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The establishment of chromosome engineering for the gene cloning

Research Project

Project/Area Number 09557132
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Obstetrics and gynecology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KATO Hidenori  Medical Institute of Bioregulation, Kyushu Univ., Assistant Professor, 生体防御医学研究所, 講師 (60214392)

Co-Investigator(Kenkyū-buntansha) WAKE Norio  Medical Institute of Bioregulation, Kyushu Univ., Professor, 生体防御医学研究所, 教授 (50158606)
MATSUDA Takeo  Medical Institute of Bioregulation, Kyushu Univ., Research Associate, 生体防御医学研究所, 助手 (10304825)
西田 純一  九州大学, 生体防御医学研究所, 助手 (40264113)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1997: ¥7,800,000 (Direct Cost: ¥7,800,000)
Keywordshuman endometrial cancer / suppressor gene / telomere / chromosome pulverization / 784H11 / chromosome 1 / 1q41-42 / replicative senescence / 1番染色体 / 子宮体癌 / 微少核融合 / 癌抑制遺伝子 / YAC / STSマーカー / STF_s / 微小核融合 / STFs
Research Abstract

To identify the locus of a candidate suppressor gene(s) on chr. 1 was a model case for the establishment of chromosome engineering for the gene cloning in this study. A neo-tagged human chromosome 1 that carried an interstitial q arm deletion (del-1q) or a subchromosomal transferable fragment (30A3 STF) derived from a chr. 1q was transferred into human endometrial cancer cell line, HHUA via microcell fusion. The del-1q and the 30A3 STF had the potential to induce the growth arrest and the morphological changes analogous to senescent cell. These results indicate that the target gene that has senescence-inducing activity for HHUA cells is localized to the chr. 1q region defined by D1S510-D1S237 or D1S103-D1S547. To further define the candidate locus, the utilization of telomere targeting vector or chromosome pulverization to make finer chromosome fragment was also studied. It seemed useful but more improvements is necessary for the actual usages. Alternatively, we screened 61 surgically removed endometrial cancer samples for rearrangements or deletions. Finally, we found a high incidence of LOH in the 1q41-42 region defined by the STS D1S225 and D1S459. After identifying a YAC (748H11 by Research Genetics) involved in this region, we transferred it into HHUA. The YAC clone also induce the cellular senescence. By using this YAC DNA, several cDNA clones were isolated from the normal human endometrial cDNA library. Among them, we found one candidate which primary sequence was altered in many endrometrial cancers.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] 加藤秀則 他: "微小核融合法を用いた癌抑制遺伝子解析"組織培養工学. 24. 540-544 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 和氣徳夫 他: "遺伝子治療の現況と展望"日本婦人科学会誌. 51・8. 715-724 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato H et al: "Suprressed tumorigenecity of human endometrial cancer cells by the restored expression of DCC gene"Br. J. Cancer. 82. 459-466 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kaneta Y et al: "Gestational choriocarcinoma whose responsible pregnancy was a complete hydatidiform mole identified by PCR analysis with new sequence tagged site primers."Jpn J Clin Oncol. 29. 504-508 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhou Y et al: "Involvement of mutations in the DPC4 promotor in endometrial carcinoma development."Molecular Carcinogenesis. 25. 64-72 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wake, N.,: "Genetics of gestational trophoblastic diseases."CME Journal of Gynecologic Oncology. S1-S8 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wake N et al: "New Insights in Gynecology and Obstetrics"Genetics of gynecological cancer: molecular events implicated in trophoblastic neoplasia development.. 38-45 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato H et al: "Suppressed tumorigenecity of human endrometrial cancer cells by the restored expression of DCC gene"Br. J. Cancer. 82. 459-466 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kaneta Y et al: "Gestational choriocaricinoma whose responsible pregnancy was a complete hydatidiform mole identified by POC analysis with new sequence tagged site primers"Jpn. J. Clin. Oncol.. 29. 504-508 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhou Y et al: "Involvement of mutations in the DPC4 promotor in endometrial carcinoma development"Molecular Carcinogenesis. 25. 64-72 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wake N et al: "Genetics of gestational trophoblastic diseases"CME Journal of Gynecologic Oncology. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wake N et al: "Genetics of gynecological cancer : molecular events implicated in trophoblastic neoplasia development. Genetics of Gynecological Cancer"New Insights in Gynecology and Obstetrics. 38-45 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato H et al: "Suprressed tumorigenecity of human endometrial cancer cells by the restored expression of DCC gene."Br.J.Cancer. 82. 459-466 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kaneta Y et al: "Gestational choriocarcinoma whose responsible pregnancy was a complete hydatidiform mole identified by PCR analysis with new sequence tagged site primers."Jpn J Clin Oncol. 29. 504-508 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zhou Y et al: "Involvement of mutations in the DPC4 promotor in endometrial carcinoma develoment."Molecular Carcinogenesis. 25. 64-72 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 加藤秀則 他: "卵巣癌と闘うために -遺伝子研究の現況-"臨床婦人科産科. 53. 782-784 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 松田貴雄 他: "8.絨毛癌の分子生物学 V.子宮体部悪性腫瘍の基礎知識"産科と婦人科. 66. 320-325 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 和氣徳夫 他: "遺伝子治療の現況と展望"日本産婦人科学会誌. 51・8. 715-724 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shimizu,A.et al: "CyclinG Contributes to G2/M arrest of cells in response to DNA Damage" Biochemical and Biophysical Research Communications. 242. 529-533 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wake,N.et al: "Involvemem of IGF2 and H19 imprining in choriocarcinoma drvelopmem" International Journal of Gvnecology and Obsteries. 60・1. S1-S8 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wake,N.et al: "Genetics of gestational irophoblastic diseases." CME Journal of Gynecologic Oncology. in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Zhou,Y.et al: "Involvement of mutations in the DPC4 promoter in endometrial carcinoma development." Molecular Carcinogenesis. in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] 加藤 秀則 他: "微小核融合法を用いた癌抑制遺伝子解析" 組織培養工学. 24・14. 540-544 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 加藤 秀則 他: "ヒト癌と癌遺伝子・癌抑制遺伝子・婦人科癌" 現代医療. 30・7. 2005-2009 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wake,N. et al: "In New insights in Gynecology and Obstetrics" Genetics of gynecological cancer:molecular events implicated in trophoblastic neoplasia development., 7 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Vojta,P,V et al: "Evidence for Two Senescence Loci on Human Chromosomel." Genes,Chrs and Cancer. 16. 55-63 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aoyama H: "Specificity of antibodies against rodent transforming growth factor alphaprotein." J.Histochem.cytochem. 45. 695-701 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mastuda T: "Human chromosome 7 carries a putative tumor suppressor gene(s) involved in choriocarcinoma." Oncogene. 15. 2773-2781 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wake N: "Genetics of gestational trophoblastic diseases." CME Journal of Gynecologic Oncology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Wake N: "Genetics of gynecological cancer-molecular events implicated in trophoblastic neoplasia development." The proceeding of X V FIGO. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Kanuma T,et al: "Alterations of the pl6^<INK4A> gene in human ovarian cancers." Molecular Carcinogenesis. 18. 134・141 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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