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New Assay Methods for Monitoring Radiation Effects.

Research Project

Project/Area Number 09557068
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Radiation science
Research InstitutionThe University of Tokyo

Principal Investigator

SUZUKI Norio  The University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (10010050)

Co-Investigator(Kenkyū-buntansha) HIRANO Kazuya  The University of Tokyo, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (80251221)
ITOH Masamitsu  Yokohama Posts and Telecommunications Hospital, Medical & Research Associate, 医師(研究職) (80176362)
SAKAI Kazuo  The University of Tokyo, Graduate School of Medicine, Lecturer, 大学院・医学系研究科, 講師 (40153837)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsIndicator of sensitivity / X ray induced protein p41 / anti-p41 antibody / MOLT-4 / cell death / MUC1 mucin
Research Abstract

A newly appearing p41 protein after X-irradiation of MOLT4 cells was characterized to access the mechanism(s) and feasibility as a marker of radiation effect. Amount of p41 was radiation dose and time dependent. Newly prepared polyclonal antibody against p41 also stained p42 on western blotting analysis of various tumor cell lines. Amino acid analysis of p41 and p42 showed that p41 was a cleavage product of p42. P42 was generated in vitro by protein synthesis and used as a substrate to identify a particular caspase (unpublished data) activated after X-irradiation of MOLT4 cells.
Expression of MUC1 mucin of HT-29 cells after X-irradiation of 1, 3, 6, 10 Gy were analyzed for several days during cell death. Glycosylated MUC1 mucins (500 kDa and 390 kDa) and underglycosylated MUC1 mucins (350 kDa and 240 kDa) were found increased in dose-dependent manner reaching maximum level in 4 days. Transcriptional activity of MUC1 gene was analyzed by CAT assay containing the 5'-flanking sequence of MUC1 gene. Induction of CAT activity in HT-29 cells was found elevated from day 2 to 4 of X-irradiation. These findings with increased mRNA determined by RT-PCR indicate that transcriptional activity of MUC1 gene in HT-29 cells was upregulated by X-irradiation.
Further characterization is required prior to practical use of these markers to monitor radiation effect, although the present results indicate such possibility.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Matsumoto,Y.et al.: "A gelelectrophoretic analysis for improved sensitivity and specificity of DNA-dependent protein kinase activity." Radiat.Res.(in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hosoi,Y.et al.: "A phosphatidylionsitol 3-kinase inhibitor,wortmannin,induces radioresistant DNA synthesis,and sensitizes cells to bleomycin and ionizing radiation." Int.J.Cancer. 78. 642-647 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakai,K.et al.: "Effect of hyperthermia on the process of radiation-induced cell death." J.Radiat.Res.39. 333-333 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 鈴木紀夫: "放射線感受性について" 癌の臨床. (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumoto,Y.et al.: "A possible mechanism for hyperthermic radiosensitization mediated through hyperthermic lability of Ku subunits in DNA-dependent protein kinase." Biochemical and Biophysical Research Communications. 234. 568-572 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakai,K.et al.: "Effects of an inhibitor of protein kinases on the response to heat treatment in cultured mammalian cells." Int.J.Hyperthermia. 13. 535-545 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakai, K., Suzuki, N., Itoh, H., and Kubodera, A.: "Effects of an inhibitor of protein kinases on the response to heat treatment in cultured mammalian cells." Int.J.Hyperthermia.13. 535-545 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumoto, Y., Suzuki, N., Sasaki, K., Morimatsu, A., Hirano, K., Murofushi, H.: "A possible mechanism for hyperthermic radiosensitization mediated through hyperthermic lability of Ku subunits in DNA-dependent protein kinase." Biochemical and Biophysical Research Communications. 234. 568-572 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hosoi, Y., Miyachi, H., Matsumoto, Y., Ikehata, H., Komura, J., Ishii, K., H.J.Zhao, Yoshida, M., TakaiY., Yamada, S., Suzuki, N., and Ono, T.: "A phosphatidylionsitol 3-kinase inhibitor, wortmannin, induces radioresistant DNA synthesis, and sensitizes cells to bleomycin and ionizing radiation." Int.J.Cancer. 78. 642-647 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakai, K., Suzuki, N.: "Effect of hyperthermia on the process of radiation-induced cell death." J.Radiat.Res.39. 333 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumoto, Y., Umeda, N., Suzuki, N., Sakai, K., and Hirano, K.: "A gel-electrophoretic analysis for improved sensitivity and specificity of DNA-dependent protein kinase activity." Radiat.Res.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumoto,Y.et al.: "A gelelectrophoretic analysis for improved sensitivity and specificity of DNA-dependent protein kinase activity." Radiat.Res.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hosoi,Y.et al.: "A phosphatidylionsitol 3-kinase inhibitor,wortmannin,induces radioresistant DNA synthesis,and sensitizes cells to bleomycin and ionizing radiation." Int.J.Cancer. 78. 642-647 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sakai,K.et al.: "Effect of hyperthermia on the process of radiation-induced cell death." J.Radiat.Res.39. 333-333 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 鈴木紀夫: "放射線感受性について" 癌の臨床. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto,Y.et al.: "A possible mechanism for hyperthermic radiosensitization mediated through hyperthermic lability of Ku subunits in DNA-dependent protein kinase." Biochemical and Biophysical Research Communications. 234. 568-572 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sakai,K.et al.: "Effects of an inhibitor of protein kinases on the response to heat treatment in cultured mammalian cells." Int.J.Hyperthermia. 13. 535-545 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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