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Development of optical recording system for cardiac electrical activities and metabolism

Research Project

Project/Area Number 09558114
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SAKAI Tetsuro  Tokyo Medical and Dental University School of Medicine, Associate Professor, 医学部, 助教授 (40153845)

Co-Investigator(Kenkyū-buntansha) KAMINO Kohtaro  Tokyo Medical and Dental University School of Medicine, Professor, 医学部, 教授 (40025630)
SATO Katsushige  Tokyo Medical and Dental University School of Medicine, Research Associate, 医学部, 助手 (80291342)
SATO Yoko (MOMOSE Yoko)  Tokyo Medical and Dental University, Lecturer, 大学院・医歯学総合研究科, 講師 (70251501)
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 1999: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1998: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1997: ¥5,500,000 (Direct Cost: ¥5,500,000)
Keywordselectrical activity / metabolism / optical recording / voltage sensitive dye / heart
Research Abstract

This project is aimed at developing a new optical recording system for the simultaneous recording of the electrical activity and the metabolism in the heart preparation. The system is composed of four main parts, including 1) optics and a recording chamber, 2) a detection system with a multiple channel recording system for the voltage-sensitive dye-recording of electrical activities, 3) the second detection system with a CCD camera for recording the metabolism of cardiac cells and 4) a computer for analyzing experimental data. The preparation chamber is placed on the stage of a microscope, in which a beam splitter is build, mounted on the vibration-isolation table. Two kinds of optical detection system are mounted to the microscope : a 16 X 16- or 12 X 12-element photodiode array for the extrinsic signals corresponding to the electrical activities, and a cooled CCD camera for the intrinsic fluorescence of NADH reflecting the metabolism of the cardiac cells. Data acquired using these detectors are transferred to a graphic workstation via LAN (local area network) to analyze the data visually. In the physiological experiments, right atrial preparations dissected from the adult rat heart are used. The preparations are stained with a merocyanine-rhodanine dye (NK2761), and a bathing medium containing 2,3-butanedione monoxime is used to reduce the contraction-related light scattering. Using this system, the relationship between the conduction pattern of the excitatory wave and the metabolic state of cardiac muscle is analyzed.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Sakai T.: "Optical Mapping of Conduction Patterns of Normal and Tachycardia-Like Excitations in the Rat Atrium"Japanese Journal of Physiology. 47. 179-188 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Momose-Sato Y.: "OPTICAL CHARACTERIZATION OF A NOVEL GABA RESPONSE IN EARLY EMBRYONIC CHICK BRAINSTEM"Neuroscience. 80. 203-219 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sato K: "Optical Mapping of Neural Responses in the Embryonic Rat Brainstem with Reference to the Early Functional Organization of Vagal Nuclei"Journal of Neuroscience. 15. 1345-1362 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakai T.: "A regional gradient of cardiac intrinsic rhythmicity depicted in embryonic cultured multiple hearts"Pflugers Archiv : European Journal of Physiology. 437. 61-69 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakai T.: "Optical monitoring of synaptic transmission in bullfrog sympathetic ganglia using a voltage-sensitive dye"Neuroscience Letters. 242. 1-4 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Obaid A.L.,: "Spatiotemporal Patterns of Activity in an Intact Mammalian Network with Single-Cell Resolution : Optical Studies of Nicotinic Activity in an Enteric Plexus"Journal of Neuroscience. 19. 3073-3093 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakai T, Hirota A, Momose-Sato Y, Sato K, Kamino K.: "Optical mapping of conduction patterns of normal and tachycardia-like excitations in the rat atrium."Jpn. J. Physiol.. 47. 179-188 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Momose-Sato Y, Sato K, Hirota A, Sakai T, Yang X-S, Kamino K.: "Optical characterization of a novel GABA response in early embryonic chick brainstem."Neuroscience. 80. 203-219 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sato K, Momose-Sato Y, Hirota A, Sakai T, Kamino K.: "Optical mapping of neural responses in the embryonic rat brainstem with reference to the early functional organization of vagal nuclei."J. Neurosci.. 18. 1345-1362 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakai T, Momose-Sato Y, Sato K, Hirota A, Kamino K.: "Optical monitoring of synaptic transmission in bullfrog sympathetic ganglia using a voltage-sensitive dye."Neurosci. Lett.. 242. 1-4 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakai T, Yada T, Hirota A, Komuro H, Kamino K.: "A regional gradient of cardiac rhythmicity depicted in embryonic cultured multiple-hearts."Pflugers Archiv : Eur. J. Physiol.. 437. 61-69 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Momose-Sato Y, Komuro H, Hirota A, Sakai T, Sato K, Kamino K.: "Optical imaging of the spatiotemporal patterning of neural responses in the embryonic chick superior cervical ganglion."Neuroscience. 90. 1069-1083 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Obaid A L, Koyano T, Lindstrom J, Sakai T, Salzberg B M: "Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution : Optical studies of nicotinic activity in an enteric plexus."J. Neurosci.. 19. 3073-3093 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Momose-Sato, Y.: "OPTICAL IMAGING OF THE SPATIOTEMPORAL PATTRERNING OF NEURAL RESPONSES IN THE EMBRYONIC CHICK SUPERIOR CERVICAL GANGLION"Neuroscience. 90・3. 1069-1083 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Obaid, A.L.: "Spatiotemporal Patterns of Activity in an Intact Mammalian Network with Single-Cell Resolution: Optical Studies of Nicotinic Activity in an Enteric Plexus"Journal of Neuroscience. 19・8. 3073-3093 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sato,K.: "Optical mapping of neural responses in the embryonic rat brainstem with reference to the early functional organization of vagal nuclei." Journal of Neuroscience. 18. 1345-1362 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sakai,T.: "Optical monitoring of synaptic transmission in bullfrog sympathetic ganglia using a voltage-sensitive dye." Neuroscience Letters. 242. 1-4 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sakai,T.: "A regional gradient of cardiac rhythmicity depicted in embryonic cultured multiple-hearts." Pflugers Archiv:European Journal of Physiology. 437. 61-69 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Momose-Sato,Y.: "Optical imaging of the spatiotemporal patterning of neural responses in the embryonic chick superior cervical ganglion." Neuroscience. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Obaid,A.L.: "Spatio-temporal patterns of activity in an intact mammalian network with single cell resolution:Optical studies of nicotinic activity in an enteric plexus." Journal of Neuroscience. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sakai,T.: "Optical mapping of conduction patterns of normal and tachycardia-like excitations in the rat atrium." Jpanese Journal of Physiology. 47. 179-188 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Momose-Sato,Y.: "Optical characterization of a novel GABA response in early embryonic chick brainstem." Neuroscience. 80. 203-219 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sato,K.: "Optical mapping of neural responses in the embryonic rat brainstem with reference to the early functional organization of vagal nuclei" Journal of Neuvoscieuce. 18. 1345-1362 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sakai,T.: "Optical monitoring of synaptic transmission in bullfrog sympathetic ganglia using a voltage-sensitive dye." Neuvoscieuce Letters. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2021-10-12  

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