| Project/Area Number |
09557191
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biological pharmacy
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
NAGASAWASHIGEHARU Hokkaido Univ., Grad Sch. Pharm. Sci., Pro., 大学院・薬学研究科, 教授 (70029958)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Haoshi Hokkaido Univ., Grad Sch. Pharm. Sci., Inst., 大学院・薬学研究科, 助手 (80241347)
YAMASHITA Toshiyuki Hokkaido Univ., Grad Sch. Pharm. Sci., Inst., 大学院・薬学研究科, 講師 (90192400)
TAKAHASHI Kazuhiko Hokkaido Univ., Grad Sch. Pharm. Sci., Lec., 大学院・薬学研究科, 助教授 (10113581)
SATO Noriyuki Sapporo Med. Univ., Sch. Med., Pro., 医学部, 教授 (50158937)
SEYA Tsukasa Osaka Med. Cent Cancer and Cardiovas. Diseases, Div. Head., 研究所, 部長 (10301805)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1997: ¥7,300,000 (Direct Cost: ¥7,300,000)
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| Keywords | Innate immunity / natural killer cells (NK) / tumor cells / cytotoxicity / complement / M. fermentans / 免疫 / NK細胞 / MHCクラスI / M161抗原 / オプソニン効果 |
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| Research Abstract |
A. We found a novel negative regulator molecule, Cho-1, which is expressed on normal cells and protected host cells from the cytotoxicity by natural killer (NK) cells. Cho-1 was IFN-γ-inducible, but appeared to be different from MHC class I with respect to molecular composition and immunoreactivity. Although this molecule was expressed on certain tumor cell lines, it was not involved in the cytotoxic mechanism of CTL. Thus, Cho-1 appeared to be the novel regulatory molecule in the NK cytotoxic mechansim of innate
B. We found a M 161Ag, which is expressed on human malignant cells and induces homologous complement activaton. cDNA of M161Ag revealed that it is highly homologous to P48, my coplasma fermentans gene product It was revealed that latently infected M, fermentans allows human cells to produce M161Ag, which is a potent modulator of innate and cellular immune responses via its complement activating and cytokine-producing activity.
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