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Establishment of mouse strains with different allelic combinations of genes controlling taste receptor and transduction mechanisms.

Research Project

Project/Area Number 09557147
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field Functional basic dentistry
Research InstitutionKYUSHU UNIVERSITY (2000)
Asahi University (1997-1999)

Principal Investigator

NINOMIYA Yuzo  Kyushu Univ., Faculty of Dental Science, Prof., 大学院・歯学研究院, 教授 (50076048)

Co-Investigator(Kenkyū-buntansha) SASAMOTO Kazushige  Kyushu Univ., Faculty of Dental Science, Ass.Prof., 大学院・歯学研究院, 助教授 (30128029)
IKENAGA Hiroshi  Kirin Brewery Co. Ltd., Manager, 基盤技術研究所, 主席研究員
IMOTO Toshiaki  Tottori Univ., Faculty of Medical Science, Ass.Prof., 医学部, 助教授 (10109639)
荒木 元英  朝日大学, 歯学部, 助手 (80232049)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1997: ¥3,700,000 (Direct Cost: ¥3,700,000)
KeywordsSweet taste / taste genes / taste variants / receptor / transduction / leptin, gurmarin / 遺伝的変異マウス / 甘味応答 / Sac遺伝子 / db遺伝子 / レプチン / 糖尿病 / 味蕾
Research Abstract

