Mechanism of biosynthesis of a major glycolipid in thylakoid membrane and it's role in formation of chloroplast

• Project/Area Number: 09640765
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: OHTA Hiroyuki Tokyo Institute of Technology, 大学院・生命理工学研究科, Associate Professor (20233140)
• Co-Investigator(Kenkyū-buntansha): AWAI Koichiro Tokyo Institute of Technology, Doctor Course student
• Project Period (FY): 1997 – 1998
• Project Status: Completed (Fiscal Year 1998)
• Budget Amount: ¥3,000,000 (Direct Cost: ¥3,000,000); Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: Chloroplast / Glycoliped / Glycosyltransferase / Thylakoid / Monogalactosyldiacylglycerol

Research Abstract

1) Two different types of MGDG synthase genes (type A and B) were isolated from Arabidopsis thaliana.
2) Tissue specific expression profiles of type A and B MGDG synthase gene were determined.
3) Vectors for transformation of Arabidopsis and tobacco were constructed.
4) A cDNA of MGDG synthase was isolated from spinach.
5) Enzymatic properties and Localization site of spinach MGDG synthase was determined.
6) Biochemical characteristics of recombinant MGDG synthase were determined, and they are basically same to those of native enzyme purified from spinach leaves.
7) We found that spinach MGDG synthase was located in inner envelope fraction.

Research Products

• [Journal Article] Biochemical and topological properties of type A MGDG synthase, a spinach chloroplast envelope enzyme catalyzing the synthesis of both prokaryotic and eukaryotic MGDG (1999)
  • Author(s): Miege, C. Marechal, E., Shimojima, M., Awai, K., Block, M. A., Ohta, H. Takamiya, K., Douce, R., Joyard, J
  • Journal Title: Eur. J. Biochem. 265 Pages: 1-13
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Biochemical and topological properties of type A MGDG synthase, a spinach chloroplast envelope enzyme catalyzing the synthesis of both prokaryotic and eukaryotic MGDG (1999)
  • Author(s): C. Marechal, E., Shimojima, M., Awai, K., Block, M.A., Ohta, H. Takamiya, K.,Douce, R., Joyard, J
  • Journal Title: Eur. J. Biochem 265 Pages: 1-13
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Plant MGDG synthases : their evolutionary aspects and light and hormona lcontrol of the mRNA expression during developmental stage (1998)
  • Author(s): Ohta, H., Shimojima, M., Awai,K., Tatsumi, T., K. anai, D., Masuda
  • Journal Title: Advances in Plant Lipid Research Pages: 367-370
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular identification of the MGDG synthase from spinach chloroplast envelope (1998)
  • Author(s): Miege., C., Marechal, E., Block, M. A., Shimojima, M., Awai, K., Ohta, H., Takamiya, K., Joyard, J
  • Journal Title: Advances in Plant Lipid Research Pages: 247-250
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cloning of the gene for the monogalactosyldiacylglycerol synthase and it's evolutionary origin. (1997)
  • Author(s): Shimojima, M., Ohta, H., Iwamatsu, A., Masuda, T., Shioi, Y, Takamiya, K.
  • Journal Title: Proc. Natl. Acad. Sci. USA 94 Pages: 333-337
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 蛋白質・核酸・酵素 (1997)
  • Author(s): 太田啓之, 下嶋美恵, 粟井光一郎, 高宮建一郎
  • Journal Title: 葉緑体チラコイド膜糖脂質の分子構築-そのメカニズムと起源- Pages: 2601-2612
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 高等植物の主要糖脂質の合成を担う糖転移酵素-その遺伝子クローニングがもたらすもの- (1997)
  • Author(s): 太田啓之, 下嶋美恵, 増田 建, 高宮建一郎
  • Journal Title: ブレインテクノニュース 60 Pages: 5-10
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cloning of the gene for the monogalactosyldiacylglycerol synthase and it's evolutionary origin (1997)
  • Author(s): Shimojima, M., Ohta, H., Iwamatsu, A., Masuda, T., Shioi, Y.,Takamiya, K.
  • Journal Title: Proc. Natl. Acad. Sci. USA 94 Pages: 333-337
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Monogalactosyldiacylglycerol synthase : cloning of the gene from cucumber and immunological characterization of the enzyme from spinach. (1997)
  • Author(s): Shimojima, M., Ohta, H., Kitagawa, E, Iwamatsu, A., Masuda, T., Takamiya, K.
  • Organizer: Fifth International Congress of Plant MolecularBiology.
  • Place of Presentation: Singapore
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] cDNA cloning of Arabiodopsis Monogalactosyldiacylglycerol synthase, the key enzyme for glycolipid synthesis in chloroplast membrane. (1997)
  • Author(s): Awai, K., Ohta, H., Shimojima, M., Masuda, T., Takamiya, K.
  • Organizer: Fifth International Congress of Plant Molecular Biology.
  • Place of Presentation: Singapore
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] MGDG合成酵素一遺伝子の発現および植物間における酵素の比較- (1997)
  • Author(s): 太田啓之, 下嶋美恵, 粟井光一郎, 北川史二郎, 増田建, 高宮建一郎
  • Organizer: 第10回植物脂質シンポジウム
  • Place of Presentation: 九州大学
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 太田啓之: "高等植物の主要糖脂質の合成を担う糖転移酵素-その遺伝子クローニングがもたらすもの" ブレインテクノニュース. 60. 5-10 (1997)
• [Publications] 太田啓之: "葉緑体チラコイド膜糖脂質の分子構築-そのメカニズムと起源-" 蛋白質・核酸・酵素. 42・16. 2601-2612 (1997)