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Development of novel gene therapies in the field of liver surgery

Research Project

Project/Area Number 09557104
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Digestive surgery
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

ARII Shigeki  Kyoto Univ., Faculty of Medicine, A.P., 医学研究科, 助教授 (50151171)

Co-Investigator(Kenkyū-buntansha) KAIDO Toshimi  Kyoto Univ., Faculty of Medicine, Associate, 医学研究科, 助手 (80314194)
IMAMURA Masayuki  Kyoto Univ., Faculty of Medicine, P., 医学研究科, 教授 (00108995)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 1998: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1997: ¥8,500,000 (Direct Cost: ¥8,500,000)
Keywordsliver Surgery / gene therapy / VEGF / p53 / cre-loxp / Flt-1 / IL-12 / アデノウィルスベクター / IL-12 / HSV-tkプロモーター
Research Abstract

There are many diseases and pathophysiological status for the liver surgery. Particulary, the most frequent diseases in this field are primary and secondary liver cancers. Furthermore, novel strategies against the liver failure due to various causes should be also resolved.
In the present study, we attempted to develop new strategies against. The above diseases by gene therapies.
We obtained the following results which included the development of the gene therapies in the fields other than liver surgery.
1) We clarified the efficacy of VEGF gene therapy for prevention of sinusoidal endothelial cell damage during cold preservation.
2) We clarified the efficacy of VEGF gene therapy-for prevention of endotoxin induced liver failure.
3) We developed P53 expression of suicide gene with on/off switch by CreィイD1-ィエD1loxp system.
4) We developed gene therapy using soluble Flt-1 expression vector for peritoneal dissemination of cancer.
5) We developed immunogene therapy by using IL-12 gene transfected dendritic cells

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] A.Mori: "Soluble Flt-1 gene therapy for peritoneal metastases using HVJ-cationic liposomes"Gene Therapy. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Furumoto: "Spleen-derived dendritic cells engineered to enhance interleukin-12 production elicit therapevtic antitumor immune responses"Int J Cancer. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] MJ.Gorrin-Rives: "Macropage metalloelastase gene transfer into melanoma cells suppresses primary tomor growth by halting angiogenesis"Clinical Cancer Res. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 森 章: "HVJ-Cationic liposomeによる肝類洞壁細胞および肝癌細胞に対する遺伝子導入の試み"肝類洞壁細胞研究の進歩. 10. 131-133 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 森 章: "胃癌腹膜播腫モデルに対するsolble Flt-1遺伝子治療"消化器科. 30. 46-51 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 森 章: "可溶性Flt-1による腫瘍血管新生の制御"分子がん治療. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A. Mori: "Soluble Flt-1 gene therapy for peritoneal metastasis using HVJ-cationic liposomes"Gene Therapy. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K. Furumoto: "Spleen-derived dendritic cells engineered to enhance interleukin-12 production elicit therapeutic antitumor immune responses"Int J Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] MJ. Gorrin-Rives: "Macropage metalloelastase gene transfer into melanoma cells suppresses primary tumor growth by halting angiogenesis"Clinical Cancer Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A. Mori: "Gene transduction to hepatic sinusoidal cell and hepatic cancer using HVJ-catimic liposome"Progress in Hepatic Sinusoidal Cell Studies. Vol.10. 131-133 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A. Mori: "Soluble Flt-1 gene therapy for peritoneal metastasis"Gastroenterology. Vol.30. 46-51 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Harada: "Memhane-type matrix metalbopiteinase gene is overexpresed in highly invasive hepatocellular carcinoma" Journal of Hepatology. 28. 231-239 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Nakamura: "Expression of the NalCa exchanger emerges in hepatic stellate cells after octivation in association with liver fibrosis" Proceeding National Academic Science USA. 95. 5389-5394 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Takada: "Morphological altermtion of hepatocytes and sinusoidal endothelial cells in rat fatty liver during cold preservation and the protective effect of Repatxy to gewth tastor" Transplantation. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Masaharu Furutani: "Decreased expression and rare mutation of the CIP/WAF 1 gone in humon hepatocellular carcinoma" Cancer Letter. 111. 191-197 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Toshiya Kawaguchi: "Expression of FAS-estrogen recepton fusion protein induces cell deall in pancreatic cancer cell lines" Cancer Letter. 116. 53-59 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mototaka Niwano: "Amelioration of sinusoidal endothelial cell damage by kupffer cell blokade during cold preservation of rat liver" J.Surg.Res.72. 36-48 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shigeki Uchida: "In esophageal squamous carcinoma vascular endothelial grewth factor is associated with P53 mutation" Brit.J.Ca.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Mototaka Niwano: "The inhibition of tumor growth and microvascular angiogenesis by the potent angiogenesis inhibitor,TNP-470 in rats" Surgery Today. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Toshimi Kaido: "Continecus HGF supphy from HGF-expressing fibroblats transplanted into splcen prevents co14-induced acute liver injury in rats" B.B.R.C. 218. 1-5 (1996)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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