| Project/Area Number |
09640765
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
OHTA Hiroyuki Tokyo Institute of Technology, 大学院・生命理工学研究科, Associate Professor (20233140)
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| Co-Investigator(Kenkyū-buntansha) |
AWAI Koichiro Tokyo Institute of Technology, Doctor Course student
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Chloroplast / Glycoliped / Glycosyltransferase / Thylakoid / Monogalactosyldiacylglycerol |
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| Research Abstract |
1) Two different types of MGDG synthase genes (type A and B) were isolated from Arabidopsis thaliana.
2) Tissue specific expression profiles of type A and B MGDG synthase gene were determined.
3) Vectors for transformation of Arabidopsis and tobacco were constructed.
4) A cDNA of MGDG synthase was isolated from spinach.
5) Enzymatic properties and Localization site of spinach MGDG synthase was determined.
6) Biochemical characteristics of recombinant MGDG synthase were determined, and they are basically same to those of native enzyme purified from spinach leaves.
7) We found that spinach MGDG synthase was located in inner envelope fraction.
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