• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

MEDICAL APPLICATION AND DEVELOPMENT OF EPITOPE DETECTION SYSTEM TO ANTIGEN RELATED AUTO IMMUNE AND ALLERGY DISORDERS

Research Project

Project/Area Number 09557218
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field Laboratory medicine
Research InstitutionNAGOYA CITY UNIVERSITY

Principal Investigator

KUNIMATSU Mitoshi  NAGOYA CITY UNIVERSITY, MEDICAL SCHOOL, LECTURER, 医学部, 講師 (70145746)

Co-Investigator(Kenkyū-buntansha) OHTSUKA Takanobu  NAGOYA CITY UNIVERSITY, MEDICAL SCHOOL, Assistant Professor, 医学部, 講師 (10185316)
TADA Toyohiro  NAGOYA CITY UNIVERSITY, NURSING SCHOOL, PROFESSOR, 看護学部, 教授 (20106230)
ITO Hirotaka  NAGOYA CITY UNIVERSITY, MEDICAL SCHOOL, ASSOSIATE PROFESSOR, 医学部, 助教授 (60080115)
OZAKI Yasuhiko  NAGOYA CITY UNIVERSITY, MEDICAL SCHOOL, RESEACH ASSOSIATE, 医学部, 助手 (50254280)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥11,800,000 (Direct Cost: ¥11,800,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥6,500,000 (Direct Cost: ¥6,500,000)
Keywordsepitope / auto antigen / auto antibody / peptide chip / macro array / immuno-ome / ペプチド / ELISA / collagen / 強皮症 / 慢性関節リウマチ / オーバーラップペプチド / アンピセンス / 水疱性類天疱瘡 / アレルゲン
Research Abstract

A concept of this research is to develop the peptide chips including amino acid sequences of a target proteins to be related to a disease. We attempted medical application and development of epitope detection system to antigen related auto immune and allergy disorders by using this eptide chips. B cell epitopes to calpain, calpastatin and fodrin were analyzed with peptide chips on ce llulose membranes spotted a small amount of peptides. The antibody to each epitope obviously stained the neuronal ischemia regions and myocardial infraction regions. Epitopes to autoantigen CENP-A and Epiplakin were analyzed with its autoimmune disease patient serum. Specific amino acid sequences on these auto antigens were found by using autoimmune disease patient sera. This assay system was useful for screening and diagnosis of autoimmune disease. We succeeded in a producing the peptide chips more over 2,000 knds of peptides on membrane approximately five times, compared with conventional methods. Macro array machine could detect super very small amount of antigens with a fluorescent antibody as probe. This system applied to an antigen dot assay methods. The protein antigen spotted on nitrocellulose membrane, the antigen chip manufactured. Each spot was a very small amount of antigen, 60-to 160 nL/spot. We tried to detect an anti TSH auto antibody in autoimmune disease patient serum. A strong immune reaction obtained from TSH alpha subunit but not its beta subunit. This system was available for kinds of 160,000-tetra peptide library including D-amino acids and amino acid derivatives.
It was able to apply a development of various ferment prevention agent. Now we attempt to make high-density peptide chips synthesis system at high speed further.

