| Project/Area Number |
09640725
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
分離・精製・検出法
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
KITAOKA Yoshinori Research Reactor Institute, KYOTO UNIVERSITY Assistant Professor., 原子炉実験所, 助教授 (00027426)
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| Co-Investigator(Kenkyū-buntansha) |
北岡 祥伯 京都大学, 原子炉実験所, 助教授 (00027426)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Zone Electrophoresis / p-Boronophenylalanine / Oxalate / Citrate / lsocitrate / Tyrosine / p-Boronoserine / Phenylalanine / アドレナリン / ピナコール / ゾーン電気泳動 / イソクエン酸 / 錯形成反応 / フルクトース / アコチニン酸 / ホウ酸 / エチレングリコール / プロピレングリコール / トリメシン酸 |
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| Research Abstract |
The observed migration on zone electrophoresis is a summation of capillary action, electro-osmotic flow and electrophoretic movement. Electrophoretic behavior of p-boronophenylalanine (p-BPA) and its related compounds was studied under various electrophoresis conditions.
1. The migration distances of p-BPA, m-BPA and p-boronophenylserine (p-BPS) in acidic oxalate supporting solutions were compared with those of usual amino acids [Tyrosine (Tyr), Phenylalanine (Phe) and 3,4-dihydroxy phenylalanine]. p-BPA, m-BPA and p-BPS showed unusual anionic movements. p-BPA was specifically isolated from a mixture of Tyr, Phe and p-BPA by using a oxalate supporting solution.
2. Zone electrophoretic behavior of p-BPA was studied by using aqueous ethylene glycol containing sodium chloride. Anionic movement of p-BPA was not observed in any supporting solution. The electric current dropped sharply with decreasing concentration of sodium chloride in solutions.
3. p-BPA did not migrate to the anion side in acotinic or trimesic acid supporting solution. The complexation of p-BPA was found in isocitric or citric acid supporting solution.
4. The stability of complexes of p-BPA against the pH-values of supporting solution was studied by the electrophoresis.
5. Effect of the various supports (pure cellulose paper, acetate paper, phosphate paper) on migration of p-BPA was investigated.
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