| Project/Area Number |
09557195
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biological pharmacy
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
IDE Toshinori Hiroshima University Faculty of Medicine, Professor, 医学部, 教授 (60012746)
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| Co-Investigator(Kenkyū-buntansha) |
TAHARA Hidetoshi Hiroshima University Faculty of Medicine, Research Associate, 医学部, 助手 (00271065)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥11,700,000 (Direct Cost: ¥11,700,000)
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| Keywords | telomere / telomerase / cellular lifespan / cellular senescence / cellular immortality / cancer diagnosis / テロメラーゼcDNA / hTRT / テロメラーゼ発現 / 細胞癌化 / 癌組織 |
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| Research Abstract |
New findings of this project are 1) telomerase was partially purified from porcine testis to a few protein bands on acrylamidegel electrophoresis, but, since cDNA (hTERT) cloning of assumed catalytic subunit of telomerase has been reported by other reserchers (Aug. 1 997), we abandoned cloning project through protein, purification, 2) expression of hTERT mRNA was found to be correlated with presence 0f telomerase activity, 3) telomerase-negative human fibroblasts expressed telomerase activity after transfection with hTERT cDNA, 4) these telomerase positive human fibroblasts extended both telomere length and proliferative lifespan, but they did not immortalized, 5) telomerase-negative human fibroblasts transformed with SV40 T-antigen also expressed telomerase activity after. transfection with hTERT cDNA, 6) these telomerase-positive SV4O-transformed human fibroblasts extended both telomere length and proliferative lifespan, and they immortalized.
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