In order to study taste receptor and transduction mechanisms, we bred mouse strains with various allelic combinations of mouse genes in relation to taste sensitivities. We focused on four known genes, dpa, Sac, db and Gpr70. Among them dpa and Sac genes are reported to influence behavioral preferences for sucrose, saccharin and other sweet substances. The dpa gene is thought to be a candidate gene for controlling gurarin-sensitive sweet receptors (GSSWs). The db gene is reported to control leptin receptor through which sweet sensitivity is mondified. Gpr70 is a gene for a putative sweet receptor. We found that N16 generations in the dpa congenic line were subdivided into 3 groups, nonsweet, semisweet, and sweet tasters. This indicates that there is one other gene which participate in segregation between semisweet and sweet tasters. To examine if Sac and Gpr70 would be the same gene, we made partial congenic strains for Sac and Gpr70 loci by using 129 and C57Bl mice, as parent strains. The results suggested that they are different loci with a distance more than 5.0 cM.The Gpr70 gene did not influence saccharin preferences in mice. Possible contribution of the db gene on sweet sensitivity was examined. We found that leptin inhibited sucrose and saccharin responses without affecting responses to other taste stimuli through leptin receptor expressed in taste tissues in lean control mice. There is a possiblity that leptin receptor may express in taste cells which are sensitive to sweet substances but insensitive to gurmarin. In total, we established 8 mouse lines which shows different sensitivities to gurmarin, leptin and saccharin, sucrose and Dphenylalanine.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Ninomiya,Y. et al: "Responses to umami substances in taste bud cells innervated by the chorda tympani and glossopharyngeal nerves1, 2."J.Nutr.. 130. 950S-953S (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawai,K. et al.: "Leptin as a modulator of sweet taste sensitivities in mice."Proc.Natl.Acad.Sci.USA. 97(20). 11044-11049 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Li,X. et al.: "High-resolution genetic mapping of the saccharin preference locus (Sac) and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal chromosome 4."Mamm.Genome.. 12. 13-16 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ninomiya,Y. et al.: "Leptin and sweet taste."Vitam.Horm.. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasamoto,K. et al.: "Analysis of rhythmical jaw movements produced by taste stimulation in rats."J.Nutr.Sci.and Vitaminol.. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakashima,K. et al.: "Behavioral taste similarities and differences among monosodium L-glutamate and glutamate receptor agonists in C57BL mice."J.Nutr.Sci.and Vitaminol.. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ninomiya, Y.et al.: "Responses to umami substances in taste bud cells innervated by the chorda tympani and glossopharyngeal nerves 1,2."J.Nutr.. 130. 950S-953S (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawai, K.et al.: "Leptin as a modulator of sweet taste sensitivities in mice."Proc.Natl.Acad.Sci.USA. 97(20). 11044-11049 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Li, X.et al.: "High-resolution genetic mapping of the saccharin preference locus (Sac) and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal chromosome 4."Mamm.Genome.. 12. 13-16 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ninomiya, Y.et al.: "Leptin and sweet taste."Vitam.Horm.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasamoto, K.et al.: "Analysis of rhythmical jaw movements produced by taste stimulation in rats."J.Nutr.Sci.and Vitaminol.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakashima, K.et al.: "Behavioral taste similarities and differences among monosodium L-glutamate and glutamate receptor agonists in C57BL mice."J.Nutr. Sci. and Vitaminol.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ninomiya,Y. et al.: "Responses to umami substances in taste bud cells innervated by the chorda tympani and glossopharyngeal nerves1,2."J. Nutr.. 130. 950S-953S (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawai,K. et al.: "Leptin as a modulator of sweet taste sensitivities in mice."Proc.Natl.Acad.Sci.USA. 97(20). 11044-11049 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Li,X. et al.: "High-resolution genetic mapping of the saccharin preference locus (Sac) and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal chromosome 4."Mamm.Genome.. 12. 13-16 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ninomiya,Y. et al.: "Leptin and sweet taste."Vitam.Horm.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sasamoto,K. et al.: "Analysis of rhythmical jaw movements produced by taste stimulation in rats."J.Nutr.Sci.and Vitaminol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakashima,K. et al.: "Behavioral taste similarities and differences among monosodium L-gluamate and glutamate receptor agonists in C57BL mice."J.Nutr.Sci.and Vitaminol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ninomiya,Y.et al.: "Sweet taste responses of mouse chorda tympani neurons:Existence of gurmorin-sensitive and -insensitive receptor components"J.Newrophysiol. 81. 3087-3091 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ming,D.et al.: "Blocking taste receptor activation of gustducin inhibits gustatory responses to bitter compounds"Proc.Natl.Acad.Sei.USA. 97. 9903-9908 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ninomiya,Y.et al.: "Responses of the chorda tympani nerve to nonsugar sweeteners and their inhibition by gurmarin in the diabetic db/db mice." Am.J.Physiol.274. R1324-R1330 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ninomiya,Y.: "Reinnervation of cross-regenerated gustatory nerve fibers into amiloride-sensitive and amiloride-insensitive taste receptor cells." Proc.Natl.Acad.Sci.USA. 95. 5347-5350 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ninomiya,Y.et al.: "Reduction of the suppressive effects of gurmarin on sweet taste responses by addition of β-cyclodextrin." Chem.Senses. 23. 303-307 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ninomiya,Y.et al.: "Nacl-prefering NZB/BINJ mice and Nacl-avoiding CBA/J mice have similar amiloride inhibition of chorda tympani responses to Nacl." Chem.Senses. 23. 411-416 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ninomiya,Y.et al.: "Responses to umami substances in taste buds cells innervated by the chorda tympani and glossopharyngeal nerves." J.Nutr.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ninomiya,Y.et al.: "Sweet taste responses of mouse chorda tympani neurons:Evidence for the existence of gurmarin-sensitive and-insensitive receptor components." J.Neurophysiol.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ninomiya,Y: "Lack of gurmarin sensitivity of sweet taste receptors innervated by the glossopharyngeal nerve in C57BL mice" American Journal of Physiology. 272. R1002-R1006 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakashima,K: "Increose in inositol 1,4,5-triphosphate levels of the fungiform papilla in response to saccharin and bitter Substences in mice" Cellular Physiology and Biochemistry. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ninomiya,Y.: "Ennanced responses of the chorda tympani nerve to non-sugar sweeteners in the diabetic db/db mouse" American Journal of Physiology. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ninomiya,Y.: "Reduction of the suppressive effects of gurmarin on sweet taste responses by addition of β-cyclodextrin" Chemical Senses. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ninomiya,Y.: "Nacl-preferring NZB/BINJ mice and NaCl-avoiding CBA/J mice have similar amiloride inhibition of chorda tympani responses to NaCl" Chemical Senses. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hellekant,G.: "Taste in chimpanzees III:Labeled-line coding in sweet taste" Physiology and Behavior. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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