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Structures and functions of band boundaries of mammalian chromosomes

Research Project

Project/Area Number 09640740
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionNATIONAL INSTITUTE OF GENETICS

Principal Investigator

IKEMURA Toshimichi  National Inst. of Genetics. Dept. of Popul. Genet., Professor, 集団遺伝研究系, 教授 (50025475)

Co-Investigator(Kenkyū-buntansha) 天前 豊明  国立遺伝学研究所, 集団遺伝研究系, 助手 (70270460)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsGC content / Replication timing / Chromosome band boundary / Centromere / Replication pausing / Polypurine / polypyrimidine / MHC region / Xinactivation center / 染色体バンド / バンド境界 / X不活化中心
Research Abstract

Band zones of mammalian chromosome are associated with various genome characteristics such as DNA replication timing during S phase and long-range GC% mosaic structures. Therefore, band boundaries are thought to correspond to transition regions for replication timing and GC% levels, In this study, to identify replication switch point precisely, we determined replication timing in MHC and its surrounding non-MHC regions in and around XIC (X inactivation center), and in and around PAB (pseudoautosomal boundary), after chromosome walking and sequencing ; then correlation with GC% levels and chromosome band zones was examined. Concordant transitions for replication timing and GC% level were found in the junctions between MHC classes II and III, between class I and its telomeric non-MHC, and between class II and its centromeric non-MHC.The junction between class I and its telomeric non-MHC was assigned cytogenetically as the border between 6p21.3 and p22.1. The replication switch was also found in the 3' portion of XIST locus in XIC, that is thought to correspond to the border between Xql3.2 and q13.3, Within these junction regions, there are characteristic sequences including polypurine/polypyrimidine tracts with triplex-forming potential Triplexes are known to occur readily in polypurine/polypyrimidine sequences and to pause replication-fork movement in vitro. Using immunostaining with antitriplex antibodies and non-denatured FISH 'with polypurine/polypyrimidine probes, triplexes were found to be sequence-specificaly associated spatially with individual centromeres in the human interphase nucleus.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] 大野みずき: "Non-B DNA structures spatially and sequence-specifically associated with individual centromeres in the human interphase nucleus." Chromosomes Today. 13(in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 池村淑道: "Genome and subnuclear organization of non-B forming DNAs in mammalian chromosomes." Cytogenet Cell Genet. 81. 101-102 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 椎名崇: "Nucleotide sequencing analysis of the 146 kb segment around the IKBL and MICA genes at the centromeric end of the HLA class 1 region." Genomics. 47. 372-382 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 生田智樹: "Structural analysis of mouse tenascin-X: evolutionary aspects of reduplication of FNIII repeats in the tenascin gene family." Gene. 217. 1-13 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 大野みずき: "Triplex-forming DNAs in the human interphase nucleus visualized in situ by polypurine/polypyrimidine DNA probes and anti-triplex antibodies. Triplex-forming DNAs are spatially and sequence-specifically associated with distinct centromeres." submitted.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 渡辺良久: "Borders of early and late replication zones are present in and around the human X-inactivation center(XIC): correlation with chromosome band zones." submitted.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 天前豊明: "Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complex." Mol.Cell.Biol.17. 4043-4050 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 菅谷公彦: "Gene organization of human NOTCH4 and (CTG)n polymorphism in this human counterpart gene of mouse proto-oncogene Int3." Gene. 189. 235-244 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 安藤麻子: "Triplet repeat polymorphism in the NOTCH4 gene within the human major histocompatibility complex in a healthy population and patients with a salivary gland tumor in Japan." Tissue Antigens. 50. 66-70 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 安藤麻子: "Triplet repeat polymorphism within the NOTCH4 gene located near the junction of the HLA class II and class III regions in narcolepsy." Tissue Antigens. 50. 646-649 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mizuki ohn: "Non-B DNA structures spatially and sequence-specifically associated with individual centromeres in the human interphase nucleus." Chromosomes Today. 13(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshimichi Ikemura: "Genome and subnuclear organization of non-B forming DNAs in mammalian chromosomes." Cytogenet Cell Genet. 81. 101-102 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takasi Shiina: "Nucleotide sequencing analysis of the 146 kb segment around the IKBL and MICA genes at the centromeric end of the HLA class 1 region." Genomics. 47. 372-338 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tomoki Ikuta: "Structural analysis of mouse tenascin-X : evolutionary aspects of reduplication of FNIII repeats in the tenascin gene family." Gene. 217. 1-13 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyoaki tenzen: "Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complex." Mol.Cell, Biol.17. 4043-4050 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kimihiko Sugaya: "Gene organization of human NOTCH4 and (CTG)n polymorphism in this human counterpart gene of mouse proto-oncogene Int3." Gene. 189. 235-244 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Asako Ando: "Triplet repeat polymorphism in the NOTCH4 gene within the human major histocompatibility complex in a healthy population and patients with a salivary gland tumor in Japan." Tissue Antigens. 50. 66-70 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Asako Ando: "Triplet repeat polymorphism within the NOTCH4 gene located near the junction of the HLA class II and class III regions in narcolepsy." Tissue Antigens. 50. 646-649 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] mizuki Ohno: "Tripex-forming DNAs in the human interphase nucleus visualized in situ by polypurine/polypyrimidine DNA probes and anti-triplex antibodies. Triplex-forming DNAs are spatially and sequence-specifically associated with distinct centromeres." (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yosihisa watanabe: "Borders of early and late replication zones are present in and around the humanX-inactivation center (XIC) : correlation with chromosome band zones." (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 大野 みずき: "Non-B DNA structures spatially and sequence-specifically associated with individual centromeres in the human interphase nucleus." Chromosomes Today. 13(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 池村 淑道: "Genome and subnuclear organization of non-B forming DNAs in mammalian chromosomes." Cytogenet Cell Genet. 81. 101-102 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 椎名 崇: "Nucleotide sequencing analysis of the 146 kb segment around the IKBL and MICA genes at the centromeric end of the HLA class I region." Genomics. 47. 372-382 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 生田 智樹: "Structural analysis of mouse tenascin-X:evolutionary aspects of reduplication of FNIII repeats in the tenascin gene family." Gene. 217. 1-13 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 大野 みずき: "Triplex-forming DNAs in the human interphase nucleus visualized in situ by polypurine.polypyrimidine DNA probes and anti-triplex antibodies.Triplex-forming DNAs are spatially and sequence-specifically associated with distinct centromeres." (submitted).

    • Related Report
      1998 Annual Research Report
  • [Publications] 渡辺 良久: "Borders of early and late replication zones are present in and around the human X-inactivation center(XIC):correlation with chromosome band zones." (submitted).

    • Related Report
      1998 Annual Research Report
  • [Publications] 安藤 麻子: "cDNA cloning,Northern hybridization and mapping analysis of a putative GDS(guanine nucleotide dissociation stimulator of G proteins)-related protein gene at the centromeric ends of the human and mouse MHC regions." Immunogenetics. in press (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 天前 豊明: Mol.Cell.Biol.17. 4043-4050 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 菅谷 公彦: Gene. 189. 235-244 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 安藤 麻子: Tissue Antigens. 50. 646-649 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 椎名 隆: Genomics. in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 安藤 麻子: Tissue Antigens. 50. 66-70 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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