide antigen / monoclonal antibody |
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| Research Abstract |
Summer-type hypersensitivity pneumonitis (SHP) is the most prevalent type of hypersensitivity pneumonitis in Japan. Its main causative antigenic organisms are Trichosporon species (T. asahii and T. mucoides). It is important in diagnosing SHP to prove specific antibody against causative antigen in patient's serum, for the existence of specific antibody indicates the patient had been exposed to, and sensitized to the antigen. Therefore, we attempted to establish a sandwich enzyme-linked immunosorbent assay (ELISA) system which enable evaluation of specific antibody activity in patients' serum samples.
In the present study, we devised an ELISA system using monoclonal antibody specific to T. asahii, to measure antibodies against T. asahii. We evaluated the usefulness of the system by using sera from patients with SHP, patients with other pulmonary diseases, or healthy volunteers. We found specificity and sensitivity of the system were 92.8% and 92.3%, respectively. This system was proved to be useful in diagnosing SHP.
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