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Genetic and molecular analyzes of bacterial flagellum which acts as a transcription-controlling apparatus

Research Project

Project/Area Number 09640737
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

KUTSUKAKE Kazuhiro  Hiroshima University Faculty of Applied Biological Science Professor, 生物生産学部, 教授 (90143362)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsBacterial flagella / Transcriptional control / Signal transduction / Anti-sigma factor / Flagellar hook / Protein export / Export switch / Hook length control / 輸送ゲート
Research Abstract

During flagellar morphogenesis in Salmonella typhirnurium, the genes involved in filament assembly are expressed fully only after completion of hook assembly. This coupling of gene expression to morphogenesis is achieved by exporting the flagellum-specific anti-sigma factor, FlgM, out of the cell through the mature hook-basal body structure. Therefore, the flagellum-specific export apparatus must be able to sense the assembly state of the flagellar structure and to turn on FlgM export at the specific stage of hook assembly. In this study, this export switching machinery was dissected at a molecular level. The main conclusions are as follows. (1) The export gate for FlgM is double locked by FlhB and RflH proteins before completion of hook assembly. (2) These locks are opened in the mutants which produce abnormally elongated rods lacking hooks. This indicates that the monitor system of hook length measures the length of the rod plus the hook. (3) The FlgM gate is also involved in the export control of the late flagellar proteins, FlgK, FlgL, FliD, and FliC.FlhB is also responsible for shutting-off process of the export of hook protein after completion of hook assembly. (4) Mutants in which the RflH lock is opened constitutively were isolated. Genetic analysis of these mutants suggested that at least two proteins are involved in the opening process of the RflH lock. (5) The FlhB lock is opened at the early stage of hook assembly, whereas the RflH lock is opened upon completion of hook assembly.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kutsukake,K.: "Hook-length control of the export-switching machinery involves a double-locked gate in Salmonella typhimurium flagellar morphogenesis" Journal of Bacteriology. 179(4). 1268-1273 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kutsukake,K.: "Autogenous and global control of the flagellar master operon,flhD,in Salmonella typhimurium" Molecular and General Genetics. 254(4). 440-448 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ide,N.: "Identification of a novel Escherichia coli gene whose expression is dependent on the flagellum-specific sigma factor,FliA,but dispensable for motility development" Gene. 199(1). 19-23 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nambu,T.: "Peptidoglycan-hydrolyzing activity of the FlgJ protein,essential for flagellar rod formation in Salmonella typhimurium" Journal of Bacteriology. 181(5). 1555-1561 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikebe,T.: "Structure and expression of the fliA operon of Salmonella typhimurium" Microbiology. 145印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Minamino,T.: "Substrate specificity switching of the flagellum-specific export apparatus during flagellar morphogenesis in Salmonella typhimurium" Bioscience,Biotechnology,and Biochemistry. 63印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 沓掛和弘(分担執筆): "分子細胞生物学辞典" 東京化学同人(村松正実他編集), 1025 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 沓掛和弘(分担執筆): "化学と生物 35巻" 学会出版センター(日本農芸化学会編集), 884 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kutsukake, k.: "Hook-length control of the export-switching machinery involves a double-locked gate in salmonella typhimurium flagellar morphogenesis" Journal of Bacteriology. 179(4). 1268-1273 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kutsukake, K.: "Autogenous and global control of the flagellar master operon, flhD,in Salmonella typhimurium" Molecular and General Genetics. 254(4). 440-448 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ide, N.and Kutsukake, K.: "Identification of a novel Escherichia coli gene whose expression is dependent on the flagellum-specific sigma factor, FliA,but dispensable for motility development" Gene. 199(1). 19-23 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nambu, T., Minamino, T., Macnab, R.M., and Kutsukake, K.: "Peptidoglycan-hydrolyzing activity of the FlgJ protein essential for flagellar rod formation in Salmonella typhimurium" Journal of Bacteriology. 181(5). 1555-1561 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikebe, T., Iyoda, S., and Kutsukake, K.: "Structure and expression of the fliA operon of Salmonella typhimurium" Microbiology. 145 : (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Minamino, T., Doi, H., and Kutsukake, K.: "Substrate specificity switching of the flagellum-specific export apparatus during flagellar morphogenesis in Salmonella typhimurium" Bioscience, Biotechnology, and Biochemistry. 63 : (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nambu,T.: "Peptidoglycan-hydrolyzing activity of the Flgj protein,essential for flagellar rod formation in Salmonella typhimurium" Journal of Bacteriology. 181(5). 1555-1561 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ikeda,T.: "Structure and expression of fliA operon of Salmonella typhimurium" Microbiology. 145(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kutsukake,K.: "Identification of regulatory genes involved in transcriptional control of the class2 flagellar operons in Salmonella typhimurium" Genes&Genetic Systems. 73(6). 418 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nambu,T.: "Role of the FlgJ protein in the flagellar rod formation of Salmonella typhimurium" Genes&Genetic Systems. 73(6). 418 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ide,N.: "Identification of a novel Escherichia coli gene whose expression is depdndent on the flagellum-specific sigma factor, FliA, but dispensable for motility development" GENE. 199. 19-23 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 沓掛 和弘: "転写制御装置として機能する細菌鞭毛" 化学と生物. 35. 828-834 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Minamino,T.: "Role of the FlhA protein in the export process of flagellar proteins in Salmonella typhimurium" GENES&GENETIC SYSTEMS. 72. 380 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kubo,T.: "Role of the FlgD protein in the flagellar hook formation of Salmonella typhimurium" GENES&GENETIC SYSTEMS. 72. 380 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamoto,S.: "Role of the FliS protein in the flagellum-specific export process of Salmonella typhimurium" GENES&GENETIC SYSTEMS. 72. 381 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ikebe,T.: "Role of the fliZ gene in the transcriptional control of the flagellar regulon of Salmonella typhimurium" GENES&GENETIC SYSTEMS. 72. 381 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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