| Project/Area Number |
09557169
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | HIROSHIMA UNIVERSITY |
|---|
Principal Investigator |
OSAKI Teruhiko Hiroshima University Scho.of Dentistry, Assistant Prof., 歯学部, 助手 (60243581)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AKAGAWA Yasumasa Hiroshima University Scho.of Dentistry, Professor, 歯学部, 教授 (00127599)
OKAMOTO Tetsuji Hiroshima University School of Dentistry, Professor, 歯学部, 教授 (00169153)
TORATANI Shigeaki Hiroshima University Dental Hospital, Associate Prof., 歯学部・附属病院, 講師 (90172220)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | Periodontal-Ligament Cells / Epithelial rests of Malassez / Serum-Free Culture / FGF-7 / KGF / FGF receptor / Differentiation / Epithelial-Stromal Interaction |
|---|
| Research Abstract |
In this study, we have developed a serum-free culture system of human periodontal ligament-derived epithelial cells (Epithelial rests of Malassez : ME) derived from Hertwig's epithelial root sheath.
The mitogenic effect of fibroblast growth factor (FGF) in the ME and human periodontal ligament-derived fibroblast cells (PLF) were investigated by serum-free growth assay. In addition, we demonstrated the expression of FGF receptor (FGFR) in the ME and the expression of FGF in the PLF by reverse transcription-polymerase chain reaction (RT-PCR), PCR-Southem hybridization, ribonuclease protection assay (RPA) and immunohistochemical examinaijon.
Both FGF-1 and FGF-7/keratinocyte growth factor (KGF) stimulated cell growth of the ME.On the other hand, both FGF-1 and FGF-2 stimulated cell growth of the PLF.RT-PCR and PCR-Southem analysis revealed that the ME expressed FGFR2-(IIIb) mRNA but not mRNAs for FGFR1 -(IIIc), FGFR2-(IIIc), FGFR3-(IIIb), FGFR3-(IIIc) and FGFR4. An immunohistochemical. analysis of FGF-7/KGF revealed that immunoreactive FGF-7/KGF were detected predominantly in the cytoplasm of the. PLF cells. PCR-Southem and RPA also revealed that the PLF expressed FGF-7/KGF mR.NA.
These results suggest that the FGF-FGFR2-(IIIb) system plays an important role in the growth and differentiation of ME.In addition, endogenously produced FGFs work as paracrine interactive regulators of the growth and differentiation between ME and PLF.
|
