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Development of bioresorbable porous sintered apatities as bone substitutes

Research Project

Project/Area Number 09558124
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionAsahi University

Principal Investigator

DOI Yutaka  Asahi university, School of Dentistry, Associate Professor, 歯学部, 助教授 (40116067)

Co-Investigator(Kenkyū-buntansha) ADACHI Masanori  Asahi university, School of Dentistry, Leutrue, 歯学部, 講師 (60076057)
GOTO Takayasu  Asahi university, School of Dentistry, Lecture, 歯学部, 講師 (30121320)
MORIWAKI Yutaka  Asahi university, School of Dentistry, Professor, 歯学部, 教授 (90028738)
KAMEMIZU Hideo  Asahi university, School of Dentistry, Assistant, 歯学部, 助手 (00152877)
WAKAMATSU Nobukazu  Asahi university, School of Dentistry, Assistant, 歯学部, 助手 (00158594)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥11,400,000 (Direct Cost: ¥11,400,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1997: ¥7,000,000 (Direct Cost: ¥7,000,000)
KeywordsCarbonate Apatite / Porous / Bone Substitutes / Bioresorbable / Osteoblasts / Osteoclasts / Biocimpatibility / Biomaterials / 焼結 / 骨アパタイト
Research Abstract

The usefulness of sintered carbonate apatites (CAp) as bone substitutes were investigated in vitro and in vivo. For in vitro evaluation, we used a rat bone marrow cell culture. In this cell culture, we also used sintered hydroxyapatite (HAp), β-tricalcium phosphate ( β-TCP), magnesium-containing β-TCP (Mgβ-TCP) and Hap(70wt%)-Mg, β-TCP(30wt%) substrates for comparison and the adhesion, proliferation, and differentiation of osteoblastic cells on these substrates including standard plastic culture plate substrates were investigated. At 7-day culture, parameters such as the number of cells adhered, DNA contents, alkaline phosphatase activity, calcium and phosphate contents originating from the calcified nodules were significantly higher with Cap substrates than with the plastic culture plate substrates. No great significant differences were noted among Hap, β-TCP, Mg β-TCP, Hap-Mg β-TCP and Cap in the parameters, though β-TCP tended to show smallest alkaline phosphatese activity with the … More number of cells being almost the same with the other calcium phosphatase activity with the number of cells being almost the same with the other calcuim phosphate substrates. At 14-day culture, the parameters with the plastic culture plate substrates tended to become close to those of the calcuim phosphate substrates including CAp substrates. Neverless, SEM micrographs clearly suggested that osteoblasitc cells appeared much more active and more proliferated on CAp, HAp, HAp-Mgβ-TCP substrates than on plastic plate, β-TCP and Mgβ-TCP substrates. For the latter three substrates, the calcium and phosphate contents of calcified nudules formed during culture were found to be smaller.
For in vivo evaluation, sintered carbonate apatites in porous form with each particle size being in the range of 300-500μm (coarse particles) and less than 200μm (fine particles) were implanted in rat skull defects. Micro-focus X-ray computed tomography showed that bone hardly formed in the defects filled with no materials at least till 3 weeks after implantation but in the defects filled with the materials bone formed by two weeks. Staining of sectioned specimens by TRAP showed that there were many TRAP-positive cells around the resorbing material, suggesting that the resorption of the sintered carbonate apatite particles is, in part, similar to osteoclastic bone resorption. At 4, 6 and 8 weeks after implantation, the new bone contained osteoclastic and was a mineralized and dense as the host bone. These findings suggest that sintered carbonate apatites would be useful as bioresorbable bone substitutes. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] 幸田起英: "炭酸含有アパタイトの焼結-pHスタットによる初期溶解速度-"歯科材料・器械. 17. 152-158 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yutaka Doi: "Sintered Carbonate Apatites as Bioresorbable Bone Substitutes"Journal of Biomedical Materials Research. 39. 603-610 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yutaka Doi: "Bioresorbable bone substitute materials made of sintered carbonate apatites"Bioceramics. 11. 323-326 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tadanori Kajimoto: "Resorption of collagen-carbonate apatite-cimplex in skull defects"Bioceramics. 395-398 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yutaka Doi: "Osteoclastic responses to various calcium phosphates in cell sultures"Journal of Biomedical Materials Research. 41(3). 424-433 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshiaki Shibutani: "Use of glass slides coated with apatite-collagen complexes for measurement of osteclastic resorption activity"Journal of Biomedical Materials Research. