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cDNA cloning of abscisic aldehyde oxidase in Arabidopsis and Nicotiana

Research Project

Project/Area Number 09640775
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionTOKYO METROPOLITAN UNIVERSITY

Principal Investigator

KOSHIBA Tomokazu  TOKYO METROPOLITAN UNIVERSITY, 理学研究科・助手(現・助教授) (80117704)

Co-Investigator(Kenkyū-buntansha) SEKIMOTO Hiroyuki  TOKYO METROPOLITAN UNIVERSITY, 大学院・総合文化研究科, 助手 (20281652)
KOMIYA Yuji  Rekon, ホルモン機能研究チーム, チームリーダー (30100051)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Keywordsabscisic acid / aldehyde oxidase / Arabidopsis thaliana / Nicotiana plumbaginifolia / cDNA cloning / abscisic aldehyde / trans genic plant / cDNA クローニング
Research Abstract

Aldehyde oxidase (AO) has been extensively investigated in animals and microorganisms. In plants, however, there was only a limited information on this enzyme. A great interest in this enzyme has been in its possible involvement in abscisic acid (ABA) biosynthesis. In the present study, we cloned four full-length AO cDNAs from Arabidopsis thaliana and investigate their physiological functions, and we purified and characterized a leaf AO of Nicotiana plumbaginifolia. The summary of the results are as follows ;
(1) Four different putative AO cDNA clones were obtained from A.thaliana. Their products were analyzed by using antibodies each specific to four AOs in A.thaliana.
(2) The four AO cDNAs were introduced into yeast Pichia pastoris and the products were functionally expressed in the yeast cells. The result revealed that two AO genes encoded three AOs in A.thaliana seedlings and another AO gene produced a leaf AO.Biochemical studies using AOs expressed in yeast indicated that leaf AO is a most possible candidate of an AO that catalyzes oxidation of abscisic aldehyde to form ABA.
(3) However, partially purified N.plumbaginifolia leaf AO showed a very weak preference to abscisic aldehyde. This result with combination of the results from A.thaliana suggests that there might be present another AO in N.plumbaginifolia leaves, which involves in ABA biosynthesis.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Sekimoto, H,et al.: "Cloning and molecular characterization of plant・・・・" J.Biol.Chem.272. 15280-15285 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Seo, M.et al.: "Higher activity of an aldehyde oxidase in・・・・" Plant Physiol.116. 687-693 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sekimoto, H.et al.: "Molecular cloning and characterization of・・・・" Plant Cell Physiol.39. 433-442 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akaba, S.et al.: "Aldehyde oxidase in wild type and aba1 mutant・・・・" Plant Cell Physiol.39. 1281-1286 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Omarov, R.T.et al.: "Aldehyde oxidase in roots, leaves and seeds of・・・・" J.Exp.Bot.50. 63-69 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Momiyama, M.et al.: "Effects of α-irradiation of eloagation and indde-3-・・・・" Environ.Exp.Bot.(印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 小柴共一: "植物ホルモンのシグナル伝達(福田等監修)" 秀潤社, 13/212 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sekimoto, H., Seo, M., Dohmae, M., Takio, K, Kamiya, Y.and Koshiba, T.: "Cloning and molecular characterization of plant aldehyde oxidase" J.Biol.Chem.272. 15280-15285 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akaba, S., Leydecker, M.-T., Moureaux, T., Oritani, T.and Koshiba, T.: "Aldehyde oxidase in wild type and aba1 mutant leaves of Nicotiana pulumbaginifolia" Plant Cell Physiol.39. 1281-1286 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sekimoto, H., Seo, M., Kawakami, N., Komano, T., Desloire, S., Marion-Poll, A., Chaboche, M., Kamiya, Y.and Koshiba, T: "Molecular cloning and characterization of two aldehyde oxidase in Arabidopsis thaliana" Plant Cell Physiol.39. 433-442 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Seo, M., Akaba, S., Oritani, T., Delarue, M., Bellini, C., Caboche, M.and Koshiba, T.: "Higher expression of an aldehyde oxidase in the auxin overproducing superroot mutant of Arabidopsis thaliana" Plant Physiol.116. 687-693 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Omarov, R.T., Akaba, S., Koshiba, T.and Lips, S.H.: "Aldehyde oxidase in roots, leaves and seeds of barley (Hordeum vulgare L.)" J.Exp.Bot.50. 63-69 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Momiyama, M., Koshiba, T., Furukawa, K., Kamiya, Y.and Sato, M.: "Effects of gamma-irradiation on elongation and indole-3-acetic acid level of maize (Zea mays) coleoptiles" Environ.Exp.Bot.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Seo, M.et al.: "Higher activity of an aldehyde oxidase in..." Plant Physiol.116. 687-693 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sekimoto, H.et al: "Molecular cloning and characterizatin of..." Plant Cell Physiol.39. 433-442 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mitsuhashi, N.et al.: "Effect of γ-radiation on..." Phytochem.48. 1281-1286 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kondoh, K.et al.: "γ-Irradiation damage to the foroplast in..." Environ.Exp.Bot.39. 97-104 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wada, H.et al.: "Involvement of peroxidase in differential..." Plant Sci.139. 109-119 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Akaba, S.et al.: "Aldehyde oxidase in wild type and..." Plant Cell Physiol.39. 1281-1286 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小柴共一(共著): "植物ホルモンのシグナル伝達" 秀潤社, 26-38 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sekimoto,H.et al.: "Cloning and molecular characterization of plant・・・・." J.Biol.Chem.272. 15280-15285 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Seo,M.et al.: "Higher activity of an aldehyde oxidase in・・・・." Plant Physiol.116(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sekimoto,H.et al: "Molecular cloning and characterization of・・・・." Plant Cell Physiol.39(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mitsuhashi,N.et al.: "Effect of γ-radiation on the plasma・・・・." Phytochem.47(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kondoh,K.et al.: "γ-Irradiation damage to the touoplast in・・・・." Environ. Exp.Bot.(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wada,H.et al.: "Involvement of peroxidase in differential・・・・." Plant Sci.(印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2018-02-02  

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