| Project/Area Number |
09557216
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Laboratory medicine
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| Research Institution | Niigata University |
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Principal Investigator |
OKADA Masahiko Niigata University, School of Medicine, Professor, 医学部, 教授 (30018915)
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| Co-Investigator(Kenkyū-buntansha) |
INANO Koichi Niigata University Hospital, Assistant, 医学部・附属病院, 助手 (10262445)
MIIDA Takashi Niigata University Hospital, Lecturer, 医学部・附属病院, 講師 (80260545)
TAKAYUKI Matsuto Niigata University, School of Medicine, Asso Professor, 医学部, 助教授 (80209577)
ITO Yasuki Denka Seiken Co., Ltd. Researcher, 臨床試薬部, 研究員
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1997: ¥5,900,000 (Direct Cost: ¥5,900,000)
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| Keywords | atherosclerosis / low density lipoprotein / oxidized LDL / epitope / antibodies against oxidized LDL / fragment / sugar chain / hyperlipidemia |
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| Research Abstract |
Although there is increasing evidence of anti-oxidized LDL autoantibodies in human sera, the data remain contradictory. Thus, we examined the difference in autoantibody titers between patients with Achilles tendon xanthoma and control subjects using oxidized LDL that was purified by gel-permeation chromatography. Fifteen hyperlipidemic patients with Achilles tendon xanthoma (group A+) and 94 hyperlipidemic patients without Achilles tendon xanthoma (group A-) were studied. Quantification of anti-oxidized and anti-native LDL autoantibodies was performed using an ELISA method. To calculate antibody titers, we used the ratio between the spectrophotometric reading of anti-oxidized and anti-native LDL wells. Using this technique, IgG level differed significantly between groups A+ and A- (p < .01). In contrast using native and oxidized LDL as antigens without FPLC purification revealed no significant difference between the two groups. Furthermore, IgG autoantibody titer did not correlate significantly with age, body mass index, total cholesterol, HDL cholesterol, LDL cholesterol, or triglyceride. Based on these results, IgG autoantibody values appear to correlate with Achilles tendon xanthoma.
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