| Project/Area Number |
09557157
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
補綴理工系歯学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
AKAGAWA Yasumasa Hiroshima University, School of Dentistry, Professor, 歯学部, 教授 (00127599)
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| Co-Investigator(Kenkyū-buntansha) |
KOMURA Ikuhiro Hiroshima University, Dental Hospital, Research Associate, 歯学部・附属病院, 助手 (70284213)
TSUGA Kazuhiro Hiroshima University, Dental Hospital, Asistant Professor, 歯学部・附属病院, 講師 (60217289)
HOSOKAWA Ryuji Hiroshima University, School of Dentistry, Research Associate, 歯学部, 助手 (60211546)
WADAMOTO Masayoshi Hiroshima University, Dental Hospital, Asistant Professor, 歯学部・附属病院, 講師 (70231040)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥11,400,000 (Direct Cost: ¥11,400,000)
Fiscal Year 1998: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1997: ¥8,300,000 (Direct Cost: ¥8,300,000)
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| Keywords | Implant / Hydroxyapatite / Titanium / Osteoblast / Cell adhesion / RGD peptide / Specific adhesion |
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| Research Abstract |
Hydroxyapatite (HA) is a bloactive dental implant material which accelerates bone formation on its surface. The mechanism of this acceleration is not clear. The elucidation of the cell adhesion might be the key to understanding the bioactive mechanism of HA.In this study, we analyzed the adhesion of HOS human osteoblasts onto HA and titanium to find out the particular adhesion to HA.In short-term cultures in fetal bovine serum-precoated materials, a significantly higher number of cells adhered to HA than to titanium. In addition, serum-free conditions with phosphate buffered-saline precoating or bovine serum albumin precoating materials were tested. The results were nearly the same among all precoating conditions, suggesting that the quantity of cell adhesion was not affected by serum components. However, in the morphological observation by SEM, the form of adhesion was found to differ among precoating conditions. The osteoblasts tightly adhered and spread onto both HA and titanium with serum precoating, whereas the cells loosely adhered and did not spread without serum. To evaluate the Arg-Gly-Asp (RGD) sequence-specific adhesion, synthetic RGD peptides were used for a competitive inhibition test. The result showed that RGD peptides remarkably inhibited the tight adhesion and spreading of osteoblasts onto HA, whereas it did not strongly inhibit these onto titanium. These results demonstrate that the regulation of cell adhesion to HA is different from that to titanium. Our study suggests that the RGD-containing serum proteins might have a major role in regulating the specific adhesion of osteoblasts to [IA, and in inducing enhanced cell growth and differentiation.
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