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ENERGY METABOLISM-DEPENDENT CONTRAST REAGENT IN NEAR INFRARED REGION

Research Project

Project/Area Number 09558108
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

NOMURA Yasutomo  Res.Instit.Electro.Sci., Hokkaido Univ.Research Associate, 電子科学研究所, 助手 (80237883)

Co-Investigator(Kenkyū-buntansha) EGUCHI Hiroaki  Japan Schering Corp.Researcher, 基礎研究所, 研究員
INAGAKI Michihito  Japan Schering Corp.Researcher, 基礎研究所, 研究員
JIN Takashi  Res.Instit.Electro.Sci., Hokkaido Univ.Research Associate, 電子科学研究所, 助手 (80206367)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsRhodamine 800 / mitochondria / membrane potential / energy state / hepatocyte / myocardial cell / absorption / fluorescence
Research Abstract

It is an object of the present research to provide a method for determining the energy state of cell or tissue, more particularly, a method for determining energy state, which has a high transmittivity and therefore is capable of determining a thick tissue such as an organ.
As a results of intensive studies, there have been found that energy state of cell or tissue can be determined by bringing Rhodamine 800, which is specifically incorporated into cell mitochondria and shows varying absorption spectrum or fluorescence intensity ; that the changes in the absorbance can be appropriately detected by measuring the difference between absorbances at two different wavelengths respectively selected from a wavelength range of 730-850 nm and a wavelength range of 730-900 nm, preferably 730 nm and 800 nm ; and that Rhodamine 800 incorporated into mitochondria forms a complex within the mitochondria along the changes in mitochondrial membrane potential and affords different fluorescence spectra.
Ac … More cordingly, the present research provides the following.
(1) A method for determining an energy state of a cell or tissue, which comprises bringing Rhodamine 800 into contact with the cell and/or the tissue and measuring the difference between absorbances at two different wavelengths respectively selected from a wavelength region of 730-850 nm and a wavelength region of 730-900 nm, preferably 730 nm and 800 nm.
(2) A method for determining an energy state of a cell or tissue, which comprises bringing Rhodamine 800 into contact with the cell and/or the tissue and measuring fluorescence intensity caused by a complec formed within mitochondria.
(3) A method for (2) above, which further comprises fluorescent excitation at a wavelength selected from the range of 600-680 nm and measuring a fluorescence emission intensity at a wavelength selected from the range of 680-730 nm and different from the excitation wavelength, particularly, fluorescent excitation at 660 nm and measuring a fluorescence emission intensity at 692 nm.
(4) A method for determining an energy state of hepatocytes and/or myocardial cells by the method of (1) to (3) above.
(5) The method of (1) to (4) above, wherein the energy state of cell means an irreversible cell death (loss of membrane potential). Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Y. Nomura: "Charges in neural activity propagation in rot nippocampus following chemia-like conditions as : tudiod by optical recording" Neurosci.Res.Commun.24・2. 71-80 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M Nemoto: "Obserations of the activity-related optical intrinsic signals in rat somatosensory cortex" J Cereb.Blood Flow Metabol.19・3. 246-259 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y. Nomura: "Optical characterlzation of heme a+a^3 and copper of cytochrome oxidase in blood-free perfused brain" J.Neurosci.Meth.82. 135-144 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y. Nomura: "The reaction of copper in cytochrome oxidase with cytochromec in a rat brain in situ" Int.J.Neurosci.94. 205-212 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A. Matsunaga: "Energy dopendence of redox state of heme a+a^3 in cytochrome oxidase in perfused rat brain in sita" Am.J.Physiol.275・44. C1022-C1030 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Kawaji: "Flarin-containing monooxygenase mediated metabolism of benzydamine in perfased brain and liver" Biochim.Biophys.Acta. 1425. 41-46 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 田村 守: "麻酔 第47巻 増刊号 「近赤外分光法の基礎と臨床応用」" 克誠堂出版, 13 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] J.Sakanoue, K.Ichikawa, Y.Nomura, and M.Tamura: "Rhodamine 800 as a probe of energization of isolated rat liver mitochondria and hepatocytes" J.Biochem.121. 