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 土井豊: "セラミックス系素材の現状と課題「アパタイトセメント」"中部科学技術センター(広域研究会A). 5 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 土井豊: "バイオミネライゼーションと生体材料"ブイオミメティック材料工学研究会. 13 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Doi: "Biomineralization and Biomaterials"Biomimetic Mater. Engineering. 37-49 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Doi, T.Shibutani, Y.Moriwaki, T.Kajimoto and Y.Iwayama: "Sintered carbonate apatites as bioresorbable bone substitutes"J.Biomed.Mater.Res. 39(4). 139-143 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Koda, Y.Doi, Y.Shimizu, M.Adachi, N.Wakamatsu, T.Goto, M.Nishikawa, H.Kamemizu and Y.Moriwaki: "Sintering of carbonante-containing apatites - Dissolution behavior investigated with a PH-stat-"J.Jap.Soc.Dent.Mater.Dev. 17(3). 152-158 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Doi, S.Saku, H.Iwanaga, T.Kajimoto, Y.Moriwaki, K.Yamamoto, T.Shibutani, Y.Iwayama, Y.Mizuta and Y.Ikesa: "Bioresorbable bone substitute materials made of sintered carbonate apatites"Bioceramics. 11. 323-326 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Kajimoto, T.Tanake, K.Knodou, S.Saku, K.Yamamoto and Y.Doi: "Resorption of collagen-carbonate apatite-complex in skull defects"Bioceramics. 11. 395-398 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Doi, K.Iwanaga, T.Shibutani, Y.Moriwaki and Y.Iwayama: "Osteoclastic responses to various calcium phosphates in cell sultures"J.Biomed.Mater.Res.. 41(3). 424-433 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Shibutani, K.Iwanaga, K.Imai, M.Kitago, Y.Doi and Y.Iwayama: "Use of glass slides coated with apatite-collagen complexes for measurement of osteclastic resorption activity"J.Biomed.Mater.Res.. In press. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 幸田起英: "炭酸含有アパタイトの焼結―pHスタットによる初期溶解速度―"歯科材料・器械. 17. 152-158 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yutaka Doi: "Sintered Carbonate Apatites as Bioresorbable Bone Substitutes"Journal of Biomedical Materials Research. 39. 603-610 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yutaka Doi: "Bioresorbable bone substitute materials made of sintered carbonate apatites"Bioceramics. 11. 323-326 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tadamori Kajimoto: "Resorption of collagen-carbonate apatite-cimplex in skull defects"Bioceramics. 11. 395-398 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yutaka Doi: "Osteoclastic responses to various calcium phosphates in cell sultures"Journal of Biomedical Materials Research. 41(3). 424-433 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tosiaki Shibutani: "Use of glass slides coated with apatite-collagen complexes for measurement of osteclastic resorption activity"Journal of Biomedical Materials Research. (In press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 土井豊: "セラミックス系素材の現状と課題 アパタイトセメント"中部科学技術センター(広域研究会A). 5 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 土井豊: "バイオミネライゼーションと生体材料"ブイオミメティック材料工学研究会. 13 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 幸田 起英: "炭酸含有アパタイトの焼結-pHスタットによる初期溶解速度-" 歯科材料・器械. 17. 152-158 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yutaka DOI: "Sintered Carbonate Apatites as Bioresorbable Bone Substitutes." Journal of Biomedical Materials Reserch. 39. 603-610 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yutaka DOI: "Bioresorbable bone substitute materials made of sintered carbonate apatites." Bioceramics. 11. 323-326 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tadamori Kajimoto: "Resorption of collagen-carbonate apatite-cimplex in skull defects." Bioceramics. 11. 395-398 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yutaka DOI: "Development of a new calcium phosphate oement that contains sodium calcium phosphate." Bioceramics. 12(予定). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 土井 豊: "セラミックス系素材の現状と課題「アパタイトセメント」" 中部科学技術センター(広域研究会A), 5 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 土井 豊: "バイオミネライゼーションと生体材料" ブイオミメティック材料工学研究会, 13 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 幸田 起英: "炭酸含有アパタイトの焼結-弱酸中での焼結体の溶解挙動-" 歯科材料・器械. 16. 107-113 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 幸田 起英: "炭酸含有アパタイトの焼結-pHスタットによる初期溶解速度-" 歯科材料・器械. 17(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yutaka DOI: "Sintered Carbonate Apatites as Bioresorbable Bone Substitutes." Journal of Biomedical Materials Research. 39. 603-610 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yutaka DOI: "Sintered Carbonate Apatites as Bone Substitutes." Cells and Materials. 7(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 土井豊: "ハイドロキシアパタイト I" 基盤研究センター(バイオセラミックスの開発および適用技術に関する調査), 20 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 土井豊: "セラミックス系素材の現状と課題「アパタイトセメント」" 中部科学技術センター(広域研究会A), 5 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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