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Formation of soft by transplanting and maturing preadipocytes

Research Project

Project/Area Number 09557122
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Orthopaedic surgery
Research InstitutionNAGOYA UNIVERSITY

Principal Investigator

TORII Shuhei  SCHOOL OF MEDICINE,NAGOYA UNIVERSITY,PRFESSOR, 医学部, 教授 (60115607)

Co-Investigator(Kenkyū-buntansha) KOIDE Mikio  TERUMO CORPORATION,TECHNICAL SECTION,RESEARCH MANAGER, 開発3課, 専門研究員
KITAGAWA Yasuo  BIO SCIENCE CENTER,PRFESSOR, 生物分子応答研究センター, 教授 (50101168)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 1998: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1997: ¥8,500,000 (Direct Cost: ¥8,500,000)
Keywordsmesenchymal stem cell / reconstitute basement membrane / adipose tissue / fibroblast growth factor / soft tissue / 間葉系細胞膜
Research Abstract

Connective tissue has the potential ability to renew and remodel itself in response to injury. In this research, we showed for the first time that this characteristic of connective tissue could be used in plastic and reconstructive surgery for reconstruction of a desired volume of lost soft tissue at a desired position. Our results can be summarized as follows. Kawaguchi et al. Reported (Cytotechnology, 26, 354-361,1998) that subcutaneous injection of cultured 3T3-F442A cells (pre-adipocyte) into nude mice resulted in fat pad formation after 4 weeks. Histological section of the pad showed well-developed adipocytes appearing with blood cells. Toriyama et al. demonstrated (Tissue Culture Engineering, 24, 143-146, 1998) that mesenchymal cells migrated and wrapped the injected plug with the capsule when microbeads coated with cultured pre-adipocytes were injected into mice chests. Differentiation into mature adipose cells was found around the beads with bFGF while no differentiation was found around the beads without bFGF.Kawaguchi et al. reported (De novo adipogenesis in mice at the site of injection of basement membrane and basic fibroblast growth factor. Proc Natl Acad Sci U S A.95, 1062-1066, 1998) the results of injecting reconstituted basement membrane (Matrigel) supplemented with bFGF into nude mice. The neovasculization induced within 1 week was followed by migration of endogenous adipose precursor cells, and a clearly visible fat pad was formed within 2 weeks. Such de novo adipogenesis was induced at any position. Thus our results point to a new technique of augmentation of lost soft tissues in plastic and reconstructive surgery.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Yasuo Kitagawa: "De novo formation of adipose tissue and muscle fibers at the site of injecting Matrigel and growth factors." in Animal Cell Technology-from Vaccines to qenetic medicine. 607-612 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuko Kawaguchi: "Matricrine effect of basement membrane components de novo formation of mesenchymal tissues." in Animal Cell Technology. 9. 261-264 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuko Kawaguchi: "De novo adipogenesis in mice at the site of injection of basement membrance and basic fibroblast growth factor" Proc.Natl.Acad.USA. 95. 1062-1066 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 鳥山和宏: "脂肪組織の工学 Engineering of adipose tissue" 組織培養工学. 24.4. 143-146 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuko Kawaguchi: "Reconstitued basement membrane potentiates in vivo adipogenesis of 3T3-F442A cells." Cytotechnology. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasuo Kitagawa, Nobuko Kawaguchi, Kazuhiro Toriyama, and Shuhei Torii: "De novo formation in mice of adipose tissue and muscle fibers at the site of injecting Matrigel and growth factors." Animal Cell Technology-from Vaccines to genetic medicine, Kluwer Academic Publishers. 607-612 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuko Kawaguchi, Eleli Nicodemou-Lena, Kazuhiro Toriyama, Shuhei Torii, and Yasuo Kitagawa: "Matricrine effect of basement membrane components on de novo formation of mesenchymal tissues." Animal Cell Technology ; Basic & Applied Aspects. 9. 261-264 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuko Kawaguchi, Kazuhiro Toriyama, Eleni Nicodemou-Lena, Kazuhiko Inou, Shuhei Torii, and Yasuo Kitagawa: "De novo adipogenesis in mice at the site of injection of basement membrane and basic fibroblast growth factor." Proc.Natl.Acad.Sci.USA. 95. 1062-1066 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazuhiro Toriyama, Nobuko Kawaguchi, Shuhei Torii, and Yasuo Kitagawa: "Tissue engineering of adipose tissue" J.Tissue Culture Engineering. 24(4). 143-146

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuko Kawaguchi, Kazuhiro Toriyama, Eleni Nicodemou-Lena, Kazuhiko Inou, Shuhei Torii, and Yasuo Kitagawa: "Reconstituted basement membrane potentiates in vivo adipogenesis of 3T3-F442A" Cytotechnology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasuo Kitagawa: "De novo formation of adipose tissue and muscle fibers at the site of injecting Matrigel and growth factors." in Animal Cell Technology-from Vaccines to genetic medicine. 607-612 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nobuko Kawaguchi: "Matricrine effect of basement membrane components de novo formation of mesenchymal tissues." in Animal Cell Technology. 9. 261-264 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nobuko Kawaguchi: "De novo adipogenesis in mice at the site of injection of basement membrance and basic fibroblast growth factor" Proc.Natl.Acad.USA. 95. 1062-1066 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 鳥山和宏: "脂肪組織の工学Engineering of adipose tissue" 組織培養工学. 24.4. 143-146 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nobuko Kawaguchi: "Reconstitued basement membrane potentiates in vivo adipogenesis of 3T3-F442A cells." Cytotechnology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Nobuko Kawaguchi: "De novo adipogenesis in mice at the site of injection of basement membrane and basic fibroblast growth factor" Proc. Natl. Acad. Sci. USA. 95. 1062-1066 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 鳥山和宏: "脂肪組織の工学Engineering of adipose tissue" 組織培養工学. 24(3)(印刷中). 13-16 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nobuko Kawaguchi: "Reconstituted basement menbrane potentiates in vivo adipogenesis of 3T3-F442A cells" Cytotechnology. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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