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Multicellular Behavior of Bacteria and Dynamics of Their Colony Formation

Research Project

Project/Area Number 09640471
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 物性一般(含基礎論)
Research InstitutionChuo University

Principal Investigator

MATSUSHITA Mitsugu  Chuo University, Dept.of Physics, Professor, 理工学部, 教授 (20091746)

Co-Investigator(Kenkyū-buntansha) MATSUYAMA Tohey  Niigata University, School of Medicine, Associate Professor, 医学部, 助教授 (00047200)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsbacteria / colony / pattern formation / Bacillus subtilis / Proteus mirabilis / interface growth / self-affinity / concentric-ring pattern / DBM(密集分技)パターン / 細菌
Research Abstract

We have studied the, growth mechanism and morphological change, in colony formation of bacteria from the viewpoint of physics of pattern formation. The colony differs in size, form, and color according to bacterial species. It also changes its form sensitively with the variation of environmental conditions. This implies that although usual bacteria such as Escherichia coli are regarded as single cell organisms, they never make, their colony independently and randomly but somehow collaborate multicellularly. We have thus tried to extract some simple, and universal behaviors in growth from such complex bacterial systems.
Here we varied only two parameters to investigate the colony growth; concentrations of nutrient CィイD2nィエD2 and agar CィイD2aィエD2 in a thin agar plate as the incubation medium. Other parameters specifying experimental conditions such as temperature were kept constant. We mainly used a typical bacterial species Bacillus subtilis. Otherwise the experimental procedures are standard. It was found that coloniels show characteristic patterns in the specific regions of values of CィイD2nィエD2 and CィイD2aィエD2 in the morphological diagram and the patterns change drastically from one region to another. They were classified into five types; fractal DLA-like, compact Eden-like, concentric ring-like, simple disk-like and densely branched DBM-like. We have experimentally elaborated characteristic properties for each of these colony patterns.
We have also done the same experiments for a bacterial species Proteus mirabilis. This species has been well-known for more than a century to form macroscopically almost perfect concentric-ring like, colonies with approximately equal spacings on the surface of semi-solid substrate such as an agar plate. We have elucidated macroscopic and phenomenological mechanism for the repeated interface growth of the concentric-ring like colonies.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] J. Wakita: "Self-affinity for the Growing Interface of Bacterial Colonies"J. Phys. Soc. Jpn. 66. 67-72 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Kawasaki: "Modeling Spatio-Temporal Patterns Generated by Bacillus subtilis"J. theor. Biol. 188. 177-185 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J. Wakita: "Experimented Investigation on the Formation of Dense-Branching-Morphology-like Colonies in Bacteria"J. Phys. Soc. Jpn. 67. 3630-3636 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Itoh: "Periodic Pattern Formation of Bacterial Colonies"J. Phys. Soc. Jpn. 68. 1436-1443 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Matsushita: "Formation of Colony Patterns by a Bacterial Cell Population"Physica A. 274. 190-199 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Matsuyama: "Dynamic Aspects of Structured Cell Population in a Swarming Colony of Proteus mirabilis"J. Bacteriol.. 182. 385-393 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J. Wakita et al.: "Self-Affinity for the Growing Interface of Bacterial Colonies"J. Phys. Soc. Jpn.. 66. 67-72 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Kawasaki et al: "Modeling Spatio-Temporal Patterns Generated by Bacillus subtilis"J. theor. Biol.. 188. 177-185 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J. Wakita et al.: "Experimental Investigation on the Formation of Dense-Branching-Morphology-like Colonies in Bacteria"J. Phys. Soc. Jpn.. 67. 3630-3636 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Itoh et al.: "Periodic Pattern Formation of Bacterial Colonies"J. Phys. Soc. Jpn.. 68. 1436-1443 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Matsuyama et al.: "Coments on 'Classification and Genetic Characterization of Pattern Forming Bacilli'"Mol. Microbiol.. 31. 1278-1279 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Matsushita et al.: "Formation of Colony Patterns by a Bacterial Cell Population"Physica A. 274. 190-199 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Matsuyama et al.: "Dynamic Aspects of Structured Cell Population in a Swarming Colony of Proteus mirabilis"J. Bacteriol.. 182. 385-393 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Matsuyama: "Comments on 'Classification and genetic characterization of pattern-forming Bacilli'"Mol.Microbiol.. 31. 1278-1279 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Itoh: "Periodic pattern formation of bacterial colonies"J.Phys.Soc.Jpn.. 68・4. 1436-1443 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Matsushita: "Formation of colony patterns by a bacterial cell population"Physica A. 274. 190-199 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Matsuyama: "Dynamic aspects of the structured cell population in a swarming colony of Proteus mirabilis"J.Bacteriol.. 182・2. 385-393 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] J.Wakita: "Experimental Investigation on the Formation of Dense-Branching-Morphology-like Colonies in Bacteria" J.Phys.Soc.Jpn.67. 3630-3636 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Matsuyama: "Comments on ‘Classification and Genetic Characterization of Pattern-forming Bacilli'Mol. Microbiol. 27:687-703 (1998)" Mol.Microbiol.(発表予定).

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Itoh: "Periodic Pattern Formation of Bacterial Colonies" J.Phys.Soc.Jpn.(発表予定).

    • Related Report
      1998 Annual Research Report
  • [Publications] J.Wakita: "Self-Affinity for the Growing Interface of Bacterial Colonies" J.Phys.Soc.Jpn.66. 67-72 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] O.Moriyama: "Fractal Structure and Statistics of Growing Patterns with Competition" Fractals. 5. 247-265 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Kawasaki: "Modeling Spatio-Temporal Patterns Generated by Bacillus subtilis" J.theor.Biol. 188. 177-185 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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