| Project/Area Number |
09557198
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biological pharmacy
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
SEKIMIZU Kazuhisa Faculty of Pharmaceutical Sciences, Kyushu University, Professor, 薬学部, 教授 (90126095)
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| Co-Investigator(Kenkyū-buntansha) |
MIKI Takeyoshi Faculty of Pharmaceutical Sciences, Kyushu University, Associate Professor, 薬学部, 助教授 (40037586)
SASAKI Shigeki Faculty of Pharmaceutical Sciences, Kyushu University, Associate Professor, 薬学部, 助教授 (10170672)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 1998: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1997: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | DnaA protein / Esherichia coli / DNA replication / DNA polymerase III / the dnaN genen / oriC replication / DnaAタンパク / ATP / 抗菌剤 / Walkerモチーフ / 部位特異的変異 |
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| Research Abstract |
Initiation of DNA replication in cells is negatively or positively regulated by various factors. We tried to identify those regulating factors by using biochemical and genetic approaches. In crude extacts of E.coli, we identified an activity (RIDA, regulatory inhibition of DnaA protein) which stimulates hydrolysis of ATP bound to DnaA protein, the initiator of DNA replication in E coli. Purification of this factor demonstrated that the beta subunit of DNA polymerase III holoenzyme is responsible for this activity. In a mutant cells of the dnaN gene, which encodes the beta subunit, the ADP-binding form of DnaA protein, which is inactive for DNA replicatoin, was shown to be accumulated. The result confirms that the beta subunit stimulates hydrolysis of ATP bound to DnaA protein. This is the first report indicating that a subunit of DNA polymerase, which is responsible for catalytic reaction of DNA synthesis, negatively regulates initiation of DNA replication.
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