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Mechanisms of programd cell death and morphogenesis in cranio-facial development.

Research Project

Project/Area Number 09557141
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Morphological basic dentistry
Research InstitutionTokyo Medical and Dental University (1998)
Japanese Foundation For Cancer Research (1997)

Principal Investigator

ICHIJO Hidenori  Tokyo Medical and Dental University Faculty of Dentistry, Professor, 歯学部, 教授 (00242206)

Co-Investigator(Kenkyū-buntansha) SAITOH Masao  Tokyo Medical and Dental University Faculty of Dentistry, Research Fellow of the, 歯学部・日本学術振興会, 特別研究員
TAKEDA Kohsuke  Cancer Institute, Tokyo, Research Assosiate, 生化学部, 嘱託研究員
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 1998: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1997: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsASKI / Apoptosis / MAP-kinase / Traf / Daxx / ASK1 / 口腔組織 / 発生
Research Abstract

The aim of this study is to understand the mechanisms of programmed cell death and morphogenesis in the process of cranio-facial development by analyzing the signal transduction mechanisms through ASK1 (Apoptosis signal-regulating kinase 1). We obtained several lines of evidence that ASK1 activity is regulated by different adaptor molecules.
1) Identification of thioredoxin (Trx) as an ASK1 inhibitor : Through a genetic screen for ASK 1-binding proteins, Trx, a redox protein implicated in anti-apoptotic function, was identified as an interacting partner of ASK 1. Trx associated with the N-terminal part of ASK1 and thereby inhibited ASK1 activity. The implication of Trx as a negative regulator of ASK1 suggests possible mechanisms for the redox regulation of apoptosis signal transduction pathway.
2) Identification of TRAF2 as an activator of ASK1 in TNF signaling : TNF-induced activation of the JNK requires TRAF2. ASK1 is activated by TNF and stimulates JNK activation. Based on these previous results, we investigated the possible connection between TRAF2 and ASK1. ASK1 interacts with and is-activated by TRAF2 overexpression. In untransfected mammalian cells ASK1 rapidly associates with TRAF2 in a TNF-dependent manner. Thus, ASK 1 turned out to be a mediator of TRAF2-induced JNK activation.
3) Identification of Daxx as an activator of ASK1 in Fas signaling : Similar to TRAF2, Fas-specific adaptor molecule called Daxx was found to interact with and activate ASK1. Daxx-ASK1 axis may provide another signaling pathway to Fas-induced apoptosis.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Funato,N.: "Evidence for apoptosis signal-regulating kinase (ASK) l in the regenerating palatal epithelium upon acute injury." Lab.Invest.78. 477-483 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saitoh,M.: "Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) l." EMBO J.17. 2596-2606 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeda,K.: "Identification of a novel bone morphogenetic protein-responsive gene that may function as a noncoding RNA." J.Biol.Chem.273. 17079-17085 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishitoh,H.: "ASKl is essential for JNK/SAPK activation by TRAF2." Molec.Cell. 2. 389-395 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Berestetskaya,Y.V.: "Regulation of apoptosis by alhpa subunits of G12 and G13 proteins via apoptosis signal-regulating." J.Biol.Chem.273. 27816-27823 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chang,H.Y.: "Activation of apoptosis signal-regulating kinase 1 (ASK1) by the adapter protein daxx." Science. 281. 1860-1863 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Funato, N.: "Evidence for apoptosis signal-regulating kinase (ASK) 1 in the regenerating palatal epithelium upon acute injury." Lab.Invest.78. 477-483 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saitoh, M.: "Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1." EMBO J.17. 2596-2606 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeda, K.: "Identification of a novel bone morphogenetic protein-responsive gene that may function as a noncoding RNA." J.Biol.Chem.273. 17079-17085 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishitoh, H.: "ASK1 is essential for JNK/SAPK activation by TRAF2." Molec.Cell. 2. 389-395 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Berestetskaya, Y.V.: "Regulation of apoptosis by alhpa subunits of G12 and G13 proteins via apoptosis signal-regulating." J.Biol.Chem.273. 27816-27823 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chang, HY.: "Activation of apoptosis signal-regulating kinase 1 (ASK1) by the adapter protein daxx." Science. 281. 1860-1863 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Funato,N.: "Evidence for apoptosis signal-regulating kinase(ASK)1 in the regenerating palatal epithelium upon acute injury." Lab.Invest.78. 477-483 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Saitoh,M.: "Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1." EMBO J.17. 2596-2606 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeda,K.: "Identification of a novel bone morphogenetic protein-responsive gene that may function as a noncoding RNA." J.Biol.Chem.273. 17079-17085 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishitoh,H.: "ASK1 is essential for JNK/SAPK activation by TRAF2." Molec.Cell. 2. 389-395 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Berestetskaya,Y.V.: "Regulatio of apoptosis by alhpa subunits of G12 and G13 proteins via apoptosis signal-regulating." J.Biol.Chem.273. 27816-27823 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Chang,H.Y.: "Activation of apoptosis signal-regulating kinase 1(ASK1)by the adapter protein daxx." Science. 281. 1860-1863 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ichijo,H.: "Induction of apoptosis by ASK1,a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." Science. 275. 90-94 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamashita,H.: "Growth/differentiation factor-5induces angiogenesis in vivo." Exp.Cell Res.235. 218-226 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tobiume,K.: "Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase(ASK)1." Biochem.Biophys.Res.Commun.239. 905-910 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Funato,N.: "Evidence for apoptosis signal-regulating kinase(ASK)1 in the regenerating palatal epithelium upon acute injury." Lab.Invest.in press.

    • Related Report
      1997 Annual Research Report
  • [Publications] Wang,T.-H.: "Microtuble-interfering agents activate c-Jun N-terminal kinase/stress-activated kinase (JNK/SAPK) througu both ras and apoptosis signal-regulating kinase(ASK1)pathways." J.Biol.Chem.in press.

    • Related Report
      1997 Annual Research Report
  • [Publications] Saitoh,M.: "Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase(ASK)1." EMBO J.in press.

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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