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Fu.J.: "MSP1-reactive T cells in individuals in malaria endemic Solomon area and in non-immune Japanese"Parasitology International. 46. 7-16 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fukuda,S.,: "Postischemic reperfusion induces alpha-fodrin proteolysis by mu-calpain in the synaptosome and nucleus in rat brain."J Neurochem. 70. 2526-2532 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hitomi,K.,: "Purification and characterization of the active-site-mutated recombinant human mu-calpain expressed in baculovirus-infected insect cells."Biochem Biophys Res Commun. 246. 681-685 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondo,Y: "Analysis of conserved ambisense sequences within GB virus C"J Infect Dis. 178. 1185-1188 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakuma,N: "a-Tocopherol protects the peroxidative modification of LDL to be recognized by LDL receptors"J Nutr Sci Vitaminol. 44. 697-703 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kunimatsu,M: "Activation of calpain in myocardial infarction : an immunohistochemical study using a calpain antibody raised agaist active site histidine-containing peptide"Cardiovascular Pathology. 8. 7-15 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Saito,T.,: "Protective effects of calpain inhibitor for prolonged hypothermic cardiac preservation"Jpn J Thorac Cardiovasc Surg. 47. 145-152 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Chiang KC: "Clusterin may be involved in rat liver allograft tolerance"Transplant Immunology. 8. 95-99 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nuro,Y: "Autoepitopes on autoantigen CENP-A are restricted to the N-terminal region, which has no homology with histone H3."Clinical & Experimental Immunology. 120. 218-223 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakuma,N.,: "Ascorbic acid protects agaainst peroxidative modification of low-density lipoprotein, maintainiits recognition by LDL receptorsng"J Nutr Sci Vitaminol. 47. 28-31 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujiwara,S.,: "Epiplakin, a novel member of the plakin family originally identified as a 450-kDa human epidermal autoantigen : Structure and tissue localization"J Biol Chem. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fu, J.: "MSP1-reactive T cells in individuals in malaria endemic Solomon area and in non-immune Japanease"Parasitology International. 46. 7-16 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fukuda, S: "Postischemic reperfusion induces alpha-fodrin proteolysis by mu-calpain in the synaptosome and nucleus in rat brain."J Neurochem. 70. 2526-2532 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hitomi, K.: "Purification and characterization of the active-site-mutated recombinant human mu-calpain expressed in baculovirus-infected insect cells."Biochem Biophys Res Commun. 246. 681-685 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondo, Y.: "Analysis of conserved ambisense sequences within GB virus C."J Infect Dis. 178. 1185-1188 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakuma, N.: "a-Tocopherol protects the peroxidative modification of LDL to be recognized by LDL receptors."J Nutr Sci Vitaminol. 44. 697-703 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kunimatsu, M.: "Activation of calpain in myocardial infarction : an immunohistochemical study using a calpain antibody raised agaist active site histidine-containing peptide."Cardiovascular Pathology. 8. 7-15 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Saito, T.: "Protective effects of calpain inhibitor for prolonged hypothermic cardiac preservation."Jpn J Thorac Cardiovasc Surg. 47. 145-152 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Chiang KC: "Clusterin may be involved in rat liver allograft tolerance"Transplant Immunology. 8. 95-99 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Muro, Y.: "Autoepitopes on autoantigen CENP-A are restricted to the N-terminal region, which has no homology with histone H3."Clinical & Experimental Immunol. 120. 218-223 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakuma, N: "Ascorbic acid protects agaainst peroxidative modification of low-density lipoprotein, maintainiits recognition by LDL receptorsng"J Nutr Sci Vitaminol. 47. 28-31 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujiwara, S: "Epiplakin, a novel member of the plakin family originally identified as a 450-kDa human epidermal autoantigen : Structure and tissue localization."J Biol Chem. in press. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujiwara,S.Takeo,N.Otani,Y.Parry,D.A.D.Kunimatsu,M.: "Epiplakin, a novel member of the plakin family originally identified as a 450-kDa human epidermal autoantigen : Structure and tissue localization."J Biol Chem . (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Muro,N.Azuma,H.Onouchi,M.Kunimatsu,Y.Tomita,M.Sasaki,: "Autoepitopes on autoantigen CENP-A are restricted to the N-terminal region, which has no homology with histone H3"Clinical & Experimental Immunology. 120. 218-223 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Chiang KC,Goto S,Chen C,L,Lin CL,Lin YC,Kunimatsu M,: "Clusterin may be involved in rat liver allograft tolerance"Transplant Immunology. 8. 95-99 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Muro,M.Kunimatsu,etc: "Autoepitopes on autoantigen CENP-A are restricted to the N-terminal region, which has no homology with histone H3"Clinical & Experimental Immunology, in press. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kunimatsu,M.,Tada,T.,etc: "Activation of calpain in myocardial infarction: an immunohistochemical study using a calpain antibody raised agaist active site histidine-containing peptide"Cardiovascular Pathology. 8・1. 7-15. (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Saito,T.,Kunimatsu,M.,etc: "Protective effects of calpain inhibitor for prolonged hypothermic cardiac preservation"JPN J Thorac Cardiovasc Surg. 47・4. 145-152 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 佐々木實、国松己歳: "虚血・低酸素下における選択的脳神経細胞死並びにその制御に関する研究"臨床成人病. 29・10. 1391-1394 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.Fukuda,K.Harada,M.Kunimatsu, etc: "Postlschemic reperfusion Induces alpha-fodrin proteolysis by in-calpain in the synap tosome and nucleus in rat brain" Journal of Neurochemistry. 70巻6号. 2526-2532 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Hitomi,Y.Uchiyama,I.Ohkubo,M.Kunimatsu,M.Sasaki,etx: "Puriflcation and characterization of the active-site-mutated recombinant human mu-calpain expressed in baculovirus-infected insect cells" Biochem Biophys Res Commun. 6巻3号. 681-685 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Kondo,M.Mizokami,T.Nakano,M.Kumimatsu,etc: "Analysis of conserved ambisense sequences within GB virus C" Journal of Infectious Diseases. 178巻10号. 1185-1188 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.Sakuma,M.Yoshikawa,T.Yoshimata,M.Kunimatsu,etc: "a-Tocopherol protects the peroxidative modification of LDL to be recognized by LDL receptors" Journal of Nutritional Science and Vitaminology. 44巻5号. 697-703 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Kunimatsu,T.Tada,Y.Narita,T.Shearer,etc: "Activation of calpain in myocardial infarction : an immunohistochemical study using a calpain antibody raised agaist active site histidine-containing peptide" Cardiovascular Pathology. 8巻1号. 7-15 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] J.Fu, M.Kunimatsu, et.al: "MSP1-reactive T cells in individuals in malaria endemic Solomon area and in non-immune Japanease" Parasitology International. 46. 7-16 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Sasaki, M.Kunimatsu, et.: "Selective neuronal cell death induced by activation of mu-calpain under conditions of ischemia/reperfusion" Biomedical and Health Research. 15. 43-57 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 伊藤博隆, ら: "アレルギー性鼻炎における減感作療法の長期観察成績 -アンケート結果から-" 日鼻誌. 36. 35-39 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Tada, et.al.: "Membrane attack complex of complement and 20 Kda homologous restriction factor (CD59) in myocardial infarction" Virchows Arch. 430. 327-332 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi