| Budget Amount *help |
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Research Abstract |
In this study, metal complexes of 1) bleomycin (Blm) and deglycopepromycin (Dpe), 2) acridine-polyamin, 3) histidine-containing peptides, 4) vilogen-porphyrin have been investigated on the novel binding structures and the reactions on DNA. The results are listed below.
1) Fe(III)Blm binds DNA with two different orientations and NO in ON-Fe(II)Blm is held rigidly in place and the motion is severely restricted on the DNA. The low-spin Fe(III)Dpe changes irreversibly to high-spin species on DNA. Cu(II)Dpe cannot bind stereo specifically on DNA.
2) Some platinum complexes of acridine-polyamines show more intense cytotoxicity than cis-platin. For the copper(II) complexes, it was found by DNA-fiber ESR spectroscopy that the coordinating moiety of some of the complexes is moving vigorously at room temperature.
3) Cu(II) complex of L-histidine forms an aggregate stereospecifically bound on DNA. Cu(II) complexes of XィイD2aaィエD2-Gly-His (XィイD2aaィエD2=Gly, Lys, Arg) binds in the minor groove of B-form DNA. The sequence specific cleaving reactions by corresponding Ni(III) complexes were discussed in terms such a binding structure.
4) Cu(II) complex of a meso-substituted tetra-viologen porphyrin binds DNA so strongly that it bridges double helical DNA, while the mono-substituted porphyrin complex is so unstable that it decomposes on DNA to form a Cu(II) complex with the unknown fragment derived from the porphyrin.
The above results have been published in several journals or presented at the international and domestic symposiums as shown in the Research Report.
|
