• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Medical application of microencapsulating hybridoma cells in agarose microbeads "Cytomedicine"

Research Project

Project/Area Number 09557202
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 医薬分子機能学
Research InstitutionOsaka University

Principal Investigator

TSUTSUMI Yasuo  School of Pharmaceutical Sciences Osaka University Research Associate, 薬学研究科, 助手 (50263306)

Co-Investigator(Kenkyū-buntansha) MAYUMI Tadanori  School of Pharmaceutical Sciences Professor, 薬学研究科, 教授 (00098485)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1998: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1997: ¥3,700,000 (Direct Cost: ¥3,700,000)
KeywordsCytomedicine / APA-microcapsule / Immunoisolation / Drug Delivery System
Research Abstract

Microencapsulation of living cells or tissues has been proposed to prevent their immune destruction following transplantation. In this study, we examined whether SK2 hybridoma cells microencapsulated in an alginate-poly(L)lysine-alginate (APA) membrane (APA-SK2 cells) were immunoisolated from the allogeneic host's immune system using a cytotoxicity test. The APA membrane inhibited the activation of the host's cellular immune response, but did not prevent the production of cytotoxic antibodies against entrapped SK2 cells following allogeneic transplantation. However, the APA-SK2 cells remained vital in SK2 cell-immunized mice as well as in intact mice. We considered that complement regulatory factors which were present on cell membrane and had species-specific restriction blocked the complement-mediated cell lysis on allogeneic transplantation, since APA-SK2 cells were destroyed by rabbit anti-SK2 cell antiserum. Our results demonstrated that APA membrane could inhibit cell-cell contact between entrapped cells and the host's lymphocytes, but could not completely protect the entrapped cells from xenogeneic hamoral immunity.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report

Research Products

(22 results)

All Other

All Publications (22 results)

  • [Publications] Mayumi T.et al.: "Medical application of microencapsulating hybridoma cells in agarose microbeads “Cytomedicine"" J.Control.Release. 44. 195-200 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mayumi T.et al.: "Immunological studies of SK2 hybridoma cells microencapsulated with alginatepoly(L)lysine-alginate(APA)membrane following allogeneic transplantation." Biochem.Biophys.Res.Commun.230. 524-527 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mayumi T.et al.: "Cytomedical therapy for IgG1 plasmacytosis in human interleukin-6 transgenic mice using hybridoma cells microencapsulated in alginate-poly(L)lysine-alginate membrane." Biochem.Biophs.Acta.1360. 53-63 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mayumi T.et al.: "Therapeutic effect of cytomedicine on mesangio-proliferative glomerulonephritis in human interleukin-6 transgenic mouse." Biol.Pharm.Bull.20. 255-258 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsutsumi Y.et al.: "Tumor necrosis factor alpha-mediated tumor regression by the in vivo transfer of genes in to the artery that leads to tumors." Cancer Res.58. 5725-5730 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mayumi T.et al.: "Possibility of cytomedical therapy for diabetes melitus using microcapsulated pancreatic β cell line with glucose sensor." Drug Delivery System. 13. 95-100 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okada N., Miyamoto H., Kaneda Y., Yamamoto Y., Katsume A., Saito H., Yorozu K., Ueda O., Tsutsumi Y., Nakagawa S., Oshugi Y., and Mayumi T.: "Medical application of microencapsulating hybridoma cells in agarose microbeads "Cytomedicine" ; Therapeutic effect on IgG1 plasmacytosis and mesangio-proliferative glomerrulonephritis in the interleukin-6 transgenic mouse." J.Control.Release. 44. 195-200 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okada N., Miyamoto H., Yoshioka T., Sakamoto K., Katsume A., Saito H., Nakagawa S., Ohsugi Y., Mayumi T.: "Immunological studies of SK2 hybridoma cells microencapsulated with alginate-poly (L) lysine-alginate (APA) membrane following allogeneic transplantation" Biochem.Biophys.Res.Commun.230. 524-527 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okada N., Miyamoto H., Yoshioka T., Katsume A., Saito H., Yorozu K., Ueda O., Itoh N., Mizuguchi H., Nakagawa S., Oshugi Y., and Mayumi T.: "Cytomedical therapy for IgG1 plasmacytosis in human interleukin-6 transgenic mice using hybridoma cells microencapsulated in alginate-poly (L) lysine-alginate membrane." Biochem.Biophs.Acta.1360. 53-63 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okada N., Miyamoto H., Yoshioka T., Katsume A., Saito H., Yorozu K., Ueda O., Nakagawa S., Oshugi Y., and Mayumi T.: "Therapeutic effect of cytomedicine on mesangio-proliferative glomerulonephritis in human interleukin-6 transgenic mouse." Biol.Pharm.Bull.20. 255-258 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mizuguchi H., Nakagawa T., Toyosawa S., Nakanishi M., Imazu S., Nakanishi T., Tsutsumi Y., Nakagawa S., Hayakawa T., Ijuhin N., and Mayumi T.: "Tumor necrosis factor alpha-mediated tumor regression by the in vivo transfer of genes in to the artery that leads to tumors." Cancer Res.58. 5725-5730 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshioka T., Okada N., Miyamoto H., Sakamoto K., Tsutsumi Y., Nakagawa S., Miyazaki J., Mayumi T.: "Possibility of cytomedical therapy for diabetes melitus using microcapsulated pancreatic beta cell line with glucose sensor." Drug Delivery System.13. 95-100 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsutsumi Y.et al: "Tumor necrosis factor alpha-mediated tumor regression by the in vivo transfer of genes in to the artery that leads to tumors." Cancer Res.58. 5725-5730 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsutsumi Y.et al: "Immunological studies of SK2 hybridoma cells microencapsulated with alginate-poly(L)lysine-alginate(APA)membrane following allogeneic transplantation." Biochem.Biophys.Res.Commun.230. 524-527 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mayumi T.et al.: "Possibility of cytomedical therapy for diabetes melitus using microcapsulated pancreatic β cell line with glucose sensor." Drug Delivery System. 13. 95-100 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mayumi T.et al.: "The optimization of cytoplasmic gene expression system with T7 RNA polymerase." Drug Delivery System. 13. 87-93 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mayumi T.et al.: "Development of VSV-liposomes as a novel gene transfer vector" Drug Delivery System. 13. 159-164 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mayumi T.et al.: "Medical application of microencapsulating hybridoma cells in agarose microbeads ″Cytomedicine″" J.Control.Release. 44. 195-200 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mayumi T.et al.: "lmmunological studies of SK2 hybridoma cells microencapsulated with alginate-poly(L)lysine-alginate(APA)membrane following allogeneic transplantation." Biochem.Biophys.Res.Commun.230. 524-527 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mayumi T.et al.: "Cytomedical therapy for lgG1 plasmacytosis in human interleukin-6 transgenic mice using hybridoma cells microencapsulated in alginate-poly(L)lysine-alginate membrane." Biochem.Biophs.Acta.1360. 53-63 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mayumi T.et al.: "Therapeutic effect of cytomedicine on mesangio-proliferative glomerulonephritis in human interleukin-6 transgenic mouse." Biol.Pharm.Bull.20. 255-258 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mayumi T.et al.: "Development of novel drug delivery system of bioactive molecules from ″Cytomedicine″ using hybridoma cells entrapped in APA microcapsules." Drug Delivery System. 12. 107-114 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi