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Analysis of molecular mechanisms involved in the evolution of plant morphology

Research Project

Project/Area Number 09640736
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionNARA INSTITUTE OF SCIENCE AND TECHNOLOGY

Principal Investigator

KYOZUKA Junko  Nara Institute of Science & Technology, Bio Science, バイオサイエンス研究科, 助教授 (90273838)

Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
KeywordsRice / Floral organ development / ABC model / Homeotic mutation / ホメオティック遺伝子 / 形態形成 / MADSボックス遺伝子 / 穂 / 形態進化
Research Abstract

The floral morphology of grass species is distinct from typical dicot plants. In order to achieve a better understanding of the molccular basis for this diversion, we isolated RAP1A, RAP1B and RAG, putative rice homologs for the Arabidopsis class A gene APETALA1(AP1) and class C gene AGAMOUS(AG), respectively. Expression patterns of RAP1A.RAG, and OsMADS2, a rice homolog of the class B gene, were analyzed by in situ hybridization. RAP1A mRNA was expressed in the apical region of the floral meristem at an early stage of spikelet development, and then its expression was localized in developing lemma, palea and lodicules. OsMADS2 transcript was first observed in the region where stamen primordia are formed. Soon after, OsMADS2 mRNA appeared in the lodicule primordia as well as stamen primordia and this RNA accumulation pattern persisted till late stages of floral development. The expression of RAG was observed in stamens and pistils of wild type young spikelets. These RNA accumulation pat … More terns are mostly similar to those of Arabidopsis class A, B, C genes supporting the notion that the ABC model may be extended to rice.
In order to clarify the evolutionary relationship between grass sterile organs, such as lodicules, the lemma and the palea, and the dicot floral organs, we expressed RAG, a rice (Oryza sativa L.) AGAMOUS(AG) ortholog, in rice plants under the control of an Actin I promoter.As a consequence of the ectopic expression of the RAG, lodicules were homeotically transformed into stamens. In total, the transformation of lodicules to staminoid organs was observed in 18 out of 26 independent transgenic lines. In contrast to the almost complete transformation occurred in lodicules, none of the transgenic plants exhibited any morphological alterations in the palea or the lemma. Our results confirmed the prediction that the lodicule is an equivalent of a dicot petal and that the ABC model can be applied to rice at least for organ specification in lodicules and stamens. On the other hand, the absence of morphological alterations in the palea or the lemma raised the possibility that the simple application of the ABC model to structures outside the lodicules may not be valid. Less

Report

(5 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Kyozuka J: "Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A,B,C genes."Plant Cell Physiol.. 41. 710-718 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyozuka J: "Down-regulation of RFL, the FLO/LFY homolog of rice, accompanied with panicle branch initiation."Proc.Natl.Acad.Sci.USA. 95. 1979-1982 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyozuka J: "Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A, B, C genes"Plant Cell Physiol.. 41. 710-718 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyozuka J: "Down-regulation of RFL, the FLO/LFY homolog of rice, accompanied with panicle branch initiation."Proc.Natl.Acad.Sci.USA. 95. 1979-1982 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kyozuka,Kobayashi,Morira,Shimamoto: "Conservation of the ABC model in Flower davalopment of vice"Plant and cell Physiology. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Junko Kyozuka: "Role of RFL, the FLOILFY homolog of rice in panicle development." Flowering Newsletter. 26. 11-18 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Junko Kyozuka: "Molecular Biology of Rice(Shimamoto.K.編集)" シュプリンガー社, 301 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Junko Kyozuka: "Down-regulation of RFL,the FLO/LFY homolog of rice,accompanied with panicle branch initiation" Proc.Natl.Acad.Sci.USA. 95. 1979-1982 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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