29-37 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Nomura, O.Hazeki, and M.Tamura: "Relationship between time resolved and non time resolved Beer Lambert law in turbid media" Phys.Med.Biol.42 (6). 1009-1022 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Nomura, A.Matsunaga and M.Tamura: "Optical characterization of heme a+a3 and copper of cytochrome oxidase in blood-free perfused brain" J.Neurosci.Meth.82. 135-144 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Matsunaga, Y.Nomura, M.Tamura, S.Kuroda, N.Yoshimura, and J.Nishihira: "Energy dependence of redox state of heme a+a3 in cytochrome oxidase in perfused rat brain in situ" Am.J.Physiol.275 (Cell Physiol.44). C1022-C1030 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Nomura, F.Fujii, A.Matsunaga, and M.Tamura: "The reaction of copper in cytochrome oxidase with cytochrome c in rat brain in situ" Int.J.Neurosci.94. 205-212 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Kawaji, M.Isobe, Y.Tochino, E.Takabatake, Y.Chikaoka, Y.Nomura, and M.Tamura: "Flavin-containing monooxygenase mediated metabolism of benzydamine in perfused brain and liver" Biochim.Biophys.Acta. 1425. 41-46 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Nemoto, Y.Nomura, C.Sato, M.Tamura, I.Koyanagi, K.Houkin, and H.Abe: "Observations of the activity-related optical intrinsic signals in rat somatosensory cortex from the viewpoint of dynamic changes in local hemoglobin concentration and oxygenation" J.Cereb.Blood Flow Metabol.19. 246-259 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Nomura, K.Kamibayashi, S.Takuma, and T.Akaike: "Changes in neural activity propagbation in rat hippocampus following ischemia-like conditions as studied by optical recording" Neurosci.Res.Commun.24. 71-80 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Nomura: "Changes in neural activity propagation in rat hippocampus following ischemia-like conditions as itudied by opticol recording" Neurosci.Res.Commun.24・2. 71-80 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Nemoto: "Observations of the activity-relateu Optical intrinsic signals in rat scinatosensory cortex" J.Cereb.Blood Flow Metabol.19・3. 246-259 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Nomura: "Optical characterization of heme a+o_3 and copper of cytochrome oxidase in blood-free perfused brain" J.Neurosci.Meth.82. 135-144 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Nomura: "The reaction of copper in cytochrome oxidase with cytochromec in a rat brain in situ" Int.J.Neurosci.94. 205-212 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.Matsunaga: "Energy dependence of redox state of heme a+o_3 in cytochrome oxidase in perfused rat brain in sita" Am.J.Physiol.275・44. C1022-C1030 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.Kawaji: "Flarin-containing monooxygenase mediated metabolism of benzydomine in perfased brain and liver" Biochim.Biophys.Acta. 1425. 41-46 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 田村 守: "麻酔第47巻増刊号「近赤外分光法の基礎と臨床応用」" 克誠堂出版, 13 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] J.Sakanoue: "Rhodamine 800 as a probe of energization of isolated rat live mitochondria and hepatocytes" J.Biochem.121. 29-37 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Nomura: "Relationship between time resolved and non time resolved Beer Lambert law in turbid media" Phys.Med.Biol. 42. 1009-1022 (1977)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Nomura: "Optical characterization of heme a+a_3 and copper of cytochrome oxidase in blood-free perfused brain" J.Neurosci.Meth.(accepted). (1988)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Nomura: "The reaction of copper in cytochrome oxidase with cytochreme C in rat brain in situ" Int.J.Neurosci.(in press). (1988)

    • Related Report
      1997 Annual Research Report
  • [Publications] O.Murillo: "Synthetic transmembrane channels:Functionol characterization using solubility calenlations,transport studies,and substituent effects" J.Am.Chem.Soc. 119. 5540-5549 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] E.Abel: "Unusually long open times,determined by planar bilayer conductances studies" Chem.Commun. 1145-1146 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 神 隆: "アルカリ金属イオンに応答する蛍光性カリックス[4]アレーンの分子設計とその蛍光特性「染料と薬品」" 化成品工業協会, 10 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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